Tissue factor (TF), a 47 kDa glycoprotein, is the initiator of the extrinsic pathway of blood coagulation and consequently of the upmost importance when damage to blood vessel occurs. The source of TF in circulation has been investigated. However, the source of TF is still not clear. One theory is that platelets express and increases the expression of TF after stimulation and the aim of our report was to investigate whether platelets really are a source for TF in circulation.Using specific primers for TF mRNA, platelets in plasma from healthy volunteers and from patients suffering from cardiac infarction were analyzed by using real-time polymerase chain reaction (PCR). Gel electrophoresis was performed after amplification of TF mRNA to verify the results.The samples were negative for TF when using real-time PCR and the few positive all had cycle threshold (Ct) values above 35.

Celiac disease (CD) is an autoimmune disease, induced by an immune mediated reaction in the small intestine after ingestion of gluten and related prolamines. There is a strong genetic linkage and most important are the genes that encodes for HLA-DQ2 and HLA-DQ8. The European Society for Paediatric Gastroenterology, Hepatology and Nutrition (ESPGHAN) has developed new guidelines for investigation of CD in children and adolescents, which include genotyping for HLA-DQ2 and HLA-DQ8. Therefore, the clinical microbiological laboratory in Västmanland wants to introduce a method for analysis of these genes. Microarray is a newly developed method for determination of the alleles HLA-DQA1 and HLA-DQB1 that encodes for the ?- and ?-subunits of the HLA-molecules.

The aim of this study was to investigate the intraoperative surgical stress response in dogs undergoing ovariohysterectomy and to compare acepromazine and medetomidine as premedications. 15 dogs diagnosed with pyometra were used in this study. Blood pressure and heart rate were used as parameters to measure surgical stress response. The surgery was divided into four phases. Phase 0 was the period 10 minutes before the skin incision, phase 1 was skin incision and opening of abdomen, phase 2 was manipulation of uterine horns, ligation and transection of mesovarium and phase 3 was ligation of cervix, removal of organs and closing the abdomen. The results showed that phase 2 was the most intense phase of surgical stress, regardless of which premedication the dogs received. When acepromazine and medetomidine were compared the results showed that within phase 3, all dogs that were given medetomidine had higher blood pressure compared to dogs that were given acepromazine. The dogs that were given acepromazine had higher heart rate than the dogs that were given medetomidine in phase 0, phase 2 and phase 3. .

People live longer. There is a growing need to increase the knowledge of aging and of the need among the elderly for medical care in the near future. As a step towards meeting this need, a survey among the elderly in Skåne is made by the division of Geriatric Medicine in Malmö University Hospital. This survey is called Gott åldrande i Skåne, GÅS. A frequent decease among the elderly is Mild Cognitive Impairment (MCI).

Clinical signs of hypocalcaemia in dairy cows are easily recognized, and a rapid response to treatment generally confirms the diagnosis in the field. However, clinical signs of other disorders in cows, such as toxemia, calving paralysis, or muscoskeletal injury, may mimic those associated with hypocalcaemia. Therefore, making a diagnosis of hypocalcaemia on the basis of clinical signs alone may be difficult. In ambulatory veterinary practices, cow-side tests are useful to make the diagnosis and prognosis easier. Kruuse?s Kalcium Test is a cow-side test based on the principle that calcium ions are necessary for coagulation of blood, and that chelation with EDTA prevent coagulation.

Celiac disease (CD) is an autoimmune disease, induced by an immune mediated reaction in the small intestine after ingestion of gluten and related prolamines. There is a strong genetic linkage and most important are the genes that encodes for HLA-DQ2 and HLA-DQ8. The European Society for Paediatric Gastroenterology, Hepatology and Nutrition (ESPGHAN) has developed new guidelines for investigation of CD in children and adolescents, which include genotyping for HLA-DQ2 and HLA-DQ8. Therefore, the clinical microbiological laboratory in Västmanland wants to introduce a method for analysis of these genes. Microarray is a newly developed method for determination of the alleles HLA-DQA1 and HLA-DQB1 that encodes for the ?- and ?-subunits of the HLA-molecules.

Fossil fuels are affecting our climate negatively and there is a limited amount of them in the world, which leads to the importance of finding alternative fuels. One alternative is biogas, which is produced though a digestion process of different organic materials in a biogasreactor. Organic substrate that comes from farms has the biggest potential to increase the production of biogas in Sweden. Primarily crops from farms, but also waste products like manure are of great importance.Today there is a calculated theoretical potential for biogasproduction of 14TWh per year, which is around ten times larger than Sweden?s production today.

Platelets are important for the healing of damaged blood vessels since they have an importantpart to play in the coagulation process. At the same time, the blood must be kept fluid and notcoagulate at the wrong time. Therefore there are factors that effect the aggregation of plateletsin a positive or a negative way.Previous investigations have shown that platelets during stirring conditions produce reactiveoxygen species (ROS) that weaken the inhibiting effect of nitric oxides (NO) on platelets andthat the drug Dexamethasone (Dex) can reduce the ROS-production.The aim of this project was to investigate if glucocorticoids, in this case Dexamethasone,could restore the inhibiting effect of NO on platelets and if there was any decrease in ROS-production.The result of the ROS-measurements showed a great variance and it was difficult to draw anyconclusions from them, but a clear decrease in ROS, as previous reported, was not shown. In the aggregation experiments the inhibiting effect of NO was observed through the drug S-nitroso-N-acetylpenicillamine (SNAP), a NO-donator.From the aggregation experiments, the result seemed to be that SNAP during longerincubation time lost its inhibiting effect, probably because the cells become desensitized.With superoxid dismutase (SOD), the effect of SNAP increased, both in the experiment withlonger and shorter incubation times. Dex seemed to reinforce the aggregation in relation toboth SOD and SNAP.

The purpose of this investigation was to find out more about students? spontaneous ideas about properties and transformations of matter. Examples of matter chosen are such which students know about from everyday life and focus is mainly on the questions if students know that all matter has weight and that matter is preserved in transformations.The method used is semi structured qualitative interviews with individual students. Different experiments were carried out together with the students who afterwards were interviewed. However, the students show varying answers and no generalizations can be done.

Staphylococcus aureus is the most clinically important Staphylococcus species and is associated with high mortality in patients with positive blood cultures. S. aureus bacteria may cause a variety of disease manifestations ranging from minor skin infections to life-threatening conditions such as pneumonia, meningitis, osteomyelitis, endocarditis, toxic shock syndrome (TSS) and sepsis. This microorganism belonging to the gram positive cocci may also be part of the normal flora. In Sweden, penicillinase-stable penicillins are the primary alternatives to treat S.

It is important for today?s farmers to minimize the costs for crop establishment, because of decreased prices and support from the EU. Farmers, who plow in autumn on heavy clay soil, often do some kind of tillage in autumn to get better soil structure before spring seeding. This is especially important when growing small seed crop, e.g. sugar beet and oil plants.

Inledning: Sjukvården är beroende av frivilliga bloddonatorer. Det behövs alltid fler bloddonatorer då vi med dagens sjukvård kan utföra alltmer avancerad kirurgi samt rädda och hjälpa allt fler sjuka samt skadade, med hjälp av blod från frivilliga donatorer.Syfte: Syftet var att undersöka vilka skäl som anges till att inte donera blod, samt undersöka om viljan att ge blod skiljer sig beroende på kön, ålder och om man har yrkes- eller studieanknytning till sjukvård. Syftet var också att undersöka vilka faktorer potentiella blodgivare anger som motiverande till att donera blod.Metod: Denna uppsats baseras på en kvantitativ tvärsnittsstudie, bestående av en webbenkät som delats av författarnas på det sociala mediet Facebook. Enkäten vände sig till personer som enligt blodcentralens kriterier får donera blod, totalt inkom 90 svar, med ett bortfall på totalt 11 personer. Enkäter analyserades sedan kvantitativt med hjälp av statistikprogrammet SPSS.Resultat: Studien visar att den vanligaste orsaken till att respondenten inte gått och donerat blod är att de tillfrågade har tänkt på det men att det inte blivit av.

Pyometra, chronic purulent metritis in metoestrus, is a common disease in intact bitches. About 25% of all bitches in Sweden have contracted the disease before ten years of age. Clinical signs of the disease are varied, and the course may be either slow or acute. The diagnosis is most commonly made by radiographical or ultrasonographical examination of the abdomen in combination with blood biochemical and hematological analyses. The safest and most efficient treatment of pyometra is surgical ovariohysterectomy.There is room for improvement of both diagnostic tools and possible prognostic indicators for pyometra.Mast cells are large leucocytes, with granule that contain inflammatory mediators.

The purpose with this study was to investigate how the behaviour and physiological parameters differ between high ranked and low ranked cows, and between cows in an automatic milking system verses conventional milking parlour. The study was carried out at the Kungsängen research Centre, Department of animal nutrition an management, Swedish University of Agriculture Sciences, Uppsala. Twelve cows, the six highest and the six lowest in social rank were selected from the automatic milking system for the experiment. Ten cows, the five highest and five lowest in social rank were selected from the conventional system. Blood samples were taken during milking and resting.

Staphylococcus aureus is the most clinically important Staphylococcus species and is associated with high mortality in patients with positive blood cultures. S. aureus bacteria may cause a variety of disease manifestations ranging from minor skin infections to life-threatening conditions such as pneumonia, meningitis, osteomyelitis, endocarditis, toxic shock syndrome (TSS) and sepsis. This microorganism belonging to the gram positive cocci may also be part of the normal flora. In Sweden, penicillinase-stable penicillins are the primary alternatives to treat S.