The cell cycle is the time a cell spends between two cell divisions. The cell cycle includes several parallel processes, all of which must be completed before a cell is mature for dividing. In the first place, all subcomponents (RNA, protein and membrane lipids) need to double in quantity and this occurs continuously through the cell cycle. Furthermore, the genome and some chromosomal proteins must double and this take place during a limited interval in the middle of the cell cycle (S-phase). On either side of the S-phase are two "gaps" which is called G1 and G2. When the cell has passed a point in the G-phase they are irreversibly programmed to progress through the remaining of the cell cycle and will undergo the next cell division. When a cell have passed a point in the G1 phase, approximately four hours after mitosis, a normal cell is in a state of indecision.

I denna studie har förekomst av progesteronreceptorer i olika typer av primära juvertumörer undersökts med hjälp av immunohistokemi. Dessutom har tumörceller från carcinomcellinjen CMT-U27 odlats i olika cellodlingsmedium och exponerats för olika testsubstanser som binder till progesteronreceptorn (progesteron och RU486/mifepristone). Cellernas receptoruttryck och progesteroninnehåll utvärderades med hjälp av ELISA och tumörcellernas viabilitet analyserades med Colorimetric Cell Viability Test. Av resultaten framgår att samtliga undersökta primära juvertumörer uttryckte progesteronreceptorer. De odlade cellerna hade högre cellviabilitet när de odlades utan fetalt bovint serum (FBS). Efter exponering med progesteron minskade tumörcellernas uttryck av progesteronreceptorer, vilket hämmades av mifepristone.

The goal of this paper is to find out how solar cells can improve energy efficiency on-board M/S Sydfart. The paper is based on a number of energy measurements on board the M/S Sydfart. The solar surface is calculated by measuring the available space for installing solar panels. Global radiation data is taken from STRÅNG's database, the data is then used to calculate the theoretical power produced by the solar cells. Energy efficiency is analysed in two ways.

Solar energy is the one most talked about among energy sources due to its huge potential amongst renewable energy technologies. A greater use of the solar energy may be the solution to today's climate threats. Normally there are two different types of solar technologies, solar cells and solar thermal collectors. Solar cells convert solar energy in to electricity whilst solar thermal collectors convert solar energy into heat, which can be used to heat hot water for households. This goal of this study has been to investigate the possibility of implementing solar energy into the energy system of Kista Galleria, one of Sweden's foremost venues.

The problem of finding targeted medicine is a central problem in chemotherapy. From this point of view the ubiquitin-proteasome system is a highly promising object in the pharmaceutical approach. Proteasome plays a critical role in cellular protein degradation, cell cycle and apoptosis regulation.Proteasome inhibitors are substances blocking the actions of proteasome. Cancer cells are more sensitive to inhibition of the ubiquitin-proteasome system than normal cells. Therefore proteasome inhibitors have the potential to be successfully used in the cancer treatment.The study aimed to test various substances to identify possible proteasome inhibitors with the IncuCyteTM FLR image system and fluorometric microculture cytotoxicity assay.

Serum thymidine kinase 1 (TK1) activity is used as a tumor marker in disease monitoring in veterinary and human medicine. TK1, an intracellular enzyme, is involved in a salvage pathway of DNA precursor synthesis. TK1 is used in DNA precursor production by catalyzing the transfer of the gamma-phosphate-group from a phosphate-donor to the 5?- hydroxyl-group of thymidine forming thymidine-monophosphate. Nucleoside monophosphosphates are finally converted into thymidine-triphosphates. TK1 activity significantly rises in the G1 and the S phase of the cell cycle.

Scrapie is an infectious neurologic disease in sheep caused by prions, corresponding to transmissible spongiform encephalopathies (TSEs) in other species. The prion is presumably constituted of PrPSc, the misfolded form of the normal endogenous prion protein, PrPC, which is found in practically all cells in the body. PrPSc can induce a conformational change in PrPC and misfold it as an imprint of itself; this is how prions amplify and spread. The process of conformational change is poorly understood and there might exist intermediate forms between PrPC and PrPSc. Bioassay using mice has traditionally been the golden standard in prion studies but is limited by cost and time.

Cyanobacteria belong to the oldest organisms of our planet. They use photosynthesis to produce ATP and gain biomass from carbon dioxide. The cyanobacteria Nostoc punctiforme is a filamentous bacterium that consists of two different types of cells, vegetative cells and heterocysts. The type of cell it differentiates into depends on the media they grow in. In an ammonium-rich medium, the N.punctiforme consists of vegetative cells that differentiate into heterocysts when in the medium is changed to a low-concentration ammonium medium.

This report deals with the design of a controller for SmartMedia? flash memory cards, based on a hardware description found in the SmartMedia? Interface Library - SMIL. The design was made on logic gate level, using standard cells in OrCAD Capture. After simulation of the design in PSpice A/D, it was exported as an EDIF netlist, which was used to make a chip layout in L-Edit, a layout tool for making integrated circuits. The layout was made using a method called Standard Place and Route - SPR, where the layout tool places standard cells from a library and connects them according to the EDIF netlist.

Canine herpes virus 1 (CHV-1) är en vitt utbredd smittsam patogen. Viruset tycks verka enzootiskt i hundpopulationer och ger upphov till en livslång latens hos infekterade djur. CHV-1 kan sedan reaktiveras vid exempelvis stress, som vid dräktighet. Hos vuxna hundar får infektionen ofta ett subkliniskt uttryck, men kan också ge upphov till respiratoriska symtom samt genitala blåsor och ögoninfektioner. Dräktiga tikar kan drabbas av fertilitetsproblem, med mumifikation, resorption, dödfödsel samt abort av foster.

The prime aim of this thesis was to investigate whether high or low iodine intake during certain periods of life was, or was not a strong risk factor for the development of canine lymphocytic thyroiditis (CLT). And secondly, if a high or low selenium intake in a combination with high or low iodine intake also may have an influence on that risk factor. To investigate this, a dietary questionnaire was sent out to dog owners. The dogs were selected from an ongoing genetic study of CLT. The questions investigated brand and type of feed during different life stages (puppy, junior and adult) and also if there were any use of dietary supplements.

Heifer?s growth may be divided into three main phases: calf period, puberty and pregnancy. When the heifers are about three months old a critical period begins. This period ends when the heifer reach puberty and have her first oestrus at around 9-12 months of age. In the critical period the mammary gland starts to grow at a fast rate and Insulin-like growth factor-1 (IGF-1) transport the growth signals to the mammary glands.

The aim of the study was to examine the retinal ON- and OFF-pathways of the canine electroretinogram (ERG). The ON- and OFF-pathways are used to distinguish objects from their backgrounds. A dark object is discernible from a lighter background using the OFFpathway and the ON-pathway is used to descry a light object upon a darker background. This study shows that the dog ERG has both ON- and OFF-responses and that these responses can be separated by prolonging the flash duration over 50 ms. The dog OFF-response waveform was more similar to the cat OFF-response than the human OFF-response (Frishman, 2006; Zrenner and Gouras, 1979). In this study six female three-year-old Beagle dogs were included. Both background and flash stimuli were provided by LEDs (light emitting diodes) with a wavelength of 470 nm, a wavelength that is absorbed almost as effectively by the canine M/L-cones as by the S-cones. These stimuli gave ERGs with both ON- and OFF-responses.

Mammary gland neoplasia is one of the most common diseases in the bitch and the most common neoplastic disease. More than 13 % of the female dog population in Sweden suffers from mammary tumors during their lifetime. The disease can affect bitches in all ages but most often middle aged and older dogs. Some breeds such as Poodle and English Setter are predisposed for the disease. The aim of this study was to evaluate if it is possible to improve the post surgical healing process by using a pressure pad.

The purpose of the project is to determine the impact of solar cells and electrical vehicles on the future electricity grid and distribution network. Future electricity grids will be affected differently than it does today. Therefore, it is important to determine the impacts so that the current electricity distribution system can be developed and redesign to achieve the future demand. In Sweden, government has changed rules and laws in order to make it easier for private sector to invest in renewable energy sources. Our project focused on the impact of the solar cells and electric vehicles on the low voltage electricity distribution.