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538 Uppsatser om Gene expression - Sida 16 av 36

Försök till att lösa degraderingsproblem vid preparation av fotosystem I-subenheten PSI-N genom att använda proteasinhibitorer och olika sorters lysis

Fotosyntesen kallas den process som omvandlar ljusenergi till kemisk energi. Fotosyntesen sker i tylakoidmembranet och drivs av två stora proteinkomplex, fotosystem II (PSII) och fotosystem I (PSI) då de tillförs energi i form av fotoner. PSI-N är ett mindre protein på ca 10 kDa som ingår i PSI. På något sätt, som ännu inte är klarlagt, samverkar PSI-N med PSI-F och plastocyanin när det dockar till PSI. Det är därför av viktigt att rena fram större mängder av PSI-N för att få djupare kunskaper om proteinet samt dess struktur och funktioner.

?Det här är inte hat ? det här är humor!" : En visuell textanalys av makt och performativitet i systrarna Kronlöfs video SÅ JÄVLA PK!

This essay examines how humor is used in the making of stereotypes in the Kronlöf sister?s video SÅ JÄVLA PK! In addition I give my analysis of how power and resistance is shown in the video. Through Michel Foucault?s conception power and resistance together with Judith Butler?s theories about performativity I?d like to show how the characters you can see in the video, in addition to the humor, also put themselves in a public context. I discuss my material in relation to a number of similar and current events that concern sexism and racism in Sweden.

Klimatcertifiering av livsmedelsföretag : -  En kvalitativ studie om vad som får företag att anta klimatmärkningen och vad det innebär

This essay is a study of the motives and driving forces affecting how a food producer looks at, and incorporates, an environmental-labeling and its impact on their organization and strategy. Driving forces and motivations is an important part of a business strategy and impacts its content and its expression. Sustainability can be part of the overall company strategy but could also define the entire company. This study has shown that the main motive for working with eco-labelling is a genuine interest in issues concerning sustainability. This creates an opportunity for the company to communicate their environmental policies to their customers. For a company that works proactive and innovative with sustainability and climate issues, the new climate framework does not mean any major organizational or strategic changes.When the framework is incorporated in the organization the farms need to be analyzed with a climate impact perspective. This essay shows that this process, together with tools developed to handle the framework, make change in the way dairy farmers think and how they relate to their daily work..

Infectious bronchitis in Brazil : a minor field study in Bastos municipality, Sao Paulo

Infectious Bronchitis (IB) is a highly contagious viral respiratory and productiondepressant disease distributed world wide. The disease is caused by the InfectiousBronchitis Virus (IBV), a positive RNA strand Avian Coronavirus within thegenus Gammacoronavirus. Due to a combination of high mutation rate and atendency of recombination a constant emergence of new genotypes and serotypesis seen. In Brazil, a country with an extensive poultry production and majorproblems with disease control, the diversity of circulating strains are wide andoutbreaks of clinical disease despite intensive use of vaccines are common. Theaim of this study was to investigate the presence of IBV and to identify genotypesof isolated IBVs in a limited number of layer flocks in Bastos, São Paulo, as partof the screening for circulating genotypes in the country.

Begomovirus susceptibility in wild Nicaraguan tomato populations

Tomato production is a common and important agricultural activity in Nicaragua. During the last decades tomato producers have suffered great yield losses due to begomoviruses (family Geminiviridae). The genome of begomoviruses generally consists of two circular ssDNA components, DNA-A and DNA-B. Tomato yellow leaf curl virus (TYLCV) is an example of a begomovirus with only one component. An approach to manage the virus epidemics has been to breed a cultivar with begomovirus resistance genes, which have been transferred from wild tomato.

Graffiti : kontextualitet, platsbundenhet och innehåll

The focus of this essay is to investigate if there lies a difference between graffiti art that is placed in the public spaces and graffiti art that is sited in an institutional art setting; in this essay exemplified in a gallery space. My thesis is that graffiti art derives a great deal of its meaning and substance from its situation consequently making a change of cultural context also a change of connotation and understanding of the graffiti art itself.  Meaning that graffiti art situated in a communal space is different from graffiti art situated in the gallery space. They share aesthetic expression but their connotations differ; i.e. one being illegal and the other permissible making their meanings dissimilar. I have used a comparison between site-specific art and graffiti art to further strengthen my thesis concerning the importance of understanding how a change of context critically changes the substance of graffiti art..

Genome editing with the CRISPR Cas9 system

Rapporten ingår i ett FoU- projekt för Högskolan Dalarna, där målet är att ta fram en konstruktion utan ångspärr som ska klara dagens mått på lufttäthet och fuktkrav.Syftet med denna rapport är att utreda hur fukt påverkar en byggnad medmassivträstomme och olika isoleringsmaterial utan ångspärr. Mineralull och träfiberisolering jämförs mot varandra för att se hur dessa påverkarfuktbelastningen i en väggkonstruktion. Testobjektet är lokaliserat i Dalarna, inget fukttillskott har funnits inomhus i byggnaden.För att genomföra detta arbete har tre stycken olika metoder används. Ensimulering, verkliga uppmätta värden och en provtagning. Fuktsimuleringen genomfördes med hjälp av programmet WUFI, uppmätta värden i form av relativ fuktighet och temperatur har samlats in kontinuerligt under två års tid från väggkonstruktionen via mätsensorer.

Mediernas konstruktion av en politisk händelse En kritisk diskursanalys av två svenska morgontidningars medierapportering av oroligheterna till följd av presidentvalet i Kenya

News value is an essential part of which event media chooses to report about. Political events consider to bee news with potentional large value. Media has an enormous power and possibility to create news and construct there own reality and where the readers is given a false expression of what really happened. Today the media must show profit and that indirect affects how the media choose to describe political events. In the Kenya case is it therefore possible to draw the conclusion that the two news paper has different construction of the political event in Kenya.

Detektion av mRNA från en cytokrom c gen belägen i genklustret för kloratmetabolism i Ideonella dechloratans

Abstract:In previous experiments with the bacterium Ideonella dechloratans a probable sequencecoding for a cytochrome c protein (cyt c) was observed, the sequence is assumed to have afunction in the bacterial respiratory chain. Detection attempts have been made to try to findthis protein, but have not succeeded. The purpose of this project is to detect the expressionof mRNA associated with the cytochrome c. And if it is detected, it will also be investigated ifthere is any difference in expression of the mRNA due to aerobic or anaerobic environment.Total RNA was purified from cultures grown under aerobic and anaerobic conditions. cDNAwas then synthesized using reverse transcriptase and subsequently amplified with thespecific primers in a qRT-PCR.qRT-PCR testing showed a distinct amplification of primer product, confirming that I.dechloratans expresses mRNA coding for the cyt c protein.

Vad är nyttan med en ökad specialisering?

Syftet med detta examensarbete är att undersöka vilken nytta den nya gymnasieutredningens förslag om ökad specialisering och förbättrad anställningsbarhet kan ha för elever inom Hantverksprogrammet ?Textil & Mode . Gällande frågan om bedömningen av en sådan nytta, har fokus i undersökningen i första hand inriktats på vilka krav och önskemål om kompetens som finns inom hantverks- och konfektionsbranschen idag men även i ett framtidsperspektiv. Undersökningen bygger på en kvalitativ forskningsansats med utgångspunkt från ett antal halvstrukturerade intervjuer med olika verksamheter inom hantverk och konfektion. De gene-rella resultaten visar på att kraven för anställningsbarhet inom den mer kvalificerade kärn-verksamheten, ser ut att ligga bortom det grundläggande kunskapsuppdrag som yrkesutbild-ningen inom gymnasiet kan avses ha.

Gröna tak som del i ett hållbart stadsbyggande

Green roofs have seen a revival in the society of today. They provide many functions of which both society as a whole and the individual can take part. In particular extensive green roofs, that requires little or even zero maintenance, provides very valuable functions. Green roofs affect our environment and our economy in a positive manner as well as the physical expression of buildings. In this bachelor thesis the impact of green roofs on society has been examined throughout literature studies. The result as a compilation of contemporary research in this field is presented together with a discussion about the use of green roofs in general. The two major arguments for an extended use of green roofs are, the high stormwater retention capacity and the cost-benefits seen over time compared to a conventional roof.

På drift med Spinoza och Freud

This essay attempts to examine whether it is possible to find a mutual understanding of the concept of drive between Spinoza?s philosophy and Freud?s psychoanalytical theory. Former texts on this subject have given a variety of conclusions: from a radical separation between the two authors to a complete identification between the two. The drive, or the desire which is the term Spinoza uses, has in Spinoza?s philosophy its foundation in the concept of conatus.

Hur påverkar kvalitetskontroller revisorers arbetsrutiner?

The purpose of this study is to describe and analyze how auditors are affected by quality controls and if audit practice changes because of quality controls. To fulfill the purpose of this study there has been interviews made with five auditors. The conclusion by this study is that the interviewed auditors work practices are affected by quality controls and that the extent and effect depends on the auditor and the office that the auditor works in. The auditors who worked as quality reviewers at the agencies where they are employed at, we felt that they were more secured in the response towards quality controls. This may be because they are quality reviewers themselves and they know what the quality control requires and what must be met to be approved.

Lust och disciplin: Medelålders kvinnors konsumtion i det senmoderna samhället

The purpose was to look into the pattern of consumption among middleage women. Our questions were how women experienced the need to consume, how they defined the expression of consumption and if there was a difference between "shopping" and "buying". The research has been done through eight qualitative interviews and searching in litterature, illuminating our questions.All women defined consumption as satisfying basic needs. According to them everything bought on a daily bases is consumption. More difficult to define was the experience of "need".

Stop motion som uttryckssätt : En kvalitativ studie med fokus på de påverkande faktorerna för varför unga vuxna amatörer väljer stop motion-animation som uttryckssätt

Playfulness and curiosity in the usage of new technology and digital filmmaking has always been of great importance within these fields. There are many different media technologies that can form what the user wants to communicate in a certain context. One of these forms of expression that has increased in popularity from the early 20th century until today is the animation technique, Stop motion. Amateur filmmakers have also found an interest in using the technique. Our purpose has been to study the different aspects as to why young-adult amateur filmmakers have chosen stop motion as the way to express themselves.

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