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124 Uppsatser om Fluorescence in situ sequencing - Sida 5 av 9
Molecular testing of raspberry plants infected with tomato black ring virus
Nematode-transmitted plant viruses (such as nepoviruses) cause great economically losses in commercially important plants such as tomatoes, grapevines and raspberries all over the world. To avoid spread of the viruses the interest in mapping the viruses has increased. One of the important nepoviruses infecting European red raspberries (Rubus idaeus) is Tomato black ring virus (TBRV). TBRV has earlier been confirmed in raspberry samples from Belarus by ELISA and the aim of this bachelor degree project was to confirm these results by amplifying, cloning and sequencing the coat protein (CP) gene of the virus. In total eight viral cDNA samples, from two different raspberry samples, were amplified and sequenced.
Förekomsten av den genetiska varianten laktapersistens hos neolitiska grupper från Öland : The contribution of the genetic variant Lactase persistence among Neolithic people from the Baltic island Öland in Sweden
This study deals with the contribution of the genetic variant lactase persistence among Neolithic people from the Baltic Island Öland. Skeletal remains from twelve individuals went through DNA sequencing in order to find the mutation that allows adult individuals to digest milk sugar. The twelve individuals were chosen from two different Neolithic sites, where the archaeological and isotopic data suggest that the individuals from Köpingsvik were hunters and gatherers and the individuals from Resmo were early farmers. The individuals with the genetic variant lactase persistence can be described with selection and genetic flow. Only five individuals produced results and the mutation was found in two of the subjects. All the individuals who were successfully sequenced came from Resmo, whereasno individuals from Köpingsvik yielded any results.
Restspänningar i arc-förångade (Ti(1-x)Al(x))N ytskikt
This report is the written documentation of a project work carried out at the Division of Engineering Materials at Luleå University of Technology in cooperation with Seco Tools AB. The project deals with the analysis of residual stresses in Ti-Al-N thin film grown on WC/Co cutting inserts. The composition of Al varied from 0% up to 75%. Increasing the Al content in the Ti(1-x)Alx N compound improves the compressive residual stresses even after annealing of the material. The experiments have been performed in a high temperature x-ray diffractometer (HTXRD) where the interplanar atomic spacing was determined in-situ and subsequent converted into stresses.
Occurence of mould and mycotoxins in Swedish maize silage - a pilot study
During the last ten years the cultivation of maize in Sweden has increased and is expected to grow further. Most of the maize in Sweden becomes silage which is used to feed animals at farms. Maize has in other countries been shown to be a substrate for growth of mould and especially Aspergillus spp., Fusarium spp. and Pencillium spp. has been reported.
Siktning som saneringsmetod för metallförorenad mark
Toxic metals contaminate soil worldwide and thus serve as sever environmental threat. Therefore the purposes of this study were to investigate in which soil fractions that different heavy metals (Fe, As, Cu, Zn and Pb) could be found in contaminated soils and if it is possible to use sieving as a method for decontamination. Soil samples were collected from three different locations, the Nasa silver mine, the Blaiken-mine and Svalget environmental station. The samples were oven dried and later on sieved into six different fractions 8mm, 4 mm, 0.5 mm, 0.250mm, 0.063 mm and <0.063 mm. The fractions 4 mm, 0.5 mm and <0.063 mm from each location were analyzed in an x-ray fluorescence detector.
Immobilisering av metaller i grundvatten: En utvärdering av befintliga metoder
Grundvatten är idag den viktigaste naturliga källan till dricksvatten. En fördel med grundvatten är att det är mer skyddat mot föroreningar och yttre påverkan i jämförelse med ytvatten. En nackdel är dock att om det blir förorenat är det väldigt svårt att återställa grundkvaliten. I dag är förorenat grundvatten ett stort problem över hela värden. Föroreningarna härstammar ofta från antropogena verksamheter, såsom deponier, gruvor, spill eller felaktig kemikaliehantering.
Analysis of Raspberry ringspot virus in raspberry by amplification of RNA-2
So far, only limited research has been carried out on viruses infecting raspberry, even though the diseases that these viruses cause result in economical losses for raspberry growers. Raspberry ringspot virus (RpRSV) belongs to the family Comoviridae and the genus Nepovirus. Nepoviruses are characterized by their icosahedral virus particles and bipartite genome of single-stranded (+) RNA (RNA-1 and RNA-2). These viruses are transmitted mainly by nematodes of the genus Longidorus, which feed on the roots of plants. RpRSV causes disease in raspberry, grapevine, strawberry and many other plants.
Svårigheter vid färgundersökning av arkitekturbundet måleri Fallstudie: Färgundersökning av en lågerhuggen gördellist i södra valvet på Stockholms slott
This bachelor thesis discusses various difficulties with architectural paint research. The difficulties are exemplified through a case study ? a paint research on a furrowed string-course in the southern vault of the Royal Palace of Stockholm, which was carried out November ? March 2009-2010 on behalf of Statens Fastighetsverk. The paint research was made in order to receive information about the string-course and to get inspiration for a renewed color scheme on it. The base material of the string-course is Gotlandic sandstone and the surface of it is furrowed.
An image-based method for identification of new inhibitors of Signal Transducer Activator of Transcription 1
Background: Chemotherapy and radiation resistance are major causes of failure in cancer treatment. The response to treatment in cancer cells depends on several mechanisms and pathways such as Janus kinases-signal transducers and activators of transcription JAK/STAT pathway. STAT1 was the first described transcription factor in the STAT family. STAT1 is activated by stimulation of signaling proteins such as type II interferon (IFN- ?) and the activated STAT1 translocates from cytoplasm to nucleus.
Cementstabilisering av Biltemas andra lagerbyggnad i Halmstad
This is a technical report about cement stabilization in general and the stabilizationprocess during the construction of a warehouse in Halmstad, Sweden in particular and iswritten at the section for economy and technology at Halmstad University incollaboration with NCC Anläggning.NCC encountered variations in compression strength in the stabilized material on the firstwarehouse that they constructed for the company Biltema. In our report we used thestabilization process of the second warehouse for the same company as a reference todetermine what could be done to get a more homogeneous result for future projects.We also looked in to what testing method is the most suitable to determine the result ofthe stabilization.After interviews with people involved in the process and visits on the work site we havecome to the conclusion that the most probable cause to the variations in compressionstrength is segregation in the ballast material. This segregation can occur in severaldifferent occasions in the process such as during the production, transportation andhandling on the work site.Other causes for the variation can be improper compaction, overlapping stabilization insome places and different duration of hardening of the samples that are tested.The best way to do in situ test of the stabilized material is to do seismic investigations asthose are non-destructive tests in comparison to taking core samples and you can discoverany cracks or air bubbles in the material..
Fotodynamisk terapi vid behandling av skivepitelskarcinom hos katt :
Photodynamic therapy is a relatively new treatment for superficial squamous cell
carcinoma in cats. There are no publications indicating that the method is being
used in veterinary practice in Sweden at the present time. The method uses the
interaction between a photosensitive agent which has been administered to the
tumor area, light of a wavelength that can be absorbed by the photosensitiser and
the presence of oxygen. In this study photodynamic treatments were performed at
the Queen?s Veterinary School Hopital of Cambridge University between 1998
and 2002.
Prevalens och genetisk karaktärisering av afrikansk svinpestvirus i vektorer och tamsvin i Uganda
African swine fever, ASF, was first described 1921, in Kenya. The virus has thereafter spread through many African countries as well as in Europe, Asia, South- and Central America. The virus is spread via three different cycles, of
which one is sylvatic and includes warthogs and soft ticks. The other two are between domestic pigs and the one most common one is caused by humans, who not always realize the seriousness of precautions. The infection manifests as
hemorrhagic fever, but can also be more or less unnoticed.
The aim of this study was to use molecular tools to detect and partly genetically characterize ASFV both in soft ticks and in serum from domestic pigs in Uganda.
Comparing viruses detected in ticks with those detected in domestic pigs I also wanted to investigate possible links between the sylvatic and domestic cycle.
Biologisk behandling av oljeförorenad jord : Abiotiska faktorers inverkan och effekter av olika uppläggningssätt på nedbrytningshastigheten.
The purpose of this report was to investigate how the abiotic factors (temperature, pH, nutrients, oxygen levels and water content) would affect the decomposition of oil-contaminated soil and how a variance in structured soil would affect the decomposition-rate. A minor field-study was made and soil where structured in five different ways (one was a default pile and was used as a reference). The study took place over 137 days and three samples were taken (start, middle, end) and analyzed for aromatic and aliphatic compounds. The result of this study shows that there is a significant difference between all methods for the aliphatic compounds and no significant difference for the aromatic compounds. The abiotic factors that affect decomposition vary from organism to organism, depending on what type of environment it will function the best.
Textila Konstruktioner i Rymddimensioner
Mitt arbete handlar om mötet mellan rysk konstruktivism och science fiction. Detta har jag gestaltat i ett textilt rum inspirerad av science fictionkarraktären Doktorn ur Doctor Who. Doktorn är en utomjording av en långt mer utvecklad art och från en mer avancerad civilisation än människorna, som reser i tid och rum i sitt tids-och rymdskepp TARDIS. Jag har designat ett rum som är min textila tolkning av TARDIS innersta. Arbetet kretsar kring tankar om gestaltning av en flerdimensionalitet som bara kan anas, inte ses eller förstås.
Geokemin inom osorterade cirklar : Analys av tungmetallers distribution inom jordprofiler tagna från Abiskoområdet, nordvästra Sverige
The Arctic region appears as a pristine remote environment, yet there is increasing evidence that it is greatly impacted by pollution such as lead (Pb) and mercury (Hg). In this environment deposition of Hg has increased threefold since the last industrial revolution. In this study five cryosolic soil profiles within non-sorted circles (NSC) in the Abisko region, northwest of Sweden, has been analyzed to improve our understanding regarding how pollutants are distributed within such type of soil. The soil samples have been analyzed with the use of X-ray fluorescence analysis (XRF), for the measurements of the total concentrations of e.g. lead (Pb) and zirconium (Zr).