The development of quantitative, real-time PCR (qPCR) combined with the mapping of the canine genome opens new possibilities in veterinary medicine. This method provides a quick and accurate quantification of the expression of a specific gene at a given point in time and thereby also information of how the gene expression for a certain protein is influenced by various conditions and diseases. One possible area of application is identifying bio markers for cancer. Recently the protein serglycin (the core protein of an intracellular proteoglycan) was found to act as a selective marker for the disease acute myeloid leukemia in humans (Niemann et al, 2007). Serglycin is produced by most hematopoietic cells, although mast cells account for the largest amount of serglycin.

Full-field flash electroretinography is an electrodiagnostic method used to examine the function of retinal cells. Light stimulation of the eye elicits electrical potentials in the retina. By aid of a corneal electrode and a reference electrode close to the back of the eye, the electrical potentials can be recorded and presented as an electroretinogram (ERG). ERGs driven by mainly one type of cones can be used to examine the function of a single cone class. In human beings, studies have shown the cone class sensitive to light of short wavelengths, the S-cones, to be more vulnerable to acquired damage than the other cone classes (Daley et al., 1987; Gouras et al., 1993; Greenstein et al., 1989). Dogs have two cone classes, S-cones, and M/L-cones (most sensitive to medium to long wavelengths).

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This article presents a retrospective case study combined with a review of the current literature concerning canine gestational diabetes. A total of 14 cases have been collected from six different clinics in Sweden. There are few previously published reports on canine gestational diabetes. Gestational diabetes is a disease that rarely occurs in pregnant bitches. The condition is due to the rise of progesterone that occurs during the estrus cycle.

Thymidine kinase 1 (TK1) is an intracellular enzyme involved in ?the salvage pathway? where the uncomplete parts of DNA are transformed into DNA precursors. The expression of TK1 is cell cykle specific, with the highest level during S-phase when DNA-synthesis is most active. One kind of TK1 is present in serum and an increase in TK1 activity in serum is due to either the level of DNA synthesis in the body or the number of cells dying in a state of replication. This can be used and is used as a marker for prognosis and evaluation of treatment in human patients with leukemia and lymphoma.

The aim of this study was to describe how body language of humans affects the domestic dog (Canis familiaris) and how it can be implanted in the stressed situation that occur at an animal hospital. The subject is brought to notice because the author has experienced that dogs can get more stressed when a stressed and nervous owner is attending. Humans affect each other through posture, facial expression, voice and movement, and are often driven by their emotions, which can differentiate the verbal communication from the non-verbal communication. This occurs both consciously and unconsciously. Every human uses body language but not everyone knows how it is used or how to understand it.

This report describes the nutritional requirements of the dog and cat. You are also able to learn about the most common diseases in the dog and cat where food has an important role in both prevention and recovery of these diseases. We describe FLUTD, canine urolithiasis, heart- and kidney disease, gastrointestinal diseases and obesity. You have to know the right balance between different nutrients to decrease the stress on the body and to prevent the advancement of the diseases. Finally we discuss the differences between three manufacturers of veterinary prescribed diets and two commercial pet foods..

Electroretinography is an examination method where the function of the retina is objectively assessed. In 2002 the European College of Veterinary Ophthalmology (ECVO) published a proposition to a standard on how to perform ERG examinations, to facilitate comparison of results and to make it possible to repeat examinations. The purpose of this study was to validate parts of the canine ERG protocol suggested by the ECVO. Our principal aim was to study if there is any interference at the recommended stimulus intervals for dark-adapted rod- and combined rod-/cone responses. The results show that there is an effect on the a- and b-wave amplitudes when different stimulus intervals are employed. When all examined intervals are pooled, there is an increase in the median b-wave to each consecutive dim, white (rod) stimulus in a series of four.

Rabies kills about 55 000 people every year and more than 90% of infected humans are considered getting the disease from dog-bites. To control the disease and eventually eliminate human rabies, the most efficient and economic method is to keep the dog population vaccinated. The objective of this thesis was to evaluate the situation of canine rabies in two urban areas in Lilongwe, Malawi, where canine rabies is enzootic. In each area 200 household-interviews were conducted in September and October 2013. Focal points were awareness of the disease, human incidence as well as vaccination coverage in the dog population.

Abort som orsakas av virus förekommer hos hästar över hela världen, och skapar problem för uppfödare. De främsta bakomliggande virusen är ekvint herpesvirus 1 (EHV-1) och ekvint arterit virus (EAV). Fall inträffar tillföljd av båda virusen varje år i Sverige, och framförallt EHV-1 har varit föremål för mycket forskning för att hitta ett skyddande vaccin. Det finns i Sverige ett avdödat vaccin mot EHV-1 upptaget i FASS. EAV däremot vaccineras det i princip inte mot i landet, men utomlands finns och används i vissa länder både avdödade och attenuerade levande vaccin.

The dog is unique in the way that the adult weight can vary 100 folds between the different breeds. Despite this, most of the breeds reach adult size before two years of age. For the large-breed dogs this means a very rapid growth when some of them can have a weight gain of up to 150 times their birth weight. The most rapid growth occurs when the puppies are between three to six months old and that kind of rapid growth during such a short period of time makes these large-breed puppies very sensitive to deficits and excesses of energy, nutrients and minerals. Some examples can be excess of energy, excess or deficit of minerals such as calcium and phosphorus or deficits of protein. The rapid growth can also make the large-breed puppies predisposed to some diseases affecting the skeletal and joints.

Renal dysplasia is a developmental anomaly of the kidneys which is considered to be the primary lesion in juvenile progressive nephropathy, a condition leading to chronic renal failure in young dogs. It is defined as disorganised development of the renal parenchyma due to abnormal differentiation, and pathological lesions include persistent foetal structures, dysplastic tubules and cartilaginous and osseus metaplasia. The aetiology of renal dysplasia in dogs is still unknown, but possible causes are infection with Canine herpesvirus, teratogenic substances or intrauterine ureteral obstruction. In some breeds renal dysplasia is a familial or inherited disease. Clinical signs of chronic renal failure, such as polyuria, polydipsia, vomiting, weight loss, anorexia, lethargy and anemia, usually appear before the age of 2 years.

A lot of various substances can be detected by dogs, as oestrous in cows, endangered species or non-biological substances like accelerants, narcotics, explosives, mines and pollutions and biological substances like identity (suspects), cancer (breast, lung, colorectal and bladder cancer), human remains (cadaver), bacteria and mould in buildings. The dogs are trained using non-scientific methods and not much research in detection training exists. This is a review of different types of dog detection, with focus on new research..

Measurement of plasma concentrations of natriuretic peptides have been suggested to be of diagnostic and prognostic value in canine cardiac disease. In several previous canine studies, however, a considerable overlap between investigated groups has been identified. A reliable natriuretic peptide test for use in clinical practice requires establishment of reference values for natriuretic peptides in healthy dogs and cut-off values for subclinical disease as well as for clinical signs of congestive heart failure. Numerous physiological, pathological and pharmacological factors, aside heart function, may influence concentration of natriuretic peptides. A potential effect of dog breed on natriuretic peptide concentration has been suggested but has, to our knowledge, not previously been specifically studied.

There are about 700 000 dogs in Sweden. Roughly 70 % of the dogs are purebred and registered in the database at the Swedish Kennel Club (SKC). The database includes records of birth date, pedigree, offspring and results from e.g. veterinary examinations related to genetic health programs.Every year approximately 10% of the dogs die; most are euthanized by a veterinarian. Dog mortality data is stored as digital medical records at animal clinics and in insurance databases.Dog owners tend to register their puppy in the SKC, but they rarely report that the dog has passed away.