Vectors based on lentivirus backbones have revolutionized our ability to transfer genesinto many cell types. Lentiviral vectors integrate into the chromatin of target cells and do not transfer any viral genes causing vector replication. Both of these features arecommonly used in gene therapy and have been used clinically in individuals sufferingfrom cancer, infections and genetic diseases. It has been discovered that T-cells can be genetically modified to be used as effective weapons against cancer: therefore virus mustbe produced to deliver the gene of interest into the T-cells. In this project, lentiviralvectors have been produced to transfer the gene coding for a chimeric antigen receptor(CAR) which is directed to CD19 on B-cells.

Kriminalvården är den myndighet i Sverige som ansvarar för fängelser, häkten och frivård. Myndigheten har länge känt ett behov att inom flera verksamheter kunna få tillgång till delmängder av deras klientsystem i ett mobilt och uppkopplat format.Det största hindret i deras fall med mobilitet och applikationsteknik var säkerheten. Därför genomfördes en riskanalys med hänsyn till frivården och mobilapplikationsteknik.För att välja en lämplig och passande riskanalysmetod jämförde vi ett antal metoder. Det visade sig att metoden CORAS passade bäst. När vi väl genomfört riskanalysen med CORAS försökte vi sedan matcha åtgärderna mot de olika mobilplattformarnas egenskaper för att se hur de kan fullfölja åtgärderna.

Milk yield levels in dairy cows are influenced by many factors such as nutrition, breed, health status, management and environment. This study was conducted among small scale farmers in Zambia, where the climate can be dry for up to six months per year and cause a lack of green pasture for livestock and on-farm produced feed. The purpose of this study was to identify management factors that can influence milk yield at small scale dairy farms in Zambia, and present advice that could improve productivity, without the need of expensive investments. The study included semi-structured questionnaires presented to the farmers (n=29) including questions about cattle management and milk production. Testing of somatic cell count (SCC) with DCC (DeLaval Cell Counter) from herd milk samples (n=56) and observation of housing and animal body condition on-farm was also made.

Ethanol produced from lignocellulosic biomass has the potential to become a promisingalternative to gasoline. In this work the simultaneous saccharification and fermentation (SSF)technology was applied for ethanol production from hardwood with focus on cell growth,ethanol production and contamination.The SSF was performed at PH 5.5 and 35°C for different suspended solid concentrations(8%, 10% and 12%) of pretreated birch slurry which contained 16 % total suspended solids.Two different hexose fermenting yeast strain (Ethanol Red) and pentose fermenting yeaststrain were used.Quantifying the concentration of chemical components and metabolites in the fermentationmedium demonstrated that glucose and xylose are the major fermentable sugars in the slurry.The higher load of slurry (12%) represents a higher content of carbohydrates and potentiallyhigher end concentration of ethanol. Moreover, more lactic acid is produced with the lowerload of slurry (8 % or 10 %), presumably due to a result of a less inhibitory environment forbacterial growth. In this context, acetic acid sticks out as the most important inhibitor withconcentrations of 15.2 and 12.5 and 9.7 g/l respectively in the 12 %, 10 % and 8 % (ofsuspended solids) trials. Using pentose fermenting yeast may lead to higher ethanolproduction, lower xylose uptake and lower lactic acid formation.

Determination of serum thymidine kinase 1 (TK-1) activity is used as a tumour marker in both human and veterinary medicine. TK-1 is an intracellular enzyme involved in a salvage pathway of DNA synthesis. The expression of TK-1 is cell cycle dependent and the activity increases markedly after the G1 to S transition in the cell cycle and then declines rapidly in G2. The pronounced proliferative activity in tumour cells result in a higher TK-1 enzyme activity within the cell. The fact that the increased TK-1 activity can be measured in serum makes TK-1 a useful marker in diagnostics of neoplastic diseases and it provides information regarding prognosis and treatment effectiveness. In human medicine a radioactive based test is used to determine the TK-1 serum activity.

Increased consciousness about environmental and sustainable issues has led to consumers and companies who are more in favor of reuse and recycle. Even though this is a fact, people today are buying new things more often compared to before. For instance for a mobile phone the functionality lifecycle is eight years which can be related to the economic lifecycle today which is 1.5 years compared to 3 years in 1995. Only 1-2 percent of the mobile phones thrown away in Sweden today are handed in for recycling. The purpose of this study is to investigate whether an international company within the recycling and reuse of mobile phone industry has a market in Sweden.

The objective of this study has been to examine which significant values are of importance in the telecom sector today, and in a continuing perspective yet to be. To ensure the factors of relevance in the study, we have identified three purposes: - Our first purpose is to clarify the eminent values in the competitive market of the mobile phone operators today, and the reason for its importance.- The second purpose is to understand the function of the brand regarding to value creation and loyalty in the sector of mobile phone operators.- The third purpose is to explore the potential approaches for mobile phone operators to increase value.In this study, our approach has been a qualitative methodology by conducting seven interviews with respondents related to the telecommunication sector.As a result of the process, we find the need to divide the concept of value into two dimensions, which we entitle organizational related values and customer related values. We have found the values related to the characteristics of the market in aspects of its infrastructural features but also in regards to the homogenous core service.Our findings in the empirical data with support to our theoretical frame of reference, is the current highly important organizational related value of constant high result quality (well-functional technological aspect), and the value enhancer by a low price; the strategy of bundling also results in higher value. This result in customer related values that perceives as safeness, availableness and convenience. The brands are a significant source of customer related values with identity value, image value and assuredness. A continued advancement in process quality and individualization will imply greater customer related values of closeness, freedom and flexibility. .

In today?s society climate change has grown to one of the largest global issues. To reduce emissions of greenhouse gases, large changes of the energy system are required. An increasingly popular solution is investments in local production of renewable energy resources like wind, solar or geothermal power. The following report examines the possibility for implementing a solar power plant on the new hospital Nya Karolinska Solna. Two different technologies for extraction of solar energy has been studied, solar thermal collectors  to meet the need of heating and solar cells to satisfy the need for electricity linked to operating the hospital building.

Evaporation is a part of the chemical recovery department at wood pulping mills. The purpose of evaporation is to remove water from the black liquor, which makes the liquor combustible. The process is very energy demanding, and having a well-functioning model of the system is of greatest interest. The model can then be used to study energy consumption and to test different control strategies. Two models have been developed during the master thesis.

Nötcenter Viken is a Swedish nucleus herd for dairy cattle. The goal for the farm is to select suitable mothers for the next top generation of bulls. Today the selection for udder health is based on a pedigree index and phenotype data. The pedigree index is based on the cow?s father and grandfathers breeding values.

Fler människor börjar använda olika mobila operativsystem i mobiltelefonen samt appar som enbart fungerar på plattformen. Det leder till att app-leverantörer måste utveckla varje app till varje specifik plattform vilket är dyrt. De vanligaste operativsystemen som används idag är Android samt IOS men hösten 2012 släpptes Windows Phone 8 vilket innebär ytterligare en plattform att ta hänsyn till. I sammarbete med företaget Sogeti Jönköping fokuserar studien på att hitta en metod som gör det möjligt att migrera appar mellan plattformar och till Windows Phone 8. Studien innefattar också att identifiera för och nackdelar med olika app-typer genom en litteraturstudie och intervjuer samt att testa om migrationsmetoderna fungerar praktisk genom en implementation.Resultatet har påvisat att det finns två stycken olika metoder för migration vilket är PhoneGap och Xamarin.

Fler människor börjar använda olika mobila operativsystem i mobiltelefonen samt appar som enbart fungerar på plattformen. Det leder till att app-leverantörer måste utveckla varje app till varje specifik plattform vilket är dyrt. De vanligaste operativsystemen som används idag är Android samt IOS men hösten 2012 släpptes Windows Phone 8 vilket innebär ytterligare en plattform att ta hänsyn till. I sammarbete med företaget Sogeti Jönköping fokuserar studien på att hitta en metod som gör det möjligt att migrera appar mellan plattformar och till Windows Phone 8. Studien innefattar också att identifiera för och nackdelar med olika app-typer genom en litteraturstudie och intervjuer samt att testa om migrationsmetoderna fungerar praktisk genom en implementation.Resultatet har påvisat att det finns två stycken olika metoder för migration vilket är PhoneGap och Xamarin.

Uppdragsgivare för detta examensarbete är Länsstyrelsen Dalarna. Målet är att undersöka hur lämplig tekniken med CBS (Cell Broadcast Service) baserade textmeddelande är vid viktigt meddelande till allmänheten (VMA-larm). En tjänst som utnyttjar CBS-tekniken kan rädda liv om allmänheten i ett tidigt skede får reda på vad som hänt och vart de ska bege sig.Rapporten visar på hur man kan använda SMS då en krissituation uppstår. Med hjälp av CBS-tekniken skickas meddelandet till specifikt valda basstationer och alla mobiltelefoner som är uppkopplade mot den. Det positiva är att bara de som behöver få veta vad som hänt får meddelandet.Enligt specifikationerna (ETSI) på GSM och 3G-näten i Sverige finns det stöd för utskick av CBS-meddelanden inbyggt i de befintliga näten.

Purpose/Aim: The purpose of this thesis is to study the interaction between seller and buyer in a mobile phone store. The specific research questions under investigation are:Are there any underlying factors behind mobile purchase, and if so, what are they? Are mobile purchases driven by lifestyle factors and do customers try and create identities when purchasing new mobile phones?Material/Method: The study employs an ethnographic method when trying to answer the posed research questions. I have under a period of two weeks studied the seller-buyer interaction in a mobile phone store. In order to further increase the accuracy of my findings, I have complemented my observations with a sample of buyer and seller interviews.Main results: The observations and interviews were initially studied in isolation, resulting in different themes.

Fler människor börjar använda olika mobila operativsystem i mobiltelefonen samt appar som enbart fungerar på plattformen. Det leder till att app-leverantörer måste utveckla varje app till varje specifik plattform vilket är dyrt. De vanligaste operativsystemen som används idag är Android samt IOS men hösten 2012 släpptes Windows Phone 8 vilket innebär ytterligare en plattform att ta hänsyn till. I sammarbete med företaget Sogeti Jönköping fokuserar studien på att hitta en metod som gör det möjligt att migrera appar mellan plattformar och till Windows Phone 8. Studien innefattar också att identifiera för och nackdelar med olika app-typer genom en litteraturstudie och intervjuer samt att testa om migrationsmetoderna fungerar praktisk genom en implementation.Resultatet har påvisat att det finns två stycken olika metoder för migration vilket är PhoneGap och Xamarin.