The problem of finding targeted medicine is a central problem in chemotherapy. From this point of view the ubiquitin-proteasome system is a highly promising object in the pharmaceutical approach. Proteasome plays a critical role in cellular protein degradation, cell cycle and apoptosis regulation.Proteasome inhibitors are substances blocking the actions of proteasome. Cancer cells are more sensitive to inhibition of the ubiquitin-proteasome system than normal cells. Therefore proteasome inhibitors have the potential to be successfully used in the cancer treatment.The study aimed to test various substances to identify possible proteasome inhibitors with the IncuCyteTM FLR image system and fluorometric microculture cytotoxicity assay.

The purpose of this study was to develop a reliable molecular-genetic method to determine the different species of helicobacter in dogs. The study is part of a larger project to map the prevalence of Helicobacter spp in healthy and sick dogs in Sweden, and to determine the possible connection of Helicobacter spp infection with gastrointestinal diseases in dogs. Several published studies have reported on the prevalence of Helicobacter spp in dogs. The problem is that three of the most common species are so alike that a 16SrRNA-PCR with sequencing is not able to differentiate between them. In this study, DNA has been purified from samples and then a Multiplex PCR has been performed. Multiplex PCRs use multiple primers in one single PCR.

De neutrofila granulocyterna ingår i det icke specifika immunförsvaret och fagocyterarmikroorganismer och rester från kroppens egna celler. Neutrofilen innehåller fyra olika typerav granula, som vid aktivering av cellen kan uppregleras till plasmamembranet. Proteinetdysferlin har bevisats förekomma i membranet i en del av dessa granuler. Det tros ha en roll imembran fusion och underhåll av membranet.Dysferlin är ett transmembrant protein av typ II, och proteinet saknas i funktionellt tillståndvid sjukdomstillstånden Limb-Girdle muskeldystrofi 2B och Miyoshi myopati. I arbetetkommer en metod att utvecklas som skall användas till att studera proteinet dysferlin hos deneutrofila granulocyterna vid olika funktionella tillstånd.

The worldwide industrial demand of energy, often in form of fossil fuel, is continuously growing and at the same time these resources are coming to an end (Hamamre, 2013). The interest and focus in renewable sources are then more important than ever and plants are suggested to be a good alternative for production of raw material for industrial purposes (Nilsson et al., 1998). But the world is also facing an increasing world population which also increase the nutritional needs, plants therefore need to be suitable for both industrial and nutritional purposes. To achieve this, common plants need to be improved but it also need new potential crops to be developed and introduced (Carlsson et al., 2009). Modern technology like gene engineering is an important tool to achieve these challenges (Carlsson et al., 2011). This work focused on analyzes and evaluation of different transgenic lines of field pepperweed (Lepidium campestre L.).

The main goal with this thesis report was to come up with ideas of how to make the use of energy more effective and thus save money for the company.First a survey was made to find out where and how the energy consumers were divided within the company. The results were then used in calculations to find out how much money that could be saved and how long it would take to get back the investments.There is a big potential in saving money by recovering heat from the UV-lamps with a heat exchanger. It would also even out the pressure in the factory which is a source to heat loss and a cause for discomfort amongst the employees. It?s possible to save 105 000 kr in a year and the money needed for the investments is 600 000 kr.By using the recovery air more effectively the company could save up to 250 000 kr a year in lowered heat purchases, but this would require the use of automatic dampers.If you reschedule the running times for the wood chipper and invest in some kind of load management, for example Sydkraft?s ?Energidirigent? you can save at least 42 000 kr.

The present study concerns a concept development of a product idea that Bengt Magnuson, associate professor, had when a heavy storm caused a widespread power failure of long duration. With a basic power-producing unit one should be able to get through a power loss. The product could also be useful for other tasks if different modules are connected, e g grass mower or snow-blower.According to the product idea the basic unit will be able to produce electric power enough for a fridge, freezer, TV, and a few electric lamps. Normally the basic unit can be used e g as a mower, when such a module is attached. An increased number of tasks would make the contraption useful and tempting for those who do not own an electrical power set in reserve.

This study presents a survey of the seroprevalence of Toxoplasma gondii and Neospora spp. in equids in three municipalities in Pará, a Brazilian state with the Amazonian rainforest and the Amazon River as dominating features. Toxoplasma gondii and Neospora spp. are two closely related parasitic protozoans not separated as different genera until 1988. Neospora spp.

Zambia is a country in Southern Africa where poverty is widespread and the traditional keeping of local poultry therefore plays a crucial role in improving nutrition. Meat and eggs from poultry are important sources of protein but also of income. Improving the poultry production is thereby a method to fight poverty. For poultry, the access to light is an important factor for both growth and egg production. In this paper, the focus is on the traditional village chickens used for both egg and meat production, how they are kept, their health and welfare and if the use of a solar lamp will influence their behaviour, growth and egg production. Three studies were conducted in Chibombo and Choma Districts in Zambia were 17 farmers were interviewed regarding the village chicken management of which six farmers participated in a solar lamp experiment.

?Halmstads Fastighets AB? is a local real estate company investing in sustainable development. One problem though is that the domestic waste, particularly the source separation, at the three resident blocks for students? isn´t working very well. This creates inconveniences for people and environment as well as decreasing the impression on the estates.This project has had the ambition to improve the domestic waste and increase the amount of source separation at HFAB´s student hostels.

The housing sector uses 40 % of the energy in Sweden and half of the electricity usage. Theuse of energy is the main reason to the increasing rate of carbon dioxide in the atmosphere.This is the main motive but also a wish of higher safety, that is a higher rate of selfsufficientenergy production in Sweden, we want to decrease the need of using fossil fuel. Now whenthe energy price rise, the interest in energy issues increase and more people discuss thepossibility of reducing the usage of fossil fuel. A change to renewable energy such as sun,wind and water is the only solution where we can get a sustainable development.One way to contribute to a better use of energy is to build in the principle of passive house.The house is being built with a thick and tight insulation. If you use a heat-exchanger withhigh degree of efficiency and a final heating battery on the ventilation system, the heat fromlamps, devices and the ones living in the house should be enough to fulfil the criteria ofliving.

Today, equine breeding is based on performance and conformation, resulting in a vast variation in fertility among different horses, with noticeable economic losses as a consequence. The horse breeding industry is therefore in need of methods that diagnose the spermatozoa concerning their quality and provide the best sperm for AI. The commonly used selection methods for sperm today are: centrifugation, where extender is added, the sample is centrifuged and then resuspended; swim-up self-migration; adherence separation; and density gradient centrifugation. However, none of these methods are in used routinely in practice before inseminating mares with fresh or cooled semen. The aim of this study was to evaluate a new selection method, Single Layer Centrifugation (SLC), and its effect on sperm viability. The viability was analyzed with regard to membrane integrity and membrane stability of ejaculated spermatozoa.

The aim with this thesis is to make a comparison between forcing tulips in a hydroponic system and the forcing off tulips that have been planted in soil. The results are mostly based on interviews with six Dutch and one Swedish tulip forcer and the managing director at the Horticultural Research Centre, Proeftuin Zwaagdijke, in the Netherlands. There are benefits and disadvantages with both methods. Tulip bulbs planted in soil seem to get a brighter colour after forcing, compared with those forced ?in water?.

Companies constantly strive for the perfect order which enables them to satisfy and retain their customers. This is a difficult process that can be facilitated by using different picking technologies. In this study, a research of the picking technologies Pick-to-Light and Pick-to-Voice have been made to investigate when each technology is suited.In order to collect relevant information and data to be able to answer the question formulation, a qualitative study with interviews have been made. The purpose of this essay was to investigate Pick-to-Light and Pick-to-Voice to see when each technology is best suited and to see what the differences between the technologies are. With our essay we wanted to help manufacturing companies to make the right decisions when implementing a picking technology.The results showed that those picking technologies give companies a higher picking quality and an increased work efficiency since the order picking is faster to perform, and that picking errors are reduced.

I dagens samhälle blir det allt mer intressant att spara energi, dels med tanke på miljön och dels föratt minska på utgifterna. Denna energi ska sparas på ett sådant sätt att effekten för användarna blirså liten som möjligt.I denna rapport beskrivs hur ett reglersystem kan byggas för att minska energiförbrukningen hosväxthus genom att reglera belysningen på ett effektivt sätt. Systemet är helt automatiskt vilket göratt användarna inte behöver tänka på styrningen av lamporna alls när väl systemet är uppsatt ochkonfigurerat.För att styra lamporna på ett bra sätt mäts det inkommande ljuset med en PAR-sensor. Lampornatänds och släcks därefter beroende på ljusmängden och även på hur varmt, eller kallt, det är iväxthusen.Styrningen av belysningen sker med en mikrokontroller från Microchip, en PIC18F4620 som ärprogrammerad i C18. Kringkomponenter som portexpasioner, klocka och reläer användes också föratt bygga ett bra reglersystem.Resultatet är att det är möjligt att bygga ett reglersystem med begränsad budget som styr lampornapå ett effektivt sätt.

I dagens samhälle blir det allt mer intressant att spara energi, dels med tanke på miljön och dels föratt minska på utgifterna. Denna energi ska sparas på ett sådant sätt att effekten för användarna blirså liten som möjligt.I denna rapport beskrivs hur ett reglersystem kan byggas för att minska energiförbrukningen hosväxthus genom att reglera belysningen på ett effektivt sätt. Systemet är helt automatiskt vilket göratt användarna inte behöver tänka på styrningen av lamporna alls när väl systemet är uppsatt ochkonfigurerat.För att styra lamporna på ett bra sätt mäts det inkommande ljuset med en PAR-sensor. Lampornatänds och släcks därefter beroende på ljusmängden och även på hur varmt, eller kallt, det är iväxthusen.Styrningen av belysningen sker med en mikrokontroller från Microchip, en PIC18F4620 som ärprogrammerad i C18. Kringkomponenter som portexpasioner, klocka och reläer användes också föratt bygga ett bra reglersystem.Resultatet är att det är möjligt att bygga ett reglersystem med begränsad budget som styr lampornapå ett effektivt sätt.