At SLU, Divison of Reproduction, Dept of Clinical Science, there is an ongoing project on the development and evaluation of new methods for the assessment of stallion semen quality. This EEF-study, which is a part of that larger project, has focused on the effects on motility and fertility of stallion semen of two different extenders and selection. Both an objective method and a subjective method for analysis have been used and compared in the study. The increase in the use of artificial insemination with cooled semen in Swedish horse breeding over the last decade is thought to play a part in declining foaling rates (Kareskoski et al, 2006, Kuisma et al, 2006). This calls for better evaluation methods of semen quality since cooling and transportation put higher demands on semen quality in order to maintain fertility (Aurich, 2005).

AbstractThe purpose of this bachelor thesis is to describe the use of artificial insemination (AI) in sheep and discuss the possibilities for AI in Sweden. Male and female fertility, heat detection, semen handling and insemination techniques in sheep are described. Advantages and disadvantages of AI are discussed. In order to achieve genetic progress it is important with well planned and implemented breeding programs. The Swedish Sheep Recording Scheme and computer software from Elitlamm are the basis for sheep breeding in Sweden.

Uganda is one country with fastest growing populations in the world and with more than 25% of the population living in poverty. There is a rapid rural urban migration with increasing demands for food for low income earners especially in these areas. One way to mitigate food insecurity is to increase milk and meat production efficiency, and thus reproductive performance of cows is crucial for good production. A well-documented strategy for improving dairy cow productivity through faster genetic improvement is to breed dairy cows using proven semen via artificial insemination (AI). AI has been used in Uganda for over 60 years but only less than 10 %, a small population of the country?s herd has been bred that way. The aims were to study knowledge, attitude and practices factors influencing cow fertility results on dairy farms around Kampala using AI and to propose ways of promoting increased use of AI in Uganda.

Canine herpes virus 1 (CHV-1) är en vitt utbredd smittsam patogen. Viruset tycks verka enzootiskt i hundpopulationer och ger upphov till en livslång latens hos infekterade djur. CHV-1 kan sedan reaktiveras vid exempelvis stress, som vid dräktighet. Hos vuxna hundar får infektionen ofta ett subkliniskt uttryck, men kan också ge upphov till respiratoriska symtom samt genitala blåsor och ögoninfektioner. Dräktiga tikar kan drabbas av fertilitetsproblem, med mumifikation, resorption, dödfödsel samt abort av foster.

The prime aim of this thesis was to investigate whether high or low iodine intake during certain periods of life was, or was not a strong risk factor for the development of canine lymphocytic thyroiditis (CLT). And secondly, if a high or low selenium intake in a combination with high or low iodine intake also may have an influence on that risk factor. To investigate this, a dietary questionnaire was sent out to dog owners. The dogs were selected from an ongoing genetic study of CLT. The questions investigated brand and type of feed during different life stages (puppy, junior and adult) and also if there were any use of dietary supplements.

The aim of the study was to examine the retinal ON- and OFF-pathways of the canine electroretinogram (ERG). The ON- and OFF-pathways are used to distinguish objects from their backgrounds. A dark object is discernible from a lighter background using the OFFpathway and the ON-pathway is used to descry a light object upon a darker background. This study shows that the dog ERG has both ON- and OFF-responses and that these responses can be separated by prolonging the flash duration over 50 ms. The dog OFF-response waveform was more similar to the cat OFF-response than the human OFF-response (Frishman, 2006; Zrenner and Gouras, 1979). In this study six female three-year-old Beagle dogs were included. Both background and flash stimuli were provided by LEDs (light emitting diodes) with a wavelength of 470 nm, a wavelength that is absorbed almost as effectively by the canine M/L-cones as by the S-cones. These stimuli gave ERGs with both ON- and OFF-responses.

The use of artificial insemination (A.I.) as an alternative to normal mating in dog breeding in Sweden are controlled by regulations from the Swedish Board of Agriculture and by ethical rules assigned by the Swedish Kennel Club. Only veterinarians, who have a specific certificate and are specially trained and educated, are allowed to perform inseminations in dogs. Each artificial insemination must be documented and reported to the Swedish Kennel Club (SKK) which, on the behalf of the Swedish Board of Agriculture, yearly presents statistics concerning the use of A.I. in Sweden. The objective of this study was to illustrate the use of artificial insemination in dog breeding in Sweden for the last decade, using the yearly reports from the Kennel Club during the period of 1995 to 2004.

Oxytocin is a hormone which plays a crucial role in many reproductive and behavioural functions. It affects many organs and the classical peripheral targets are the mammary glands during lactation and uterus during labour. Oxytocin receptors have recently been described in a variety of normal tissues and primary cell cultures, but also in neoplastic tissues and established neoplastic cell lines, as breast cancer cell lines. The signalling pathways and biological effects of the oxytocin/oxytocin receptor system seem to depend on species, type of tissue, physiological versus neoplastic state and receptor location within the cell membrane.The aim of this project was to find out if the cell proliferation in the canine mammary carcinoma cell line CMT-U27 was affected upon stimulation of oxytocin, and to investigate the presence of oxytocin receptors in these cells. The cell proliferation was examined by using an ELISA-kit, where the absorbance measured is relative to the amount of living cells.

The purpose of this thesis was to analyze conception and foaling results of Swedish warmblood and Standardbred trotters using breeding statistics during the years 2000-2006. Key issues in this thesis were: Have the conception and foaling results of stallions changed during the period 2000-2006? What is the effect of the use of various breeding methods? Is testicle size correlated with conception and foaling results? How important are age, breed and level of inbreeding of the stallion for conception and foaling results? Breeding statistics were available for 537 Swedish Warmblood stallions and 305 Standardbred trotter stallions. To be included in the analyses, a stallion must have mated at least ten mares in total over the period 2000-2006. This criterion was met by 313 Swedish Warmblood stallions and 272 Standardbred trotter stallions.

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Fryst eller kyld sperma används ofta vid artificiell insemination på hund. Skador kan uppstå på spermierna under frysningsprocessen. För att spermierna ska bibehålla sin viabilitet och undvika skador tillsätts spädningsvätskor som bland annat innehåller sockerarter, antibiotika, glycerol och äggula. Äggula skyddar spermierna mot frysskador och bidrar till en ökad spermieöverlevnad, dock är mekanismen bakom detta ej helt klarlagd. Att äggula ingår i spädningsvätskan kan medföra en risk för mikrobiell kontamination och innebära ett problem vid internationell handel med sperma.

The development of quantitative, real-time PCR (qPCR) combined with the mapping of the canine genome opens new possibilities in veterinary medicine. This method provides a quick and accurate quantification of the expression of a specific gene at a given point in time and thereby also information of how the gene expression for a certain protein is influenced by various conditions and diseases. One possible area of application is identifying bio markers for cancer. Recently the protein serglycin (the core protein of an intracellular proteoglycan) was found to act as a selective marker for the disease acute myeloid leukemia in humans (Niemann et al, 2007). Serglycin is produced by most hematopoietic cells, although mast cells account for the largest amount of serglycin.

Full-field flash electroretinography is an electrodiagnostic method used to examine the function of retinal cells. Light stimulation of the eye elicits electrical potentials in the retina. By aid of a corneal electrode and a reference electrode close to the back of the eye, the electrical potentials can be recorded and presented as an electroretinogram (ERG). ERGs driven by mainly one type of cones can be used to examine the function of a single cone class. In human beings, studies have shown the cone class sensitive to light of short wavelengths, the S-cones, to be more vulnerable to acquired damage than the other cone classes (Daley et al., 1987; Gouras et al., 1993; Greenstein et al., 1989). Dogs have two cone classes, S-cones, and M/L-cones (most sensitive to medium to long wavelengths).

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This article presents a retrospective case study combined with a review of the current literature concerning canine gestational diabetes. A total of 14 cases have been collected from six different clinics in Sweden. There are few previously published reports on canine gestational diabetes. Gestational diabetes is a disease that rarely occurs in pregnant bitches. The condition is due to the rise of progesterone that occurs during the estrus cycle.