Human T-lymfotropt virus (HTLV) är ett C-typ onkovirus som tillhör familjen Retroviridae som huvudsakligen angriper T-lymfocyter och orsakar leukemi och andra autoimmuna sjukdomar. I den nuvarande kliniska diagnostiken används Enzyme-linked immunosorbent assay och ibland vid screeningsmetoden uppträder ospecifika reaktioner. På senare tid har flera metoder baserade på real-tids PCR utvecklats för att bestämma halten av virala genom i patienter. Syftet med denna studie var att sätta upp och optimera en realtids-PCR för detektion av humant T-lymfotropt virus typ-1 och 2. Inför optimering av realtids-PCR extraherades DNA från MT-4 och MO-T cellinjer.  Under optimering av realtids-PCR användes SYBR Green och smältpunktsanalys där flera komponenter bl.a.

Great care and knowledge are required to assess the values of public parks. Many aspects have to be considered to make the assessments reliable and credible. Care and respect for the site and its users are necessary parts of such an assessment. Stockholm City Museum releases in 2011 a document which describes a new system for assessing the cultural historical value of parks and green areas in Stockholm, a city with a great development pressure. In order to evaluate the public realm basic knowledge about the sites? history is needed. Three different parks in Stockholm have been investigated.

During the last years, the moral and ethics in companies has been a growing subject in media. Large multinational companies are to a bigger extent using workforce in developing countries and the working conditions at the workplaces in those countries, are more or less controlled. Even if a company carefully controls its own business, it can be using subcontractors that for instance use child labour or forced labour, or in some other way makes human rights abuses. When the world gets more globalized and the market where the companies act gets bigger, there will be new issues for companies to handle. The companies have to satisfy not only the interests of the costumers and the shareholders, but also the environment around it, containing many different stakeholders.

This thesis highlights starting points underlying the notions of two feminist utopians, Christine de Pizan and Charlotte Perkins Gilman. In approaching their texts, it focuses on the notions central to understand their writings. These would be historical, social, and cultural contexts. Defining concepts as utopian in its varying forms and feminism has been of significance. A feministic approach grounded in Simone de Beauvoirs philosophical phenomenology in the study of feministic utopia's visibility, has contributed to the understanding of the power structures to which women are tethered. In order to understand the way women are presented, it has become obvious that The Book of the City of Ladies and Herland are literary works that are related not only by their authors? background and personality but by the society in which they lived. Through their engagement in the contemporary intellectual debate on all social planes, both authors contributed to shifting the focus of their own contemporary notions of a woman to the notion of a woman equal to men. Woman, in Christine de Pizans utopia, is given specific properties and through themes of issues given meaning. She is not free in the sense that Simone de Beauvoir says.  She has a broader repertoire than was historically assigned, but her freedom is not arbitrary, contingent, and temporary and judged by her actions.

My purpose with this thesis has been tostudy the intersection between Landscapearchitecture and Land Art. They both relateto the landscape in some way whetherit is to the urban landscape or thenatural landscape.What tangent points are there betweenthese two subjects, and can you learnsomething of the artists approach to thelandscape?Land Art is an art movement that began inthe late 60?s, what links are there to thecurrent landscape?Last summer, I travelled around the U.S.for a month and visited the three classicLand Art projects. Lightning Field in NewMexico, by Walter De Maria, Sun Tunnels inUtah, by Nancy Holt and the Spiral Jetty,also in Utah, by Robert Smithson.The sites where visited from half a day upto 24 hours and was described and analyzedwith text and photographs.To visit these sites has been crucial formy thesis and will affect my future workas a landscape architect. The use of themethod has been a thought of mine for along time, particularly to analyze ordescribe a landscape with images.

This essay concerns the site of Pompeii and its preservation. The aim is to investigate what causes the decay of the ruins, how to prevent it and what consequences the tourism has on the site. The material consists of interviews with three Swedish scientists who are currently working with documenting a block in Pompeii. The result reveals a large number of threats against the city, such as climate, tourism and an inadequate economy situation. Examples of solutions are more investments which can be used for restorations and reconstructions, and guards to prevent vandalism.

The Swedish coastal defence took on a whole new meaning after the introduction of the coastal artillery in 1902. The establishment of the coastal artillery meant that the Swedish military had access to a whole new military branch, in addition to the Army and the Navy. Under the direction of one general, two regiments were established. One in Vaxholm and the second in the naval city of Karlskrona. The aim of this essay is to investigate how the Costal Artillery were established, by analyzing the parliamentary of the year 1901.

Solid waste management is one of the most essential functions in a country to achieve asustainable development. In India, it has been one of the least prioritized functions during thelast decades. The most common ways to treat waste in India today are open dumping anduncontrolled burning. These methods are causing severe environmental pollution and healthproblems. India is one of the world?s largest emitter of methane gas from waste disposal.Since methane is a strong greenhouse gas, even small emissions have large impact on theclimate.

Temporary architecture is an alternative urban planning concept which has been adapted in many cities worldwide during the last decade. There is however no common definition for temporary architecture and it is diversely labelled and applied. In 2013 it also became publicly introduced in Stockholm via the approval of the city's first architectural guideline. Temporary architecture is presented in the guideline as a potential tool for exploring public spaces among other architecture related themes. The ideas for temporary architecture are ambitious and promising. The problem is that they are vague and leaves questions regarding performance unanswered.

Discussions about the sustainable city are now all over the world and many believe that the solutions are merely technical. But what gives the city its character is what is happening in the public places. It is these spaces that bring the people together. Design is the key to sustainable urban spaces. We must produce public places that reflect our society.

In the year 1800, about 5 % of the world population lived in cities (World Wide Fund for Nature, 2012, 3). Today that number is about 70 % in Europe and North America, and in 2050 it is expected to be 86 % in the developed world and 67 % in the developing world (www, UN, 2009). In addition, the global population is expected to increase with 2.2 billion people until 2050. As a result of the population increase more food has to be produced which requires more transport from the countryside to the cities. However, this flow of resources faces some difficulties.

The paper evaluates two housing proposals with Space syntax as method. The proposals are very different in a topological sense but are in a configural sense almost the same. This effect is because both proposals connect themselves to the existing configural structure of the city in the same way. Neither of them takes advantages of the most integrated streets in the existing structure and use them to concentrate pedestrian and car flows to the streets or points which are intended to be the most urban areas. The knowledge of Space syntax and this evaluation puts focus on the pour understanding among architects and planners of what effect urban design proposals will have..

A manufacturing machine at the engineering company Sandvik Coromant has a positioning table which task is to move an item linearly. The positioning table has a linear motor capable of high velocity and acceleration. This makes it possible to achieve short cycle times and a more profitable production.The purpose of this thesis is to bring forward a new concept which is more adapted to the requirements of the positioning table. The reason is that the current positioning table provides an unnecessarily positioning accuracy of 0.01 mm, while the requirement for the positioning accuracy is just 0.1 mm. The goal is to reduce manufacturing and component costs.To develop a suitable solution an approach for the concept development process has been performed.

People in Sweden and in Europe have been traveling a lot more lately. Because of the economic boom that has been in a lot of countries people do have more money to spend. Sweden and especially Stockholm has lately become a very popular destination. Also big congresses and business meetings like to choose Stockholm for their meetings. The capital as it looks today does not have the capacity to let all those people stay at the hotels at the same time.

People in Sweden and in Europe have been traveling a lot more lately. Because of the economic boom that has been in a lot of countries people do have more money to spend. Sweden and especially Stockholm has lately become a very popular destination. Also big congresses and business meetings like to choose Stockholm for their meetings. The capital as it looks today does not have the capacity to let all those people stay at the hotels at the same time.