Prolifererande verrukös leukoplaki, PVL, är en ovanlig sjukdom som yttrar sig genom multipla, vita förändringar i munslemhinnan som recidiverar och med tiden riskerar att utvecklas till cancer. Diagnosen kan endast ställas kliniskt när sjukdomsförloppet pågått under lång tid och det finns heller inga effektiva behandlingsmetoder. Etiologin till sjukdomen är okänd, men idag vet man att det finns ett samband mellan cancerutveckling och vissa virustyper. Syftet med studien är att undersöka om det går att påvisa humant papillom virus, HPV, i vävnadsprover från patienter som kan misstänkas ha PVL. Urvalet bestod av 11 patientfall, där vävnadsprover från biobanken vid avdelningen för oral patologi, Malmö högskola, samlats in och studerats med kromogen in situ hybridisering. Resultaten visade en högre förekomst av HPV i biopsier tagna i det senare skedet av sjukdomen jämfört med tidigare biopsier.

Promoters are genetic elements that facilitate the transcription of a gene and they have been found in front of non-coding RNA (ncRNA) genes in different organ-isms, e.g. the model plant Arabidopsis thaliana. A similar element, DUSE, has been found in front of ncRNAs in the social amoeba Dictyostelium discoideum and a part of this project has been to analyze the function of this putative promoter element through cloning and expression studies. A construct to analyze the func-tion of DUSE was successfully designed and introduced into D. discoideum but full expression studies were not finished because of shortage of time.

Promoters are genetic elements that facilitate the transcription of a gene and they have been found in front of non-coding RNA (ncRNA) genes in different organ-isms, e.g. the model plant Arabidopsis thaliana. A similar element, DUSE, has been found in front of ncRNAs in the social amoeba Dictyostelium discoideum and a part of this project has been to analyze the function of this putative promoter element through cloning and expression studies. A construct to analyze the func-tion of DUSE was successfully designed and introduced into D. discoideum but full expression studies were not finished because of shortage of time.

Promoters are genetic elements that facilitate the transcription of a gene and they have been found in front of non-coding RNA (ncRNA) genes in different organ-isms, e.g. the model plant Arabidopsis thaliana. A similar element, DUSE, has been found in front of ncRNAs in the social amoeba Dictyostelium discoideum and a part of this project has been to analyze the function of this putative promoter element through cloning and expression studies. A construct to analyze the func-tion of DUSE was successfully designed and introduced into D. discoideum but full expression studies were not finished because of shortage of time.

Bakgrund: Att erhålla en perifer venkateter (PVK) är idag en rutinmässig åtgärd inom hälso- och sjukvården. Risken för infektion anses som liten, men trots det inträffar allvarliga komplikationer , varav en är tromboflebit som kan leda till sepsis. Syfte: Syftet var att identifiera riskfaktorer för tromboflebit hos vuxna patienter med perifer venkateter. Metod: Metoden var en systematisk litteraturstudie. Sökning av artiklar gjordes i databaserna Cinahl och PubMed. Totalt användes 14 artiklar till studien. I analysen framkom sex olika områden som behandlade riskfaktorer för tromboflebit. Resultat: Motstridiga resultat påvisades när det gällde tid in situ, placeringen av PVK, val av storlek samt om patienten var kvinna eller man.

Inledning Implantatbehandlingar har blivit allt vanligare och material som associeras med implantat är oftast dentalt titan och dentala keramer. Även kompositmaterial förknippas ibland med implantat. Det är inte helt ovanligt att kompositmaterial förekommer vid submukösa implantatbehandlingar och få studier är hittills gjorda på kompositmaterials cytotoxiska påverkan på omkringliggande vävnad. Material och metod Sammanlagt tillverkades 24 stycken provkroppar till studien av två kompositmaterial (Crea.lign och SinfonyTM) och ett fältspatsporslin (Noritake). Tre provkroppar av varje material ytanalyserades med hjälp av optisk interferometri.

The purpose of this study was to develop a reliable molecular-genetic method to determine the different species of helicobacter in dogs. The study is part of a larger project to map the prevalence of Helicobacter spp in healthy and sick dogs in Sweden, and to determine the possible connection of Helicobacter spp infection with gastrointestinal diseases in dogs. Several published studies have reported on the prevalence of Helicobacter spp in dogs. The problem is that three of the most common species are so alike that a 16SrRNA-PCR with sequencing is not able to differentiate between them. In this study, DNA has been purified from samples and then a Multiplex PCR has been performed. Multiplex PCRs use multiple primers in one single PCR.

The escalating usage of pyrethroids has resulted in an increased awareness about resistance towards pyrethroids in insects. Pyrethroids inhibit voltage-sensitive sodium channels (VSSC) in nerve cell membranes and are composed of synthetic molecules based on pyrethrins present in pyrethrum extracts from Chrysanthemum species. VSSC are transmembrane proteins that are important for electric signalling over the membrane in insects. Mutations in the gene encoding the sodium channel have proved to be a common reason for resistance against pyrethroids. Pyrethroid resistance among pollen beetles is spread all over Sweden and also abroad and is increasing.

Flera av jordens kattdjur står just nu inför hot om utrotning, och bevarandeprojekt bedrivs såväl in situ som ex situ för att förhindra detta. Pallaskatt (Felis manul) är för närvarande listad som starkt hotad enligt CITES. Bevarandeprojekt på arten bedrivs bland annat i europeiska djurparker, där man genom forskning hoppas kunna öka katternas möjligheter till överlevnad. Denna studie utfördes på två pallaskatter på Parken Zoo i Eskilstuna, och fokuserade på hur katterna utnyttjade hägnet, samt deras aktivitetsbudget. Studien hade även som ursprungligt syfte att undersöka bohålepreferens hos katterna, men då de i hägnet utplacerade bohålorna besöktes väldigt sällan av katterna, kunde studien inte visa på något resultat här.

Biogas is a renewable energy source that is produced by anaerobic digestion of organic mate-rial. In Sweden, biogas predominately comes from sewage water sludge and landfills or from organic waste of households and industries. Small scale digestion plants at farms are espe-cially expected to contribute to increased biogas production in the future. Biogas can be ob-tained directly in it?s raw form and used as fuel in a combustion chamber.

A today common soil reinforcement method is the installation of lime-cement columns. Lime-cement columns have been used since the 1970?s and have been a useful method for stabilizing soil at great depths. It is mostly used for stabilizing road and railway embankments because the method is economically viable and the columns are easy to install. The total costs for the installation of the lime-cement columns is dependent on the numbers of installed columns, what percentage of lime and cement that has been used and the pattern in which the columns has been installed.

X-ray fluorescence (XRF) detection of heavy metals is a cost- and time-effective method for investigation of polluted areas. Compared to laboratory analysis, XRF analysis is limited by high detection limits and uncertainties in some situations. Preparation of samples is known to affect the results of measurements.The purpose of this thesis is to bring a deeper understanding of how different factors affect the results of XRF-analysis. A large number of measurements have been made with the instruments Niton XLt 700 and Niton Xli 700. Results from measurements of lead, zinc and copper have been analysed.This study has shown that a greater moisture content will give a lower measured concentration for the same sample.

Sweden signed, in 1992, the Convention on Biological Diversity (CBD) and has thus assumed responsibility for the indigenous biodiversity. That also means that Sweden has a conservation responsibility for the Swedish Friesian (SLB) (SJV, 2007a). In a survey conducted by the Board of Agriculture (SJV) in 1996, it appeared that there were around 780 cows and heifers of the SLB breed (SJV, 1997). SJV did in 2006 a plan for the development of livestock genet-ic resources and the number of cows of SLB was estimated at approximately 200. This meant that the SLB was in need of being conserved and SJV assumed it would be categorized as threatened.The primary objective of conservation of a breed is to reduce the loss of the original genetic variation (Lacy et al., 1995).

Sweden signed, in 1992, the Convention on Biological Diversity (CBD) and has thus assumed responsibility for the indigenous biodiversity. That also means that Sweden has a conservation responsibility for the Swedish Friesian (SLB) (SJV, 2007a). In a survey conducted by the Board of Agriculture (SJV) in 1996, it appeared that there were around 780 cows and heifers of the SLB breed (SJV, 1997). SJV did in 2006 a plan for the development of livestock genet-ic resources and the number of cows of SLB was estimated at approximately 200. This meant that the SLB was in need of being conserved and SJV assumed it would be categorized as threatened.The primary objective of conservation of a breed is to reduce the loss of the original genetic variation (Lacy et al., 1995).

Language impairment affects the ability to communicate. Children with languageimpairment have difficulties in using language in interaction to various degrees, and the contributions of the conversational partner are of great importance. The use of languagein interaction can be analysed by means of conversation analysis. The purpose of thepresent study was to study interaction of a group of pre-school children with language impairment with four different conversational partners. These were a peer with languag eimpairment, a peer with typical language development, a pre-school teacher and a parent.