The ability to detect protein-based biomarkers, which are linked to different diseases like colorectal cancer, is very important as a diagnostic tool. Usually complex biological samples like blood are studied which will contribute to different technical issues when performing an assay. The aim with the project is to optimize and develop the high throughput protein detection technique Proximity Extension Assay, PEA, into a 96-plex panel, in hopes of discovering an expression profile for colorectal cancer. PEA was developed by Olink Bioscience and allows specific proteins in a sample to be quantitatively transformed into nucleic acid sequences that are subsequently detected and quantified with real-time PCR. Two proximity probes containing oligonucleotide sequences bind pairwise to target protein and when brought in proximity, a DNA polymerase will extend a hybridization arm from one probe over to the second forming a double-stranded DNA sequence that can serve as a template in real-time PCR.

The problems around and the planning of wood flow is some of the most complex issues in the forest-sector. The experience and overview of the single employees is crucial for the possibility of correct tactical and economical decision-making. The increasing demand of savings and shorter reaction-times with greater flexibility in the wood-supply-chain, makes solutions and aids for improved effectiveness in the work and processes according to wood-flow urgent. Holmen Skog is starting to develop a new forest-road-management-system. The system will facilitate the work according to construction, improvements and updating the standard of roads.

Most of the portable electronic devices use a battery as a primary power source. Nowadays, when the applications are becoming smaller and smaller and more power efficient, the designer needs to concentrate more on reducing power consumption, using secondary power sources and introducing power storage devices. Multiple power source availability and a power storage device on the board will make the applications much more useful and efficient in everyday use. This thesis proposes a power supply unit (PSU) for wireless sensor platforms which is capable of supplying a sensor node with energy from multiple sources, as well as status information from the PSU. Present power storage devices, like electric double-layer capacitors (EDLCs) and batteries, have become more and more common as their characteristics have developed.

SAMMANFATTNINGBakgrund: Läkemedelsbiverkningar utgör ett stort problem för den enskilde som drabbas och för samhället. Distriktssköterskor med förskrivningsrätt har ett ansvar att rapportera misstänkta läkemedelsbiverkningar och spontanrapportering är grunden för läkemedelssäkerhet. Studier visar att det finns en hög grad av underrapportering. Syfte: Syftet med studien var att undersöka distriktssköterskors kunskap och förståelse om läkemedelsbiverkningar samt deras handlingsberedskap för, och syn på att rapportera dessa. Metod: Semistrukturerade intervjuer med kvalitativ ansats användes, sju distriktssköterskor i Jämtlands län deltog.

Background: Hepatitis E (HEV) is a small, non-enveloped virus that belongs to the viral genus Hepevirus. HEV is a positive sense single-stranded RNA virus and there is insufficient information regarding its replication. This is mainly because the virus has low capacity to grow in normally used cell cultures. Many specific strand assay detection studies have been done in order to understand more about HEV replication. Unfortunately, these assays have the disadvantage of giving false positive results.Aim: The aim of this project was to improve the positive strand assay to increase specificity and eliminate false positivity which is due to high sensitivity of the polymerase chain reaction (PCR).

The aim of the study was to examine individual staff experience and management of threats and violence in the acute psychiatric department for children and teenagers in Uppsala. Previous research shows that the experience of threats and/or violence is individual, and that it is difficult to distinguish between these concepts. Research shows that it is particularly vulnerable to work in psychiatry when mental illness is a cause when threats and violence occurs. Threats and violence can result in different consequences for the individual. The study is based on qualitative interviews and the material was collected in the autumn of 2012 at Akademiska sjukhuset.

AbstractIntroductionWedged shoe insoles are used as a treatment for different problems in order to change the ground reaction force. They can secondary cause different loading patterns under the foot compared to normal. No previous study has, to the author?s knowledge, clinically evaluated the plantar foot pressure with different full-length wedges in normal persons.PurposeThe purpose was to evaluate the plantar pressure with different degrees of full-length wedges in normal persons.MethodTwenty persons with normal feet have participated in this single blind study. Wedges were produced clinically in four different shapes, 3° valgus, 0° flat, 3° varus och 6° varus.

The overarching purpose of the study was to examine the effects of infant massage and to put these effects incontext with mother ? child attachment. The intention was also to study whether these effects have any relevancefor social work. This was accomplished by studying earlier research, theory and laws but also through interviewswith mothers who have followed through a course in infant massage. The main questions of the study was: Afterthe completion of the course and after using the massage technique at home, does the mother experience that thebond with their child is influenced by the technique and are the mothers more apprehensive of their child?ssignals? Has the mothers observed any signals from their infants during the massage and if so what has beentheir reaction? Does the mothers experience that the massage has provided any physiological effects? Has thecompletion of the course provided the mothers with knowledge that?s had an effect in their ways of parenting? Inwhat way does the earlier research, reports and laws deal with the benefits and risks of a secure or insecureattachment, and can the quality of the attachment have any meaning in the social work? A qualitative approachwas chosen with respondent-interviews.

In the early 1860´s, the author and opinion former Fredrika Bremer made a large donation of books to Högre lärarinneseminariet, which is the first governmental institute for higher education of women in Sweden. The aim of this thesis is to describe the collection of donated books as well as to extract information regarding Bremer's international network of contacts, her views on women's education and her relation to Högre lärarinneseminariet. Furthermore, the aim is to highlight the unique relationship between Fredrika Bremer's private collection of the books and the library of Högre lärarinneseminariet from a gender perspective. A handwritten list of the 321 titles constitutin the donation has been preserved in the seminar archive and three quarters of the books have been re-discovered in a bookcase at Årsta castle. The donation is characterized by its double origin as stamps and personal dedications remain as permanent traces in the books.

Insomnia is getting more prevalent with age, both because of natural changes in the sleep pattern and because of increased morbidity. It can cause dysfunctioning and sedation during the day. Therefore it is often treated with hypnotics. It is possible though that hypnotics can cause daytime sleepiness as well, not to mention increased risk of falling and fractures because of sedation and prolonged reaction time. Benzodiazepines and benzodiazepine related drugs, the subjects of this study, are commonly used as hypnotics.

This essay examines whether, and if so to which extent, the project Export MatureDestination in the Biosphere Reserve Vänerskärgården with Kinnekulle can combine the goalof the tourism industry, i.e. to double tourism between 2010 and 2020, and the goals of theBiosphere reserve, i.e. sustainable societal development and a sustainable biosphere economy.The aim is to examine how key individuals in the project approach sustainable developmentand economic growth, representing the two main interests in the area. Examining the state ofthe two interests the following examples are used: the position of sustainable development inregard to economic growth, the opportunities and challenges in the area and unity concerningthe goals of the tourism development in the region. The study is conducted through qualitativeinterviews with key individuals and a descriptive document analysis of the most importantpolicy documents.

The aim of this study is to investigate the components of religion and spirituality in the specific context of "Fryshuset" ? a multicultural and glocal arena. Against the backdrop of contemporary researchers such as Heelas and Woodhead and their hypothesis about the "spiritual revolution" and a "new spirituality", as well as the postmodern discussion about "the return of God", this study discusses how religious change can be understood in the context of Fryshuset.The theoretical point of departure is taken in a hermeneutical method where Fleck´s theory about "thought-collective" and Gidden´s theory about the "duality of structure" are used in order to understand and describe the dialectical relationship between individual and social structure.The method used in this study is qualitative. The interviews are made of six individuals representing different social projects at Fryshuset.By analyzing the material from the field study through theories on religious change, three areas are identified:"The subjective turn"Religion and spiritualityA Cosmopolitan interpretation of life The result of this study is that the religiosity among the interviewed can be described through Taylor?s understanding of the "subjective turn".

In this study we examine five selected media organisations with different forms of productionplatforms that all appear in Stockholm's southern and western suburbs, which are part of themillion-construction project. Their stated ambition is to present an alternative image of thesuburbs than that found in mainstream media. They want to portray themselves instead ofconstantly being described by others.The purpose of the study is to gain a better understanding of the reason for the emergenceof these media, their visions according to the media producers themselves, and how theyrelate to mainstream media. The study is based on a theoretical framework that focuses ondiverse approaches of alternative media and the journalists' role in a democratic society.The wide data analysis from qualitative informant interviews, with small observations as acomplement, showed results indicating criticism against the misrepresentation of Stockholmsuburbs in mainstream media. Furthermore the results show that the overall dissatisfaction ofthis misrepresentation is one of the main reasons why the different media, which are bothrepresented by citizens and journalists, were founded.

This essay discusses the students? thoughts about the teacher's response to their gender identity. The students discuss their right to have an intergender identity which means that they don?t identify themselves in the traditional sexes, man and woman, and the gender role each category has. They want to be called with a neutral pronoun and not be seen like boys or girls.

Vanlig tarmbakterie som Escherichia coli (E. coli) kan bland annat orsaka infektioner i bukhålan och urinvägsinfektioner. Infektioner orsakade av bakterien har ofta behandlats med betalaktamantibiotika, som penicilliner och cefalosporiner, vilket har resulterat i selektion av antibiotikaresistenta bakterier. Extended-Spectrum Beta-Lactamases (ESBL) är enzymer som hydrolyserar 3:e generationens cefalosporiner som cefotaxim, ceftazidim och ceftriaxon, men kan även bryta ner penicilliner, monobaktamer och övriga cefalosporiner. ESBL förekommer främst hos E.