AbstractThe research project Liberation, supported by the European Commission under the Environment and Sustainable Development Programme (Fifth RTD Framework Programme) aimed at developing a decision support system for ecological risk assessment of contaminated sites has proposed a tiered system including the triad method. This method integrates contaminant chemistry and bioavailability analyses with observed ecotoxicological effects in soil and groundwater. In this study the triad method was used to perform a risk characterization of a former surface treatment and metal industry, now used for the student union buildings of the Mälardalen University in Eskilstuna, Sweden. On the site, high concentrations of copper, zinc and nickel have been detected in the soil in a preliminary investigation of contaminant levels with XRF. For performing the risk assessment of the site according to the triad method, contaminant bioavailability, soil metal concentrations and soil toxicity were determined and an ecological assessment was carried out on four selected soil samples (one reference soil and three samples with low (B), medium (C) and high (D) contaminant levels). Contaminant bioavailability was measured through leaching (ISO TC 190/S7) and biouptake tests (DGT).

Cows affected by mastitis caused by Klebsiella (K.) pneumoniae often become seriously ill. In some cases herdproblems may occur. Contaminated bedding material is considered to be one of the most common risk factors for klebsiella mastitis. In a recently performed study in USA it was found, however, that more than 80 % of healthy, lactating dairy cows excreted K. pneumoniae in faeces.

Two species of bees in the genus Apis, real honey bees, has long been of interest for man. These two are the European honey bee, Apis mellifera, and the Asian honey bee, Apis cerana. In Vietnam, beekeeping is of great importance, both with A.cerana and A.mellifera. The aim of this project was to investigate if the introduction of the European honey bee in Asia has affected the Asian honey bee, and whether different pathogens from A.mellifera have been transferred to A.cerana. Totally 40 samples, 20 from every species, were analysed for 8 different viruses.

The moisture content in 10 deliveries of tree parts and 10 deliveries of wood residues were measured with different methods to analyze the quality of procedure used at Dåvamyran today. At the same time a survey was made on the measure procedures used at heating plants in Sweden The majority of the heating plants in Sweden use weight and moisture content as base for payment but at some plants only volume. The moisture content is usually estimated by drying wood chips in an oven but few plants a visual estimation is used. At few plants moisture content is estimated spectroscopicily. The techniques using near infra read light and other electromagnetic are still at a test stage. The methods of measurement used today are quit unreliable for the individual deliveries and there is a risk for systematic mistakes.

Platelet aggregation is a major and common problem in blood samples from cats. This greatly affects the accuracy of counting of the platelets. Because of the difficulties in counting feline platelets, results for patients and reliable reference values for cat platelets are currently so inaccurate as to be almost useless in diagnosis of thrombocytopenia. In this study we used an effective method to prevent platelet aggregation. Blood was collected in CTAD test tubes and prostaglandin E1 was added to the blood sample.

The aim of this project was to develop a product which gives one or more objects of daily use an additional function. Thru the creativity of children the object shall transform into a new source for play for pre-school children.The purpose of this work was to research how fantasy and free play of children could be enhanced. I wanted to know how I can motivate children to use their fantasy transforming objects into new sources for play.This was achieved by means of developing a number of different prototypes which I tested in Kindergarden. My observations lead to the development of a cloth-wrap for the chair which provides the required source for ongoing role-play. The chair suddenly transforms into a new landscape and multi-purpose object where children?s fantasy is stimulated and creative play is supported..

The purpose of this trial was to evaluate the possibility to use sampling of faeces, urine and plasma, and measurements of blood pressure as methods of discovering hypomagnesemia in horses, as well as the application handiness of these methods. The most commonly used method of checking the magnesium status of a horse is to analyze samples of serum or plasma. The trial was of a switch-back design, in which the horses were used as their own controls. The animal material was five Swedish warm blooded trotters between six to ten years old, and weighing between 485-620 kg. The trial consisted of three periods that were two months each.

The objective of this work was to evaluate and compare three field methods for detection of mastitis, California Mastits Test (CMT), DeLaval cell counter DCC (DCC) and FMA2001 Farm Milk Analyzer (FMA2001). Furthermore, to investigate at what cell count level clinical symptoms of mastitis are seen, and finally to check if the clinical findings and cell counts could be related to bacteriological findings. DCC and FMA2001 are new analytic instruments to be used on dairy farms for detection of mastitis. CMT has been a widely used cow-side-test of milk quality in mastitis control efforts, but it cannot be used for estimating cell counts in scientific studies or investigations. Two hundred and eight quarter milk samples from 52 cows were analysed with DCC and CMT, and 168 of these samples with FMA2001. Microbiological examination was done on 38 milk samples with CMT scores of 4 and 5.

Listeriosis, which is caused by Listeria monocytogenes, is a rare food borne disease that primarily affects people whose immune system is weakened. These may be elderly people, pregnant women, new-born infants and individuals on immunosuppressive medication. The most common symptoms are meningitis, septikemia and abortion. The mortality is high, on average 20-30 %. In recent years the number of cases in Sweden has increased.

SECO Tools manufactures tool inserts for all types of metal cutting machining. (Ti,W)C is a common raw material used in the production. At arrival, the material (Ti,W)C is always analyzed. Titanium has previously been analysed with x-ray fluorescence spectrometry, but as the calibration has previously been performed with two different types of titanium (rutile and metallic titanium), the results has differed. Because of that, this thesis project has been devoted to analyze titanium, to find the best titanium compound for producing standards.The work has been performed using literature studies and many experiments in the laboratories at SECO Tools inFagersta,Sweden.

 Genotyping can be used to link genetic variation among individuals to certain diseases or conditions. Some known disorders and states that are dependent on single nucleotide polymorphism (SNPs) are lactose intolerance, venous thrombosis, hereditary hemochromatosis and the difference in sensibility among people to metabolise drugs.In this project a new kit, TaqManÒ Sample-to-SNP KitÔ for extraction of DNA and preparation of the extract for genotyping with real-time PCR and allelic discrimination, was evaluated. QIAamp® DNA Blood Biorobot® MDx Kit was used as the reference method.The purpose of the comparison was to find a method that makes DNA extraction from blood samples cheaper and faster, but with the same reliability as the reference procedure.The results of the evaluation showed a complete agreement of the genotype results between the methods tested, which means that the new method was as reliable as the reference method. The costs of reagents and material would be reduced with 52% if the new method is adopted, that alone would result in a cost reduction of 144 000SEK a year with a sample volume of 650 samples/month. The time for DNA extraction would also be reduced with the new procedure. .

Rabies is a fatal zoonotic disease spread worldwide. The most common sources of infection for all animals and humans are bites from dogs or bats. The aim of this degree project was to diagnose and determine the source of infection for 11 rabies samples from São Paulo State and Minas Gerais State, Brazil. Diagnosis was made through direct immunofluorescence assay, mouse inoculation and RT-PCR. The sources of infection were determined by sequencing 234 nucleotides of the 5? end of the N-gene and align these with homologous sequences retrieved from GenBank.

In this final thesis, an existing method for analysis of chlorophenols (CP) in bottom sediments has been updated and adjusted for analysis of chlorophenols in soil. The covalent bonds between the chlorophenols and the soil matrix were broken through basic hydrolysis and the chlorophenols were then separated from the water phase through addition of sulphuric acid followed by ether extraction. The chromatography was improved through extractive acetylation of the chlorophenols.The updated method was then applied on soil samples from a contaminated area (a former sawmill in Hyttsjö, Östergötland, Sweden).The analyse was preformed by GC/MS with respect to 2-MonoCP, 4-MonoCP, 2,4-DiCP, 2,6-DiCP, 2,4,6-TriCP, 2,3,4,6-TetraCP and pentachlorophenol (PCP).Contamination of chlorophenols in nature can be explained by the former use of wood preservative chemicals based on chlorophenols. In the 1960s and the 1970s these chemicals were used in Sweden, but due to their toxicity they were banned by the Swedish government in 1978.In Hyttsjö a pentachlorophenol-based product named Santobrite was used for several years. The concentration of PCP in the soil samples from Hyttsjö varied from 0.2->1.8 ng/mg dry substance.

Calcareous fens constitute a specific habitat which is uncommon in Europe and protected by the EU habitat directive due to its importance to several threatened species. Hagebyhöga in Östergötland is a calcareous fen habitat included in the Natura 2000 Networking Programme. Adjacent to the existing fen, a degraded fen area is situated. The area has been used for agriculture for several years but is now going to be restored to its former state by the county administrative board of Östergötland.When working with restoration of degraded fen areas it is important to aquire knowledge about the chemical soil conditions. Plant growth limited by phosphorus and low fertility are characteristic conditions for calcareous fens, whilst arable land usually is highly fertile and limited by nitrogen.The purpose of this study is to bring forth useful knowledge for restoring the degraded calcareous fen of Hagebyhöga.

So far, only limited research has been carried out on viruses infecting raspberry, even though the diseases that these viruses cause result in economical losses for raspberry growers. Raspberry ringspot virus (RpRSV) belongs to the family Comoviridae and the genus Nepovirus. Nepoviruses are characterized by their icosahedral virus particles and bipartite genome of single-stranded (+) RNA (RNA-1 and RNA-2). These viruses are transmitted mainly by nematodes of the genus Longidorus, which feed on the roots of plants. RpRSV causes disease in raspberry, grapevine, strawberry and many other plants.