The coproduct, dried distillers grains + solubles (DDGS) from ethanol production based on wheat contains much rumen degradable protein and fiber with low digestibility. To compliment this quality in an effective way a grass silage low in protein and much digestible fiber is needed. The aim of the study was to investigate the effects of adjusting the crude-protein concentration and fiber quality of the silage when feeding DDGS and of varying the inclusion level of DDGS, when feeding the adjusted grass silage, on intake, milk yield and composition, faecal traits and feed efficiency. Four different total mixed rations were compared. The normal grass-clover silage had a crude protein content of 17,8 % and an NDF content of 46,2 % of dry matter. The adapted grass silage had a crude protein content of 14,6 % and an NDF content of 52,9 % of dry matter. The diets were formulated to have similar nutritional content except for A + 4 which had a higher content of rumen degradable crude protein and a lower concentration of NDF.

The following project has been made in collaboration with, Denator AB, a biotechnology enterprise. One-dimensional electrophoreses have been used to analyze protein contents of tissue samples. Tissue samples have been either treated according to the company´s newly developed tissue treatment technique or not treated at all and comparisons between these samples have been made. In order to see differences in protein patterns between samples more clearly, a conversion method has been developed where electrophoresis gel visual patterns are used to produce curves, similar to densitometer curves.Denator AB would like to know if the proteins in their treated samples will change visibly over 48 h when incubated at room temperature. This has been investigated using the above mentioned method.

This bachelor-level thesis in historical osteology deals with several aspects of the life-cycle of an RomanIron Age farm in southern Scania. Excavated in the 1980's by Lars Larsson, the farm was located near aplace called Långåker in the Dybäck area, on a cape which stretched out into a minor bog. Our work concerns why the Iron Age people settled the area, how well fragmented bone survives in a bog, what they produced at the farm and how life on the farm finally ended. From the roughly 10 % of the total number of fragments we where able to identify we can conclude, that he species that are present of the farm areusual Iron Age species (dog, sheep/goat, cattle and swine). We also have a relitivly large number of wildanimals in our material (2%).

The aim in this thesis is to use carbon, nitrogen and sulfur isotope analysis on teeth and bone to track diet and mobility of the population as well as the individual level from the articulated and disarticulated individuals from the passage grave Frälsegården, situated in Gökhem parish in the region of Falbygden, western Sweden.50 human samples were analyzed from tooth, mandibula and femur, together with 11 faunal samples from different bone elements of local wild and domestic animals.The results fall well in line with earlier stable isotope work done on passage grave individuals from Falbygden in stable isotopes: A homogenous group with a diet which was clearly terrestrial and lived on a pretty high trophic level. It also seems that the analyzed individuals deliberately focused their food intake to the cultural and natural, megalith and limestone landscape of Falbygden, and not to its surroundings, even if the passage grave is situated in the outermost areas of the region.A small study compares the sulfur results to with earlier work done on strontium isotopes. The results show that the different methods might provide a deeper understanding in the interpretation of the methods, and therefore suggesting that further work should be done to compare the methods..

PSI-N is one of the subunits in eukaryotic Photosystem I (PSI) and is located on the lumenal side of the thylakoid membrane. It is known to interact in the electron transport chain between plastocyanin and PSI, but the mechanism behind the interaction is still unclear. To achieve a better understanding of PSI-N´s role in the photosynthesis it is necessary to develop a method for purification of PSI-N. The goal with this project was to design a plasmid that encodes a fusion protein containing PSI-N. With use of proteases the fusion protein can be cleaved into purified PSI-N.

PSI-N is one of the subunits in eukaryotic Photosystem I (PSI) and is located on the lumenal side of the thylakoid membrane. It is known to interact in the electron transport chain between plastocyanin and PSI, but the mechanism behind the interaction is still unclear. To achieve a better understanding of PSI-N´s role in the photosynthesis it is necessary to develop a method for purification of PSI-N. The goal with this project was to design a plasmid that encodes a fusion protein containing PSI-N. With use of proteases the fusion protein can be cleaved into purified PSI-N.

This is a survey of non-traumatic skeletal abnormalities of the domestic cat. The types of diseases that are presented are congenital, developmental, metabolic, degenerative, neoplastic, inflammatory, infectious and a few diseases of uncertain aetiology. Each disease, where possible, is described in aetiological, symptomatical and radiological aspect. In the cases where there are plausible treatments these are presented..

Sulforafan är en isothiocyanat-förening som finns i stora mängder i olika kålväxter. Sulforafan har setts ha en kemopreventiv inverkan bland annat genom att inducera fas II-enzymer styrda av transkriptionsfaktorn nuclear factor E2-related factor-2 (Nrf2). Nrf2 är mycket viktig i cellers skydd mor oxidativ- och elektrofil stress och inducerar uttrycket av många olika gener, bland annat olika tranportproteiner. Breast cancer resistance protein (BCRP) är en av transportörerna som tros induceras av Nrf2. BCRP finns i stora mängder i lakterande juvervävnad men också i många andra vävnader där den transporterar läkemedel ut ur cellerna.

In this study bone material from six stone ship settings and a total of seven deposits of bones from two sites on the island of Gotland have been analyzed. Four ship settings from the burial site at Gålrum in Alskog parish and two from Tängelgårda in Lärbro parish. The ships contained both cremated remains and inhumations.  Human remains were identified in five of the analyzed ship settings and a minimum of six individuals was identified in total.  Animals were found in three of the ship settings, where one of them contained only the burned remains of a dog. Two of the ships contained inhumations, one in Gålrum and one in Tängelgårda.

This paper deals with diet and gender in the early roman iron age in Sweden. Human bone and tooth material from the Iron Age cemetery at Bjärby in Kastlösa parish on the island of Öland have been analysed for stable carbon and nitrogen isotopes. 21 individuals were sampled. Where possible 4 samples were taken from each individual, from the first, second and third molar respectively and from one compact bone. By doing so it is possible, at least in theory, to trace intra-individual diets throughout life.

Arbetet beskriver en analys av innehållet av membranbundna och periplasmiska c-cytokromer hos perkloratodlade GR-1 och jämförelser med c-cytokrominnehållet hos Ideonella dechloratans och andra kända c-cytokromer, samt med genomet för Dechloromonas aromatica. Den jämförande studien av c-cytokromer gjordes med syftet att undersöka en hypotes om att bakterierna använder olika vägar för elektronöverföring till det periplasmiska enzymet (per)kloratreduktas. Cellmembran från GR-1 renframställdes genom ultracentrifugering och periplasma preparerades fram med hjälp av osmotisk chock. Fraktionerna analyserades sedan med SDS-PAGE och peptider med kovalent bundet hem (c-cytokromer) detekterades med hjälp av en specifik färgreaktion. Även Touchdown PCR med degenererade primrar genomfördes på isolerat DNA från GR-1 i ett försök att finna en gen kodande för ett NapC/NirT-liknande protein.

Bovin spongiform encephalopathy (BSE) är en sjukdom som drabbar nötkreatur, och varssmittämne är det ofullständigt utforskade prionproteinet. Detta protein finns i vår egen kropp,men då veckat till strukturen av en alfahelix istället för ett betaflak. När vi får i oss ettfelveckat protein verkar det som att en domino-effekt sker och alla normala proteiner avdenna sort veckar sig fel och ansamlas i centrala nervsystemet (CNS). Människor som ätersmittat kött kan drabbas av den humana formen, variant Creutzfeldt-Jakobs sjukdom (vCJD),som är en neurodegenerativ sjukdom med lång inkubationstid och som oavkortat leder tilldöden. I denna litteraturstudie görs ett försök att belysa dessa sjukdomar och problematikenrunt omkring, med betoning på BSE.

There is convincing research which shows that the enzyme alkaline phosphatase (ALP) has a central role in the mineralization of bone, more precisely that its catalytic activity is needed in the process. ALP is found on the surface of matrix vesicles where the mineral is formed. One theory about the function of the enzyme is that it binds to fibrous collagen in the bone and thereby incorporating the mineral into the bone. The purpose of this study is to establish whether ALP binds to collagen. If this is the case, more elaborate studies around this will be performed.

AbstractAn approaching overproduction of cereals and a future uncertain supply of non genetic modified vegetable protein feedstuffs have increased the interest in an expansion of the domestic production of protein feedstuffs in Norway. Besides rapeseed (Brassica campestris), peas (Pisum sativum) may be the most suitable crop for this purpose. Peas are characterized by having a relatively high content of crude protein, ranging between 20-26%, a high content of starch, 42-51%, and a low content of fat. In general, the energy value for peas is higher than for barley but lower than for rapeseed and soybean meal.Pea protein consists of albumins and globulins to 85-100%, which leads to the fact that a large part of the pea protein is soluble and degradable in the rumen. Starch in peas is on the other hand to a large extent resistant to rumen degradation compared to starch from other starch rich feedstuffs.

Working horses in developing countries are mostly used for transportation. They are often owned by people with limited economical resources and lack of knowledge about how to take care of the horses and due to this the horses often suffer from problems such as dehydration, poor body condition, lesions and parasitic infections. The aim of this master thesis was to investigate if free veterinary care improved the health and hydration status of working horses in Nicaragua and it was performed as a part of a project called ?Free veterinary assistance for working horses and training of their owners as veterinary promoters in the Pacific coast of Nicaragua?. Five parameters were used to investigate whether free care improved the health of working horses in six towns along the Pacific Coast of Nicaragua.