A method for intra vitam detection of bornaviral infection and diagnosis of bornaviral dis-ease in the cat is currently lacking. The aim of this work was to explore the possibility of using the proximity ligation assay (PLA), with its capacity of recognizing femtomolar con-centrations of a protein, as a method for detection of the minute quantities of antigen and antibodies that are present in the tissues and body fluids of a bornaviraly infected cat. For this purpose reliable comparative assays, in the form of an ELISA and an IFA, had to be established. When this proved impossible within the time frame of the project, the aim of the study had to be abandoned, further underscoring the need for improved means of detec-tion of bornaviral antibodies and antigen..

Hard-to-heal ulcers are resource intensive due to the fact that they are difficult to treat and especially vulnerable to bacterial invasion. The bacterial culture contaminating these wounds often consist of several different bacterial organisms that originate from endogenous sources. Necrotic material in ischemic ulcers provide nutrition which support bacterial reproduction, increasing the risk of infection. Determining causative pathogen in infected ulcers proves to be difficult when culturing swab samples, however Staphylococcus aureus and hemolytic streptococci generally act as primary pathogens.    The aim of the study was to investigate if the detection rate increased for S. aureus and hemolytic streptococci when culturing swab samples from ulcers on Columbia CNA; a media selective for gram-positive bacteria.

In order to rescue a distressed submarine crew, the Swedish Navy is able to use a deep submergence rescue vehicle, DSRV. This mini submarine ascends, docks to the downed submarine and picks up the whole crew. The submarine rescue ship HMS Belos has a special crane located in the aft of the ship to lift the surfaced DSRV aboard, which then docks on to the on board pressure chambers located below deck. During inspections the aforementioned crane has shown to have problems with cracks in the structure, most of them located in the bolted joint between the top part of the crane and the two leg parts. A simulation of the forces acting on the structure in heavy sea condition was conducted; these values were then used as input values in a Finite Element Method, FEM, analysis of the joint.

In this diploma work the approach of determining the required amount of reinforcement in slabs exposed to pure tension is processed, on behalf of Ramböll Uppsala. It is a well-known problem in the industry that there are uncertainties in the determining of the amount of crack reinforcement, especially for structures exposed to pure tension. Cracking caused during the time of dehydration in concrete slabs is the most common defect according to statistics made by CBI[1], which provides numbers as high as 35 % of all the defects caused on slabs are generated by cracks. Comparisons between Sweden's former national standard BBK 04, Building Regulations for concrete structures due to restrained forces of shrinkage, and the current European standard Eurocode 2 are carried out through theoretical studies and concludes with several examples of calculations. Rigorous calculations of both standards, BBK 04 and Eurocode 2 with application of the national annex in Sweden and Denmark are performed.

In this diploma work the approach of determining the required amount of reinforcement in slabs exposed to pure tension is processed, on behalf of Ramböll Uppsala. It is a well-known problem in the industry that there are uncertainties in the determining of the amount of crack reinforcement, especially for structures exposed to pure tension. Cracking caused during the time of dehydration in concrete slabs is the most common defect according to statistics made by CBI[1], which provides numbers as high as 35 % of all the defects caused on slabs are generated by cracks. Comparisons between Sweden's former national standard BBK 04, Building Regulations for concrete structures due to restrained forces of shrinkage, and the current European standard Eurocode 2 are carried out through theoretical studies and concludes with several examples of calculations. Rigorous calculations of both standards, BBK 04 and Eurocode 2 with application of the national annex in Sweden and Denmark are performed.

AbstractConcrete foundation slabs are usually exposed to unilateral drying during the curing process. The unilateral drying causes an axial shrinkage and a concave curvature of the construction. The curvature results from the fact that the upper part of the slab is drying and shrinking faster than the lower part. This is called gradient shrinkage.A problem occurs when the slab is considered fully restrained by adjoining structural parts. The deformations caused by axial contraction due to the constant part of the shrinkage and the curvature caused by gradient shrinkage of the slab are then prevented by the fact that the slab is fully restrained.

Influenza is a viral infection that affects global health and economy with its endemic and sometimes pandemic spread. Rapid detection of Influenza viruses enables antiviral use and can bring financial savings. It is also essential for the global surveillance of prevalent Influenza strains. RT-PCR is considered the most specific and sensitive method for detection of Influenza, but Influenza mutates at a high rate and it is therefore crucial that RT-PCR methods are updated regularly.In 2014, Cepheid released their Xpert Flu/RSV XC assay, which can detect Influenza A and B and RSV by multiplex RT-PCR in approximately one hour. The aim of this study was to evaluate this assay at Laboratoriemedicin Västernorrland by using the laboratory?s previous PCR assay for detection of Influenza viruses as reference method.Real-time RT-PCR was used to compare Xpert Flu/RSV XC to the reference method.

IPv6 har, till skillnad från tidigare versioner av protokollet, inbyggt stöd för redundans hos gatewayenheter. Vid nyttjande av ett flertal gatewayenheter kan det, med hjälp av Neighbor Unreachability Detection, ske automatisk övergång till en ny gatewayenhet utifall att den aktiva skulle gå ur funktion. Innan IPv6 har man varit tvungen att förlita sig på externa lösningar för att uppnå denna redundans. Den huvudsakliga fråga som ställs i rapporten är om det inbyggda stödet för gatewayredundans i IPv6 är tillräckligt snabbt för att kunna nyttjas självständigt, utan att förlita sig på externa lösningar. För att kunna ta fram ett värde om vad som var ?tillräckligt snabbt? har vi, genom att läsa tidigare forskning om användares upplevelse av fördröjningar, kommit fram till att en fördröjning ej får överstiga tio sekunder.

The first part of the thesis consists of a brief introduction to the general principles of target detection and the sensor-systems used. In the following part there is a theoretical description of the algorithms this thesis focuses on. The detection algorithms described in this paper are called Cell Average, Ordered Statistics, 2parameter and Gammadetector. Two different discriminators called Extended Fractal Features and Quadratic Gamma Discriminator are also described. The algorithms are tested on three different types of data, simulated SAR-pictures, authentic SAR-targets and IR-pictures.

This report discusses a method to detect divers in harbour environments usingpassive acoustic detection. The goal was to achieve as high detection as possiblewithout having too high false alarm rate. A short execution time was also desirable.By using the characteristic of an inhalation a band-pass filter was applied to the signalto improve the signal to noise ratio. After the filtering an energy estimation of thesignal was made. This energy estimation was later used in a frequency analysis, whichcould tell us if there were enough energy in the frequencies that correspond to adivers breathing frequency.

Södra Cell Mörrum is one of the five paper pulp plants that are included in Södra Cell, and the paper pulp that is produced here is not only sold to Swedish paper mills. Most of the paper pulp is exported to different countries in Europe. In the manufacturing process the plant needs different kind of process water and there are guideline values for how much copper, iron and sodium this water is allowed to contain. Analyzes of this water is in the current situation done with an atomic absorption spectrometric instrument (AAS-instrument) with a flame. Measurements done with flame-AAS of samples that have concentrations near the guideline values for copper, iron and sodium, are not reliable. The reason for not being reliable is that the quantitation limits of these metals are higher than the limit values.

Traffic accidents where wild animals are involved represents between 60 and 80 percent of all reported accidents, depending on location in Sweden. In a country like Sweden, with a lot of forest, there is always risk of a collision with a wild animal. Imagine if you, as a road user, had the possibility to receive warnings when the risk of an accident according to statistics is extra high.Wildlife Detection Network is a wildlife warning system with an information service, which makes the whole concept unique. When an animal is approaching the road, it is registered by sensors, and warning lights along the road are lit to inform drivers of the potential danger. In conclusion, this is a direct warning to all drivers on the road where the system is placed.When an animal is registered by the sensors, information containing time, date, weather circumstances and coordinates are sent to a database.

In 1929, the Scientific Crime Detection Laboratory was established in Chicago?the first of its kind in the United States. The purpose was to engage in practical use of scientific methods in the detection of crime. In 1930, the institute published its own periodical called The American Journal of Police Science. Applying the theory of boundary-work, this essay analyses how the novelty institute argued its legitimacy as a scientific establishment through the expansion of Police Science as a collective field of knowledge.

This thesis is performed at Saab Bofors Dynamics in Karlskoga and investigates a technique for ranging with laser pulses. The investigated technique is called Constant Fraction Detection (CFD). Described briefly, the received laser pulse is split into two equal parts, where one part is delayed half the pulse width and inverted. This signal is added to the original pulse. The resulting curve has the shape of a laying S and the detection of the zero level is used to stop the time measurement.

Reliable methods to analyze food for the presence of almond are important ? not only for those allergic to almond, but also for monitoring the compliance with labelling regulations (EG directive 2003/89). Until now the Swedish National Food Administration has used methods like rocket immunoelectrophoresis and real-time PCR to detect almond in food. These methods are, however, not sensitive enough for protecting the most sensitive individuals. Therefore, the performance of a commercial ELISA kit was tested with regard to specificity/cross reactivity and limit of detection for almond both in solution and in different matrixes.The limit of quantitation was at least 3,1 ppm (mg/kg) in solution and similar concentrations were measured in bisquits and chocolate.