This study was conducted at the City Abattoir in Kampala, Uganda to evaluate the cleaning process, with focus on bacterial pathogens, in a pilot scale integrated bioprocess. This consisted of four steps: anaerobic sequencing batch reactors; aerobic/anoxic sequencing batch reactors; a high performance temperature controlled methanogenesis digester and a constructed wetland. The objective was to determine if this type of cleaning process could be used to clean wastewater from abattoirs in a satisfactory way. The indicator organisms for faecal contamination, Escherichia coli and Enterococcus spp. were used to investigate in what numbers bacteria could be found in the untreated wastewater and how much that was reduced in the different steps of the cleaning process. The water was analysed for Salmonella to see if human pathogens could be found and could survive the different steps of the treatment process.

The Yangtze finless porpoise (Neophocaena phocaenoides asiaorientalis), is a small freshwater cetacean which has recently fallen dramatically in number. It is an endemic species for the Yangtze River and unless drastic action is taken, it risks the same fate as the now functionally extinct Yangtze river dolphin (Lipotes vexillifer). Threats to the porpoise are diverse but they have in common that they are caused by man. Development of the river has been made to supply a growing human population with food, hydropower, and to reduce the risk of flooding. These changes have resulted in a degraded habitat for the porpoise.

Leber´s hereditary optic neuropathy (LHON), a disease affecting vision, is caused by several point mutations in mitochondrial DNA. Mutations leading to a defect NADH ubiquinone oxidoreductase protein will affect the respiratory chain and cause a disturbed ATP production. It is still unknown why this defect leads to the degeneration of retinal ganglion cells and cells in the opticus nerve as well as demyelination of axons in these areas. Analysis of mitochondrial DNA is an important tool in the diagnosis of the disease. At the present time analysis is based on cleavage by restriction enzymes, which only detects two of the most frequent mutations: m.3460G>A and m.11778G>A.

Multiple Congenital Ocular Anomalities (MCOA) is a genetic disease that affects primarily Silver coloured horses of breeds such as the Rocky and Kentucky mountain horses. In these breeds the Silver dapple colour is very popular leading to an increase of affected horses. The major feature of the disease is ocular cysts of variable size. Large cysts also lead to a variety of secondary syndromes, for example retinal detachment. In previous studies the locus for MCOA has been mapped to an interval of 420 kb on equine chromosome six.

The aim of the project was to investigate if Swedish rodents, brown rat (Rattus norvegicus), house mouse (Mus musculus), wood mouse (Apodemus spp) and water vole (Arvicola terrestris) captured in pig herds and in urban areas, constitute a reservoir for pathogenic Leptospira spp. Under Swedish conditions leptospirosis gives rise to reproductive disorders in pigs and symptoms of flu in humans. It has long been established that rodents are chronic carriers of pathogenic leptospires in their kidneys, and can spread the bacteria with urine. The old nomenclature of serovoars is still in use, but a new system of genetic classification has developed and today the genus Leptospira contains 17 species. There are several diagnostic test developed for leptospirosis.

The aim of the project was to investigate if Swedish rodents, brown rat (Rattus norvegicus), house mouse (Mus musculus), wood mouse (Apodemus spp) and water vole (Arvicola terrestris) captured in pig herds and in urban areas, constitute a reservoir for pathogenic Leptospira spp. Under Swedish conditions leptospirosis gives rise to reproductive disorders in pigs and symptoms of flu in humans. It has long been established that rodents are chronic carriers of pathogenic leptospires in their kidneys, and can spread the bacteria with urine. The old nomenclature of serovoars is still in use, but a new system of genetic classification has developed and today the genus Leptospira contains 17 species. There are several diagnostic test developed for leptospirosis.

The aim of the project was to investigate if Swedish rodents, brown rat (Rattus norvegicus), house mouse (Mus musculus), wood mouse (Apodemus spp) and water vole (Arvicola terrestris) captured in pig herds and in urban areas, constitute a reservoir for pathogenic Leptospira spp. Under Swedish conditions leptospirosis gives rise to reproductive disorders in pigs and symptoms of flu in humans. It has long been established that rodents are chronic carriers of pathogenic leptospires in their kidneys, and can spread the bacteria with urine. The old nomenclature of serovoars is still in use, but a new system of genetic classification has developed and today the genus Leptospira contains 17 species. There are several diagnostic test developed for leptospirosis.

With a growing population on earth the amount of end-of-life vehicles (ELVs) and other scrap steel increases. The European Directive 2000/53/CE? requires a minimum of 95 % reuse and recovery and 85 % re-use and recycling no later than 1 January 2015, for end-of life vehicles. This imposes a need for more efficient recycling and recovery. Despite this shredder residue (SR) is commonly land filled.This report investigated the magnetic fines residue generated at Stena Metall shredder facility in Halmstad, Sweden.

This thesis has examined the Tekla Structures modeling software and answered the question of whether it could act as a suitable modeling program for the design department at Bjerking AB in Uppsala. The method used in this work is a case study of a previous project with existing basis to work form. The demarcation of the study was to examine only the usability of the foundations of in situ concrete. The work examined three main areas within the design process. The first was to model the concrete elements then to model the reinforcing and ultimately production of the drawings. Because Autodesk Revit was the existing modeling program in the Department conducted the evaluation as a comparison between the two programs.

In situ sekvensering är en metod som kan användas för att lokalisera differentiellt uttryck av mRNA direkt i vävnadssnitt, vilket kan ge viktiga ledtrådar om många sjukdomstillstånd. Idag förloras många av sekvenserna från in situ sekvensering på grund av det kvalitetsmått man använder för att säkerställa att sekvenser är korrekta. Det finns troligtvis möjlighet att förbättra prestandan av den nuvarande base calling-metoden eftersom att metoden är i ett tidigt utvecklingsskede. Vi har genomfört explorativ dataanalys för att undersöka förekomst av systematiska fel och korrigerat för dessa med hjälp av statistiska metoder. Vi har framförallt undersökt tre metoder för att korrigera för systematiska fel:I) Korrektion av överblödning som sker på grund avöverlappande emissionsspektra mellan fluorescenta prober.II) En sannolikhetsbaserad tolkningav intensitetsdata som resulterar i ett nytt kvalitetsmått och en alternativ klassificerare baseradpå övervakad inlärning.III) En utredning om förekomst av cykelberoende effekter, exempelvisofullständig dehybridisering av fluorescenta prober.Vi föreslår att man gör följande saker:Implementerar och utvärderar det sannolikhetsbaserade kvalitetsmåttetUtvecklar och implementerar den föreslagna klassificerarenGenomför ytterligare experiment för att påvisa eller bestrida förekomst av ofullständigdehybridisering.

Apart from recognizing and neutralizing threats, antibodies also have the ability to regulate immune responses against their specific antigen. IgG administered together with erythrocytes is well known to induce >99% suppression. This has been used to prevent haemolytic disease in new-borns since the 1960's, but the mechanism behind it is still not completely understood. The effect on the primary response is well established, but its influence on induction of immunological memory is still heavily debated. In this study adoptive cell transfer of splenocytes to irradiated mice was used to survey the effect IgG has on induction of immunological memory without disturbance from the primary IgG response.

Syftet med detta projekt var att studera inverkan av gröda och skördetidpunkt på kemisk sammansättning och vomnedbrytning av neutral detergent fibre (NDF) och torrsubstans (TS) i helsäd samt att jämföra ANKOM DaisyII in vitro-metoden med in situ-metoden med avseende på nedbrytningsförloppet i vommen av NDF och TS i helsäd. Havre, korn, rågvete och vårvete odlades i storrutor med tre upprepningar i fält. Hela försöket upprepades under två år (2002 och 2003). Varje storruta delades upp i två smårutor, som skördades vid tidig mjölkmognad respektive tidig degmognad. In vitro-inkubering skedde med ANKOM DaisyII-inkubator av 48 prover från båda åren.

With a growing population on earth the amount of end-of-life vehicles (ELVs) and other scrap steel increases. The European Directive 2000/53/CE? requires a minimum of 95 % reuse and recovery and 85 % re-use and recycling no later than 1 January 2015, for end-of life vehicles. This imposes a need for more efficient recycling and recovery. Despite this shredder residue (SR) is commonly land filled.This report investigated the magnetic fines residue generated at Stena Metall shredder facility in Halmstad, Sweden.

The effect of inoculation on containerized pine and spruce seedlings in a nursery with two ectomycorrhizal fungi was investigated in regard to the development of mycorrhiza and plant growth. The fungi were Elaphomyces muricatus and Elaphomyces granulates and the inoculated seedlings were compared to an uninoculated control. These seedlings were all fertilized with arginine. Five mycorrhiza species were found after DNA sequencing of the mycorrhizal root tips with different found morphotypes on seedlings from all treatments. Thelephora terrestris was the type which had the greatest distribution on pine seedlings and Tylospora asterophora was found at big extent on spruce seedlings. The inoculated species was not found, if it was due to the species hadn?t colonized the roots or if the DNA sequencing failed we can only speculate about.

Uppsats för avläggande av filosofie kandidatexamen i Kulturvård, Bygghantverk, 22,5 hp 2014.