A method for intra vitam detection of bornaviral infection and diagnosis of bornaviral dis-ease in the cat is currently lacking. The aim of this work was to explore the possibility of using the proximity ligation assay (PLA), with its capacity of recognizing femtomolar con-centrations of a protein, as a method for detection of the minute quantities of antigen and antibodies that are present in the tissues and body fluids of a bornaviraly infected cat. For this purpose reliable comparative assays, in the form of an ELISA and an IFA, had to be established. When this proved impossible within the time frame of the project, the aim of the study had to be abandoned, further underscoring the need for improved means of detec-tion of bornaviral antibodies and antigen..

Hard-to-heal ulcers are resource intensive due to the fact that they are difficult to treat and especially vulnerable to bacterial invasion. The bacterial culture contaminating these wounds often consist of several different bacterial organisms that originate from endogenous sources. Necrotic material in ischemic ulcers provide nutrition which support bacterial reproduction, increasing the risk of infection. Determining causative pathogen in infected ulcers proves to be difficult when culturing swab samples, however Staphylococcus aureus and hemolytic streptococci generally act as primary pathogens.    The aim of the study was to investigate if the detection rate increased for S. aureus and hemolytic streptococci when culturing swab samples from ulcers on Columbia CNA; a media selective for gram-positive bacteria.

After the storm Gudrun in 2005, the Swedish Transport Administration started clear-cutting of railroad corridors to reduce the risk of trees falling down on the tracks. Simultaneously, train accidents with moose and roe deer have become more frequent and the costs for consequent delays and repairs of engines are of growing concern. There is reason to assume that tree-clearance of railroad corridors may have contributed to an increase in number of accidents with moose and roe deer because the cleared areas provide attractive forage and thus may attract wildlife. The objective of my study was to investigate how clearing of trees affected the number of accidents with moose and roe deer. I compared deer-train collision frequencies before and after clearance of the selected railroads and compared these with uncleared control railroads in a BACI (before-after control-impact) approach.

Influenza is a viral infection that affects global health and economy with its endemic and sometimes pandemic spread. Rapid detection of Influenza viruses enables antiviral use and can bring financial savings. It is also essential for the global surveillance of prevalent Influenza strains. RT-PCR is considered the most specific and sensitive method for detection of Influenza, but Influenza mutates at a high rate and it is therefore crucial that RT-PCR methods are updated regularly.In 2014, Cepheid released their Xpert Flu/RSV XC assay, which can detect Influenza A and B and RSV by multiplex RT-PCR in approximately one hour. The aim of this study was to evaluate this assay at Laboratoriemedicin Västernorrland by using the laboratory?s previous PCR assay for detection of Influenza viruses as reference method.Real-time RT-PCR was used to compare Xpert Flu/RSV XC to the reference method.

IPv6 har, till skillnad från tidigare versioner av protokollet, inbyggt stöd för redundans hos gatewayenheter. Vid nyttjande av ett flertal gatewayenheter kan det, med hjälp av Neighbor Unreachability Detection, ske automatisk övergång till en ny gatewayenhet utifall att den aktiva skulle gå ur funktion. Innan IPv6 har man varit tvungen att förlita sig på externa lösningar för att uppnå denna redundans. Den huvudsakliga fråga som ställs i rapporten är om det inbyggda stödet för gatewayredundans i IPv6 är tillräckligt snabbt för att kunna nyttjas självständigt, utan att förlita sig på externa lösningar. För att kunna ta fram ett värde om vad som var ?tillräckligt snabbt? har vi, genom att läsa tidigare forskning om användares upplevelse av fördröjningar, kommit fram till att en fördröjning ej får överstiga tio sekunder.

The first part of the thesis consists of a brief introduction to the general principles of target detection and the sensor-systems used. In the following part there is a theoretical description of the algorithms this thesis focuses on. The detection algorithms described in this paper are called Cell Average, Ordered Statistics, 2parameter and Gammadetector. Two different discriminators called Extended Fractal Features and Quadratic Gamma Discriminator are also described. The algorithms are tested on three different types of data, simulated SAR-pictures, authentic SAR-targets and IR-pictures.

This report discusses a method to detect divers in harbour environments usingpassive acoustic detection. The goal was to achieve as high detection as possiblewithout having too high false alarm rate. A short execution time was also desirable.By using the characteristic of an inhalation a band-pass filter was applied to the signalto improve the signal to noise ratio. After the filtering an energy estimation of thesignal was made. This energy estimation was later used in a frequency analysis, whichcould tell us if there were enough energy in the frequencies that correspond to adivers breathing frequency.

Södra Cell Mörrum is one of the five paper pulp plants that are included in Södra Cell, and the paper pulp that is produced here is not only sold to Swedish paper mills. Most of the paper pulp is exported to different countries in Europe. In the manufacturing process the plant needs different kind of process water and there are guideline values for how much copper, iron and sodium this water is allowed to contain. Analyzes of this water is in the current situation done with an atomic absorption spectrometric instrument (AAS-instrument) with a flame. Measurements done with flame-AAS of samples that have concentrations near the guideline values for copper, iron and sodium, are not reliable. The reason for not being reliable is that the quantitation limits of these metals are higher than the limit values.

In motorsports competition, safety reasons require that the vehicle is equipped with some form of protective roll cage. The roll cage should withstand the forces involved in a collision to protect the driver and the co-driver from the vehicle body deformation. The roll cage also works to protect the vehicle from excessive deformations to more easily make any repairs afterwards. For the roll cage to be certified and approved for motorsports competition, it requires that the cage is built after one of the recommended options. One of those options is to design the roll cage like a cross-construction at the driver and co-driver doors.

In 1929, the Scientific Crime Detection Laboratory was established in Chicago?the first of its kind in the United States. The purpose was to engage in practical use of scientific methods in the detection of crime. In 1930, the institute published its own periodical called The American Journal of Police Science. Applying the theory of boundary-work, this essay analyses how the novelty institute argued its legitimacy as a scientific establishment through the expansion of Police Science as a collective field of knowledge.

This thesis is performed at Saab Bofors Dynamics in Karlskoga and investigates a technique for ranging with laser pulses. The investigated technique is called Constant Fraction Detection (CFD). Described briefly, the received laser pulse is split into two equal parts, where one part is delayed half the pulse width and inverted. This signal is added to the original pulse. The resulting curve has the shape of a laying S and the detection of the zero level is used to stop the time measurement.

Under de senaste åren har en ny trend inom spelindustrin uppstått då en stor del av spelen har gåtttillbaka till de tvådimensionella miljöerna som var vanligare i tv-spelens barndom, detta tack vareatt mobiltelefoner och deras spel har tagit allt större plats på marknaden och de är oftatvådimensionella på grund av sin begränsade hårdvara. De tvådimensionella spelen har enligttradition inte haft någon dynamisk ljussättning, utan de har bestått utav färdig grafik medförutbestämda intensiteter vilket gör att spelen kan se platta ut.Men inom andra områden av spelindustrin, framförallt tredimensionella spel, har utvecklingen avdynamiska ljussättningsmetoder kommit långt. Problemet med dessa metoder är att de kräverobjektens normaler, vilka normalt inte finns tillgängligt i tvådimensionella bilder.Idag är mobiltelefonerna kraftfulla och klarar av mycket tyngre beräkningar än vad de gjorde förbara några år sedan. Därför undersökte vi möjligheten att implementera dynamisk ljussättning i etttvådimensionellt spel på androidbaserade mobiltelefoner. För att genomföra detta krävdes också attvi undersökte metoder för att generera normaler för tvådimensionell grafik.För att ett fullständigt spel skulle kunna skapas så implementerades också saker somkollisionshantering, artificiell intelligens och procedurell generering av banor..

The aim of the Master?s Thesis work is to study and develop algorithms for autonomous travel of a UGV (Unmanned Ground Vehicle). A vehicle for the mounting of sensors has been constructed in order to perform the work. Since the UGV is to be used outdoor in urban areas, GPS can be used. To improve precision and robustness, inertial navigation is used in addition to GPS, since GPS reception is likely to be diminished in such areas.

Reliable methods to analyze food for the presence of almond are important ? not only for those allergic to almond, but also for monitoring the compliance with labelling regulations (EG directive 2003/89). Until now the Swedish National Food Administration has used methods like rocket immunoelectrophoresis and real-time PCR to detect almond in food. These methods are, however, not sensitive enough for protecting the most sensitive individuals. Therefore, the performance of a commercial ELISA kit was tested with regard to specificity/cross reactivity and limit of detection for almond both in solution and in different matrixes.The limit of quantitation was at least 3,1 ppm (mg/kg) in solution and similar concentrations were measured in bisquits and chocolate.

During the last decades the fertility of dairy cows has declined in the same rate as the milk yield has increased. This is a result of the negative genetic correlation between milk yield and fertility, failure to show estrous signs and metabolic problems in the cow. The purpose of this review was to investigate the function of metabolites and hormones as markers for disturbances in fertility, health and production in the dairy cow. There are several metabolites that are suggested to indicate the cow?s energy balance.