In situ sekvensering är en metod som kan användas för att lokalisera differentiellt uttryck av mRNA direkt i vävnadssnitt, vilket kan ge viktiga ledtrådar om många sjukdomstillstånd. Idag förloras många av sekvenserna från in situ sekvensering på grund av det kvalitetsmått man använder för att säkerställa att sekvenser är korrekta. Det finns troligtvis möjlighet att förbättra prestandan av den nuvarande base calling-metoden eftersom att metoden är i ett tidigt utvecklingsskede. Vi har genomfört explorativ dataanalys för att undersöka förekomst av systematiska fel och korrigerat för dessa med hjälp av statistiska metoder. Vi har framförallt undersökt tre metoder för att korrigera för systematiska fel:I) Korrektion av överblödning som sker på grund avöverlappande emissionsspektra mellan fluorescenta prober.II) En sannolikhetsbaserad tolkningav intensitetsdata som resulterar i ett nytt kvalitetsmått och en alternativ klassificerare baseradpå övervakad inlärning.III) En utredning om förekomst av cykelberoende effekter, exempelvisofullständig dehybridisering av fluorescenta prober.Vi föreslår att man gör följande saker:Implementerar och utvärderar det sannolikhetsbaserade kvalitetsmåttetUtvecklar och implementerar den föreslagna klassificerarenGenomför ytterligare experiment för att påvisa eller bestrida förekomst av ofullständigdehybridisering.

The power system modelled in this thesis work has been developed for simulationwhen e.g. the production in some part of the power system is changed. The powersystem has been modelled according to the swing mass of the system, the frequencydependency of the load and the turbine governor. The model determines the systemfrequency, the period of regulation and the power exchange between the differentsubsystems in the Nordic synchronized system.In this model the system frequency is assumed to be the same in all different parts ofthe power system and the voltage conditions in the power system are neglected. Forthat reason power swings does not occur between different subsystems in the powersystem, which usually occur as a result of the swinging connection between thetransmissions line.

The intention of this essay has been to investigate how the new digital technology has affected the tourism industry and marketing, as well as how travel agents and tour operators adapt to this. We also aimed to get a broader view of how the future might look like when it comes to marketing and virtual service landscapes. We decided to base our research according to these following four questions:? What affect travel agents? way of adoption to the technical development?? What purposes do the travel agents have for building up their virtual service landscape?? How much does the travel agents emphasize on digital marketing?? What can travel agents do to better adapt to the technological development in the future?We have chosen to use a qualitative research for this essay. The reason for choosing a qualitative research was to gain a wider understanding that would represent a big part of the changes of technology development.

This study has its background in the debate about religion and the existence of God, which has been an ongoing issue throughout the Western cultural tradition. Today´s information society has been an impact on the increasing interest for this subject. The essay?s main task is to accomplish a rhetorical analysis of two books, Illusionen om Gud (2008) by atheist Richard Dawkins and Guds dödgrävare (2010) by Christian John Lennox, in order to investigate the authors? use of rhetorical strategies to influence their audience. The texts are studied using a qualitative approach with the theoretical basis of some well-defined rhetorical variables: ethical, logical and pathetic means of persuasion, propaganda, and the important factor that a message always is presented in a certain context in which the recipients both have their own values and subjects to general truths and common frames of reference.

This master thesis has been a product realization process. Having BRIO:s walking cart as thestaring point the purpose was to further develop the principle of such products and at the sametime broaden their sector of use. The final result is to be regarded as a complete solutionmeant as a foundation for further development at the BRIO Company. The product aims tosatisfy the demands and needs of the target group called theon the go parent. Trends, qualityand purchase power are features that characterize this particular target group.The project resulted in a walking stool namedBRIO Circus after its source of inspiration.

An ever-increasing population, urbanization and visions towards sustainable cities clarifies the need of densification. When densifying existing urban areas adding storeys could serve as a residential densification, which would also meet the market demand for centrally located apartments. An increased understanding of the potential of vertical densification gives way to the aim of this thesis to increase the amount of centrally located housing.This thesis provides knowledge of adding storeys and how property owners can identify opportunities in their existing building stock.The first question, What kind of residential densification takes place in Sweden today? highlights ways of residential densification; development of brownfields, infill and adding storeys, where adding storeys is what this thesis then focuses on. The result came through a literature review that also clarified important factors when adding storeys.

During the recent years the interest in entrepreneurial education has increased both in terms of research being made in the subject, but also in the extent of entrepreneurial courses in Swedish secondary schools, where as the concept Junior Achievement (JA) is particularly being used. A common goal of the entrepreneurial education is apart from teaching the students how to start and run a business, to develop the students? entrepreneurial skills and competences. The main purpose of the essay is to describe and illustrate the role of the teacher in the entrepreneurial pedagogy being used in courses with the JA concept and to investigate which entrepreneurial skills are being developed by the students participating in these courses. There is also the purpose of investigate what the characteristics are of this entrepreneurial pedagogy being used in the courses using the JA concept.

Angelica archangelica (Garden angelica) is the only Medicinal and Aromatic Plant (MAP) with a Nordic origin. The plant can reach up to three meters when cultivated. Angelica archangelica is used as flavouring in additives, honey, beverage base, essential oils, fol-klore medicine and as ornamental for decorative purposes. Commercial cultivation is mainly focused on root production. Production countries are Poland, Netherland, France, Belgium, Switzerland, and former Czechoslovakia with an overall yearly world production of 1000 kg of essential oils.

This study is a research on how dystopian features are expressed within different genres. The purpose is to discuss films that contain dystopian features in relation to genre and to examine if there are shared conventions in the films that can make dystopia a film genre on its own. The theoretical base includes genre theory and Rick Altman?s semantic/syntactic approach to film genre. Five films from different genres, all produced within the time period of 2000-2010, are analyzed with a semantic/syntactic approach to genre and then discussed in relation to dystopia and prior research.

The purpose of this thesis has been to; based on interviews with four teachers in Stockholm city, get at picture of preschool teacher?s viewpoint on children?s influence in preschool.Research questions: How is the children`s influence looked upon by the teachers in preschool?, In what ways do children?s influence affect their learning, In what ways are the interviewed preschool teachers working with democracy in preschool?To carry out this thesis, I used the qualitative method. In the qualitative method, use is made of the interview method to collect empirical data with interviews. I interviewed four preschool teachers from three different preschools. This thesis is built upon John Dewey?s philosophical thinking as a theoretical base.The result of this thesis shows that all preschool teachers felt that the application of children?s influence in the daily activities, although complicated, is essential in their work.

Climate and environmental issues is now high on the agenda. We live in a generation that must try to solve some major environmental problems. Buildings and habitations account for approximately 40 % of Sweden's total energy today. To reduce this figure, as needed, more research in the field of energy efficiency is required.This thesis concerns the heating of buildings. The load on district heating is heavy during certain times of the day.

Debate over Intellectual Property Rights ?IPRs? particularly patent and copyrights is mainly on forward-looking industries in computer software. As part of a trade deal reached in 1994, the member nations of the World Trade Organisation must adhere to a global agreement known as TRIPS, for the Trade- Related Aspect of Intellectual Property Rights.This study is to analyse the ethical conception of Intellectual Property Rights and in particular its implications on the developing countries in relation to TRIPS. The approach will be to analyse a broad philosophical theories of property to see if there is any justification for a software program to be treated as private property and also argue base on John Rawls two principles of justice in relation to TRIPS Agreement. Some reflections will be put on the use of open-source software by less developing countries.From the study it was asserted that, strong IPRs protection would hinder technological transfer and indigenous learning activities in the early stage of industrialisation when learning takes place through reverse engineering.

This is the project of making a stage design for Batboy ? the musical, a cooporation with a network of musical artists. The aim of the project was to create a space on stage that strengthens and expands the experience of the story told, and find design solutions that give an extra dimension to the story. The prerequisites for my work were partly defined from the produc¬tion, as were my limitations. In the cast of ten artists some were to play more than one role, often one of the opposite sex.

This paper is based on a study of the Christian religious school. In Sweden today, surveys have shown that there is a common fear and negative attitude towards Christian religious schools. Despite this fact these schools increase in numbers, which makes it relevant to investigate what reasons the parents base their decision on when making Christian religious schools their school of choice for their children. The formulation of the question in this paper is therefore based on why the parents choose to place their children in a Christian religious school. Previous research on this topic is very scarce and poor, which makes it a relevant topic to investigate more thoroughly.

The aim with this study is to investigate the market reactions to announcements of special dividends and redemptions in Sweden and thus if these announcements can signal information. This study is an event study, where the event is the day of the announcement of a suggestion regarding issuance of special dividends or redemptions. The abnormal returns were estimated for two samples with the market adjusted returns model, one including special dividend announcing firms and the other redemption announcing firms. The signalling hypothesis and the hypothesis of a tax induced clientele effect are the most important hypotheses for this study. The efficient market hypothesis is another theoretical base that may explain the market reactions to the studied announcements, especially the pre-announcement activities that may occur.