Diabetes mellitus is a rising epidemic throughout the world and there is currently great interest in quantifying the beta-cell mass (BCM) in vivo non-invasively. In the present experiment, the feasibility of in vivo imaging of the glucagon-like peptide-1 receptor (GLP-1R) in beta-cells was examined, using the positron emission tomography (PET) tracer [68Ga]Ga-DO3A-VS-Cys40-exendin-4 as a marker, in native pancreatic beta-cells of a porcine diabetic animal model and healthy controls. Eight Swedish high-health domestic pigs were randomly assigned to be either controls or made diabetic using streptozotocin (STZ). The experiment proceeded during eight weeks, starting with an acclimatisation period. Once the pigs had been socialised they underwent surgery for the insertion of a jugular vein catheter, allowing induction of diabetes with STZ, intravenous (i.v.) injections and stress-free blood sampling. Development of diabetes was confirmed by clinical examinations, blood glucose values and insulin-staining of pancreatic sections post mortem. The diabetic pigs were insulin treated and responded well.

Bakgrund: Typ 1 diabetes (T1D) och graviditet är en kombination förknippad med risker hos mor och barn ochdet är av största betydelse att hålla blodsockret inom normala nivåer för den gravida kvinnan. Kvantitativastudier visar att kvinnor med T1D har en hög grad av ångest, depression och negativa tankar under graviditet.För att på ett djupare plan bättre förstå upplevelser under graviditet hos kvinnor med T1D krävs kvalitativastudier. Syfte: Syftet är att genom en metasyntes analysera och integrera resultat från kvalitativ forskning kringupplevelser under graviditet hos kvinnor med typ 1 diabetes (T1D). Metod: En metasyntes baserad på dentolkande metaetnografin genomfördes. Sökningar utfördes i databaserna Cinahl och PubMed.

Bakgrund: Immobilisering a?r en vedertagen omva?rdnadsa?tga?rd inom dagens prehospitala traumava?rd, detta trots att det inte finns na?gra randomiserade kontrollerade studier anga?ende a?tga?rden. Ma?nga studier har dock visat att riskerna med immobilisering a?r ma?nga varfo?r det a?r viktigt att underso?ka omva?rdnadshandlingen na?rmare.Syfte: Syftet med studien var att underso?ka fo?rekomsten av radiologiskt faststa?llda spinala skador hos patienter som immobiliserats prehospitalt efter trauman, samt pa? vilka indikationer denna immobilisering gjordes.Metod: En kvantitativ retrospektiv studie da?r 288 journaler granskades i ambulansens journalsystem MobiMed 3.0 och i sjukhusets journalsystem Cosmic. Da?refter analyserades resultaten i SPSS deskriptivt och grafiskt, med chi2-test samt med oberoende t-test.Resultat: Resultatet visade att av de 246 patienter som genomga?tt en radiologisk underso?kning fanns en konstaterad spinal skada hos 48 patienter, vilket motsvarade cirka 17 %.

A sample of milk inoculated with S. aureus was treated with penicillin, and Antipen (a new commercial product containing penicillinase) was added. To another sample with S. aureus and penicillin, formic acid to pH 5 was added, and to yet another sample, a combination of formic acid and Antipen was added. Controls with no penicillin were treated in the same way.

Aim: The aim of this study was to compare how patients with diabetes type 2 self-estimate their self-consciousness, problem identification, stress management and the willingness to change before and after a daycare week. The aim was also to compare if the value on HbA1c have changed after the daycare week compared to before.Method: A total of 60 patients who recived offer to participate in a daycare week at a clinic on a university hospital in Sweden. Patients replied a form (SWE-DES-SF-10) before (n=60) and three months after (n=35) the daycare week. They also left a blood sample for HbA1c before (n=60) and three months after (n=42) the daycare week. The forms and HbA1c-values were analyzed using wilcoxon´s signed-rank test and paired t-test. Results: After the daycare week estimated the participating patiens their self-consciousness and problem identification higher than before the daycare week.

Milk fever, or parturient paresis, is the second most common disease in Swedish dairy cows. The disorder is associated with the onset of lactation when some cows are unable to meet the metabolic demands of calcium to support milk production and therefore develop a state of hypocalcaemia. Clinical hypocalcaemia (milk fever) may lead to coma and death in severe cases but subclinical hypocalcaemia has also been shown to have negative effects on e.g. feed intake and production and to increase the susceptibility of the cow to develop secondary diseases. The nutritional strategy applied precalving is of great importance in preventing milk fever and e.g.

The aim of the study was to examine the competence of dental hygienists among Clinic Director´s within the Public Dental Service and Private dentists in the county of Skåne . The study was quantitative and consisted of an electronic questionnaire sent out to all Clinic Directors in the county of Skåne. A total of five hundred and thirty eight questionnaires were sent out. The inclusion criteria were the Clinic directors who employed dental hygienists who had obtained their professional degree during the last four years. Twenty nine Clinic directors responded to the study.The results showed that in general clinic directors are pleased and feel that dental hygienists are proficient in periodontology and dental caries diagnostics.

Previous studies have shown positive results on the use of music as postoperative pain relief. Quantitative studies have examined the effect of music on rated pain and otherparameters such as anxiety, relaxation and blood-pressure. The purpose of this study was to investigate the expectations and experiences of patients and nurses onimplementing music as postoperative pain relief in an orthopedic unitQualitative design and semi-structured interviews were used to examine expectations and experiences. Five patients and five nurses in the unit were interviewed.Four themes developed during the data-analysis. These themes were ?Expectations on music as postoperative pain relief?, ?Experiences of music as postoperative pain relief?,?Effects of music as postoperative pain relief? and ?Continued implementation of musicas postoperative pain relief?.

A shorter dry period length has in studies abroad showed some positive effects among high yielding dairy cows. Among other things it has been suggested that the periparturient metabolic stress becomes lower, that the fertility is improved and dry off becomes gentler for the udder, due to a lower milk yield. The milk yield is generally reduced during the first period after calving, especially among primiparous cows. The aim of this master thesis was to investigate how Swedish cows were affected by a shortened dry period, regarding milk yield, metabolism, health, body condition and body weight from two months before calving to some weeks after calving. Also plasma concentration of lactose during the period before calving was determined.

The purpose of this study was to investigate the occurrence of Verotoxin-encoding bacteriophages in mussels, cultured downstream the sewage treatment plant in Lysekil.Mussels were collected in three growing areas from April 2008 to March 2009. Real-time PCR was performed for detection of vtx1 and vtx2 genes and enrichment of bacteriophages on non Verotoxin-producing Escherichia coli O157: H7 was carried out. All samples in real-time PCR analysis were negative; no presence of Verotoxin-encoding phages was shown. No plaque was formed on blood agar base plates, indicating that no bacteriophages had been taken up by E. coli bacteriaThe levels of Verotoxin-encoding phages and E.coli outside the sewage treatment plant in Lysekil were not high enough to be able to form VTEC in mussels, indicating that the faecal contamination was low.

The aim of this study was to investigate the effects of an infusion of hypertonic saline solution (HSS) in combination with an oral waterload on cows suffering from rightsided displacement of the abomasum (RDA). Six dairy cows were treated with 7,2% HSS in a dosis of 5 ml per kg bodyweight. They also received an oral waterload of approximately 15 liters. To monitor changes in fluid-, acid-base- and electrolyte-balance, bloodsamples were collected during the next 24 hours. During this period, the cows also underwent surgery to empty and correct the position of the abomasum.

Background: Heart failure is a complex condition which means that the hearts capacity topump blood is reduced. The aim of this study was to investigate how patients with heartfailure considered their knowledge and the information they received about their disease andself-care.Method: Patients suffering from heart failure, hospitalized at section 30 E (n=13), andpatients visiting the nurse led heart failure clinic (n=4) at Akademiska sjukhuset in Uppsala,were requested to participate in the study. The patients answered questions about knowledgeand information concerning self-care and their disease. Data were analyzed using SPSS 15.0.Results: The patients valued their knowledge about the disease as lower than their knowledgeabout self-care. The study showed that all the patients found the information they hadreceived about their self-care better than the information they had received about theirdisease.

SAMMANFATTNINGNatriuretiska peptider är en är en grupp proteiner som via natriuretiska, diuretiska och vasodilaterande egenskaper reglerar vätskehomeostasen i kroppen (Levin et al, 1998). De frisätts bland annat när hjärtmuskelcellerna utsätts för stress och kan fungera som biomarkör för att utvärdera hjärtsjukdom. Analysering av typ B natriuretic peptide (BNP) i blod kan komma att vara till hjälp för den praktiserande veterinären vid diagnostisering av hjärtsvikt och även vid uppskattning av grad av hjärtsjukdom. Det har även visat sig att BNP kan användas för att uppskatta prognosen hos hundar med kronisk klaffdegeneration (Chetboul et al, 2009; Moonarmart et al, 2010).Kronisk klaffdegeneration är den vanligaste hjärtsjukdomarna hos hund och drabbar fram för allt små till medelstora hundar från medelåldern och uppåt (Kogure, 1980). Atrioventrikulärklaffarna, vanligtvis mitralis, blir nodulärt förtjockade med inlagring av glukosaminoglykaner och fibrosvävnad vilket leder till klaffinsufficiens.

Feline infectious peritonitis (FIP) is a feline viral disease with high mortality. There is no cure or any effective vaccine available today. Many questions are yet to be answered about this disease and the immune response in affected cats. The aim of the study is to evaluate two different techniques for the study of cytokine profiles in cats vaccinated with a vaccine concept against FIPV. More information about the immune response in these cats could give valuable information to better understand the pathogenesis of the disease and the development of an effective vaccine.

Peritonitis is a potentially life-threatening disease in horses. With no published work from Sweden, the objective of the study was to describe the occurrence and demographics of horses with peritonitis and to evaluate the outcome of treatment in terms of short-term survival during a ten-year period in a large clinic in Sweden. Data were examined in a retrospective manner in 69 horses diagnosed with and treated for peritonitis at Universitetsdjursjukhuset (UDS) in Uppsala between 2002-2012. Demographic data examined included age, breed, gender, history, duration of illness before arriving at UDS, reason for seeking veterinary care, initial clinical findings (general state of health, heart rate, respiratory rate, mucous membrane appearance, rectal temperature, abdominal sounds, rectal examination, nasogastric tube results), abdominocentesis results (abdominal fluid analysis including visual inspection, leukocytes and protein, cytology, bacterial culture and sensitivity pattern), complete blood count (CBC), Serum amyloid-A (SAA) upon presentation and a follow up, plasma fibrinogen, plasma protein and albumin, treatments, length of hospitalisation and outcome. All medical records with the diagnosis of peritonitis were extracted from the medical records system Trofast. All records with any other diagnosis code referring to trauma such as rectal tear or ruptured uterus, recent abdominal surgery, external trauma or rupture in the gastrointestinal tract were excluded. To be included in the study, the peritoneal fluid should contain more than 20.000 cells/?L, have a peritoneal protein value of >30 g/L; or have a significantly changed peritoneal fluid sample (orange with increased turbidity or worse) in cases where no data on cells or protein were available. The horses were divided into two groups; one where the peritonitis was deemed to have an idiopathic aetiology (primary peritonitis) and the other group where the peritonitis had a possible aetiology such as intestinal parasites, impaction etc.