Syftet med arbetet var att utveckla en metod för att rena fram cytokrom c-Id1 ur periplasma från Ideonella dechloratans. Inledningsvis undersöktes proteinets stabilitet vid låga pH. Flera olika kromatografiska metoder provades under arbetets gång. Till de inledande experimenten användes flow-through fraktioner från rening av annat protein som startmaterial. Senare försök gjordes på periplasma från I.

Denna studie undersöker hur stressfaktorer påverkar kroppen vid dykning i gruva. Undersökningen genomfördes under en helgs intensivt dykande, för att bestämma lungkapacitet, pulsnivå samt eventuell vätske- och viktförlust hos dykarna. Testerna bestod av vägning, spirometritester och pulsmätningar. Testpersonerna dök mellan ett till tre dyk under helgen. Två av det totala antalet dyk var på 74 meters djup, resterande dyk genomfördes på 36 meters djup.

En god nutritionsstatus är av betydelse för en optimal sårläkning. Syftet med denna studie var att beskriva sambandet mellan nutrition och sårläkning ur ett omvårdnadsperspektiv. Studien gjordes som en systematisk litteraturstudie och baserades på 16 vetenskapliga artiklar. I dessa framkom nutritionsstatusens samband med sårläkning och vad sjuksköterskan kan påverka genom sina omvårdnadsåtgärder. Det kom även fram att energibehovet ökade när patienterna hade svårläkta sår.

The purpose of the study was to examine what methods and strategies individuals use to maintain their weight loss. Another aim was to explore which factors can complicate or simplify weight loss maintenance. Fifty-four individuals who had maintained their weight loss more than a year, participated by answering a questionnaire. The questionnaire was constructed and distributed through the internet. The answers were registered in the program google docs.  The participants mentioned successful methods and strategies, such as exercising regularly, the majority exercised 30 minutes at least five times per week.

The quality of bread wheat is a large subject to study. The quality rests on two legs. The first one is the inheritance of specific characters, which can be influenced through plant breeding and type of cultivar. The other one is the environmental influences, such as crop rotation, nutrition, harvest and storage. The latter factors are possibly to affect through cultivation practices, while other factors are not possible to influence on for the grower, such as seasonal changes of weather and the soil content.

Genetic defects are caused by mutations in major genes where the gene?s protein product has a large impact on the physiology of the animal. The synthesis of the protein can be altered by a change in the nucleotide sequence, which can lead to malformation and in many cases death.One of the main reasons of increase in many genetic defects is the use of few bulls in breeding programmes, causing a reduction of the genetic variation. Genetic defects cause suffering for the animal and influences the production by, for example, increased costs due to misscarriages, lost milk production and expenditure for medical treatment. Bovine Leukocyte Adhesion Deficiency (BLAD) and Complex Vertebral Malformation (CVM) are two genetic defects that were widespread before the causative mutation was discovered.

Nutrition är en viktig och central del av människans liv. När man inte får i sig tillräcklig mängd näring kan det få allvarliga följder så som malnutrition, ett tillstånd som ofta sätts i samband med trycksår. Trycksår orsakar lidande både fysiskt och psykiskt för de som drabbas och har därigenom påverkan på deras livskvalitet. Syftet med studien var därför att söka evidens för betydelsen av näringstillskott för läkningen av trycksår. Evidensstudien baserades på nio vetenskapliga artiklar.

Haptoglobin is an acute phase protein with important biological role because of its capacity to bind to haemoglobin. Haptoglobin exists in three major genetic polymorphism types: Hp1-1, Hp2-1 and Hp2-2, the distribution of which has been associated with abdominal aortic aneurysm (AAA), an asymptomatic aortic disease common among men older than 65 years.    Five different electrophoretic methods were tested according to their ability to separate the haptoglobin phenotypes. The detection was based on a produced hemolysate of blood in which haemoglobin binds to haptoglobin thereby forming a complex that can be detected by specific haemoglobin staining using TMB-dihydrochloride and hydro peroxide as substrate resulting in an azure-green color of the bands. Samples from 15 patients who had suffered surgery for not broken AAA, that is more than5.0 cmaortic diameter, and 15 samples from matched controls were analyzed.    Among the five tested electrophoretic methods best migration and separation was seen on the pre-cast agarosgel Hydragel HR on the instrument Hydrasys. The other four methods gave less successful results.

The process of drug discovery is very slow and expensive. There is a need for reliable in silico methods; however the performance of these methods differs.This work presents a targeted study on how the drug discovery methods used in OpenEye?s tools ROCS, EON and FRED perform on targets with small ligands. It was examined if 12 compounds (markers) somewhat similar to AMP could be detected by ROCS in a random data set comprised of 1000 compounds. It was also examined if EON could find any electrostatic similarities between the queries and the markers.

Lao People?s Democratic Republic (PDR) is a country where the majority of the population works within the sector agriculture in one way or another. The number of goats are increasing and the animals can be an important income source for families. However, during the dry season, lack of feed is a great problem for the farmers. Fodder trees have shown great potential as dry season feeding, due to that the trees often hold green and fresh leaves a long time into the season, compared to grass that dry out quickly.

The objective for this master?s thesis was to test and evaluate two methods for determining the content of tannin in a pinebarkextract. The methods used at Södra for this previously have not been specific enough, only the amount of polyphenolics have been measured. One of these methods is a test based on the Stiasny test and it determines the extracts ability to form a gel with formaldehyde. When this test was carried out it showed that the extract did not contain much tannin.

SAMMANFATTNINGBakgrund Undernäring är vanligt förekommande bland äldre i Sverige varav en orsak är att de äter för få måltider och har dålig aptit. Det är därför viktigt att de äldre erbjuds tillräckligt många mål mat per dag och att maten är energi- och näringsriktig. Sjuka, undernärda äldre behöver ofta äta energi- och proteinrik kost för att uppnå sina behov. Det är viktigt att motverka och behandla undernäring då det kan medföra bland annat försämrad livskvalité. Med denna studie ville vi ta reda på och belysa hur de äldres näringsintag verkligen såg ut.Syfte Syftet med studien var att studera intag av energi, protein, järn, kalcium samt C och D-vitamin under två huvudmåltider, två dagar hos geriatriska patienter som åt E-kost.Metod Metoden som användes var en vägd kostregistrering samt fotografering.

In developing countries such as Vietnam the population consumes more animal products for example milk and meat than before which requires greater livestock production. Ruminants contributes to more methane emission which creates a dilemma between food production and its environmental impact. By feeding ruminants with nutritive crops which humans can not assimilate the animal performance will increase in terms of better growth and milk production without inpinging on food that can be consumed directly by humans. Increasing animal performance reduces methane emission in terms of amount of methane in kg-1 milk and meat which today is much greater in developing countries than developed countries. The aim of the study was to investigate tropical tannin-rich legumes for their potential as a feed supplement for ruminants made in vitro.

This paper concerns a comparative analysis between two pre-Roman burial ground and the cremated individuals buried there, Skogome cemetery in Bohuslän and Smörkullen cemetery in Östergötland. The comparative analysis consists of several parts that concern both cemeteries inner and outer burial customs, and the osteological analysis of a total of 18 cremated invidvidulas. By studying the different parts separately, it has been possible to identify similarities and differences between the two contemporary cemeteries. There are great similarities between the cemeteries, differences were mainly observed in the osteological material relating to the amount of bone in each burial. The smaller amounts of bone in the graves of Skogome also holds a higher degree of fragmentation, but can not be explained by a higher combustion rate than the skeletal material from Smörkullen.

Studien handlar om producenter och konsumenters medvetenhet om de utmaningar som finns g?llande en mer v?xtbaserad kost, med s?rskilt fokus p? konsumenters uppfattningar om n?ringsinneh?llet. Definitionen av konsumenter i denna uppsats ?r individer som konsumerar k?ttsubstitut utan n?got krav p? regelbundenhet. Producenter definieras som talespersoner f?r diverse f?retag av k?ttsubstitut. K?ttsubstitut n?mns i denna studie och definieras som texturerat v?xtbaserat protein och halvfabrikat.