Blood samples from 44 clinically healthy cats were analysed for the presence of Antibodies to Chlamydophila felis. As we wanted to investigate if the antibody titre of clinically healthy cats correlates to shedding of chlamydia, conjunctival and vaginal or preputial swabs respectively, were taken and analysed with rtPCR (real-time polymerase chain reaction) for the presence of chlamydia DNA. Furthermore, to investigate possible epidemiological factors connected with asymptomatic cats infected with chlamydia and to see if there were any indications that chlamydiosis can cause reproductive problems, the owners of the cats were asked questions regarding this. Sixty-one per cent (27 of 44) of the cats were seropositive. Three of these (11 %) had the highest antibody titre (1:1600).

ABSTRACT Tobacco Rattle Virus (TRV) is a soilborne plant virus. It is an important pathogen in potatoes where it causes spraing disease. This disease can lead to significant economical loss for the farmer, so it is imperative to get the proper diagnose before planting. TRV is spread naturally by free-living nematodes (Trichodorus and Paratrichodorus). Transference of TRV to a plant occurs when the virus-carrying nematodes feed on plant roots. The relationship between virus, nematodes and plants is complicated, and has to these days not been fully characterized. The diagnostic methods available today do not work sufficiently.

Milk allergens were digested by allowing them to flow through a chromatography column, where pepsin was conjugated to the stationary phase of the column. The allergen fragments were then characterized both biochemically, by using SDS-PAGE and gel permeation chromatography, and immunologically, by examining their reactivity to IgE and monoclonal Antibodies..

Målet med detta examensarbete är att minska risken för olyckor där grävmaskiner, med borr- eller pålmast monterad, är inblandade. För att kunna uppnå detta mål, har ett beräkningsprogram skapats som definierar ett säkert arbetsområde innan ostabilitet inträffar. Detta har gjorts genom att studera tidigare forskningsarbeten inom området samt verifiering av beräkningsprogrammet med hjälp av en fallstudie och data om maximal lyftkapacitet för grävmaskinen..

Background. Immunohistochemistry is a useful method in the differential diagnosis between pleural mesotheliomas and metastatic adenocarcinomas in the pleura. Cytokeratin 20 and 7 have been used successfully as markers in studies determining primary location of adenocarcinomas from metastases. The current study is a complementary research of archived paraffininbedded material of cases with cancer origin. This study contributes a bigger statistical material that may facilitate the search for unknown primary site of adenocarcinoma by identification of metastatic cells in the pleura.Methods.

Immunmedierad trombocytopeni (IMT) är en vanlig rubbning i primära hemostasen och uppstår som en konsekvens av för tidig destruktion av trombocyter (blodplättar). Denna destruktion beror på en ökad produktion av trombocytbundna antikroppar (platelet-bound Antibodies, PBA) som binder in till antigen i trombocytväggen. Antikroppsproduktionen, som är en immunologisk process, kan dels uppstå spontant genom en autoimmun reaktion (primär IMT), eller triggas av infektioner eller neoplasier (sekundär IMT). Diagnosticering av IMT, och då syftas framför allt på primär IMT, sker främst utifrån uteslutande av andra bakomliggande orsaker, då en specifik diagnostisk metod inte finns tillgänglig. Tester för att påvisa PBA finns, men deras betydelse för både diagnostik och prognos har länge varit oklar. Denna uppsats syftar till att sammanfatta forskning som finns tillgänglig om betydelsen av påvisande av PBA vid IMT, men även forskning kring den immunologiska bakgrunden till sjukdomen.

Japanese encephalitis virus (JEV) is one of the leading causes of acute encephalitis in humans. The virus is spread by mosquitoes, mainly belonging to the Culex species. The main reservoirs are considered to be birds and pigs, with pigs constituting the most important reservoir in regard to human infection. During the last few decades the production of pork has increased significantly in Vietnam. With 90% of pigs being kept in households owning 10 pigs or less, pigs are widely spread across most parts of the country.

Spitz nevus was first described by Sophie Spitz in 1948 as juvenile melanoma. The lesion is a benign melanocytic tumor, which consists of epiteloid- and spindelshaped cells. Histological is spitz nevus difficult to distinguish from malign melanomas and spitzoid melanomas. Loss of symmetry, loss of maturation in the deep component, nuclear polymorphism and hyper chromatic nucleus are features which can be found in melanomas. Some of these features are often seen in spitz nevus.

The world?s population reached more than 6.9 billion people in July 2011. It is expected that the population growth during the next 40 years will take place mostly in low-income countries and the population increase will be 2.3 billion people during 2011-2050. A rapid urbanization is also expected to result in 57.7 per cent of Africa?s population living in urban areas in 2050.

The aim of this study was to determine the prevalence of Antibodies against the intracellular protozoan Encephalitozoon cuniculi in healthy pet rabbits. Encephalitozoonosis is an important differential diagnosis to ascending infection with Pasteurella multocida in the ears since a common symptom is vestibular syndrome. Samples were collected from healthy rabbits that were brought to an animal clinic in Stockholm for some kind of prophylactic procedure. The samples were analysed by an India-ink Immunoreaction test / Carbon Immunoassay. Of 106 rabbits 7 (6.6%) were seropositve.

Reliable methods to analyze food for the presence of almond are important ? not only for those allergic to almond, but also for monitoring the compliance with labelling regulations (EG directive 2003/89). Until now the Swedish National Food Administration has used methods like rocket immunoelectrophoresis and real-time PCR to detect almond in food. These methods are, however, not sensitive enough for protecting the most sensitive individuals. Therefore, the performance of a commercial ELISA kit was tested with regard to specificity/cross reactivity and limit of detection for almond both in solution and in different matrixes.The limit of quantitation was at least 3,1 ppm (mg/kg) in solution and similar concentrations were measured in bisquits and chocolate.

Porcine circovirus type 2 (PCV2) is a common virus present in most parts of the world. PCV2 has been pointed out as the major causative agent to post weaning multisystemic wasting syndrome (PMWS) that affects pigs after weaning. The clinical symptoms of PMWS are impaired growth, diarrhoea, respiratory problems and increased mortality. At autopsy enlarged lymph nodes with depletion of lymphocytes are commonly found. Since 1991 PMWS has spread all over the world and was first described among Swedish pigs in 2003. Although PCV2 is widespread, only some pigs develop PCV2-related diseases. Why this is and what other factors that are required in order for the disease to develop is yet unknown. The main theories involve other infections, for example PRRS, and management issues but also genetic differences between pigs and/or virus strains have been suggested.

The United Nations has, during many years, developed a model for a globally harmonized system for classification and labeling of chemicals, with the aim of it becoming a global standard. This system is implemented in the European Union through the CLP-regulation and is now working parallel to the existing directives until 1st of June 2015, when all the new classifications of substances and mixtures must be completed. The aim of this project was to adjust the laboratory to the new legislation, through inventory of stored chemicals, update the list of chemicals and perform self-classifications of mixtures. The work included handling of about 650 different chemicals with varying hazard classifications and search for information in corporate websites and chemical databases. This resulted in a new and complete list of chemicals stored and used in the laboratory and, in addition, an example of how to make a self classification of a mixture.

Det finns många olika typer av bromerade flamskyddsmedel. De är alla stabila i miljön men graden av toxicitet och bioackumulering varierar. Att bromerade flamskyddsmedel är endokrinstörande är känt men min hypotes är att de även är neurotoxiska och att de kan orsaka neurologiska störningar hos människa. Människor exponeras för bromerade flamskyddsmedel via damm och föda. Barn och foster som är känsligare än vuxna kan även exponeras via modersmjölk respektive placenta. Barn har högre serumkoncentrationer av bromerade flamskyddsmedel än vuxna.

Many farms in the counties in the south of Sweden have difficulties with diseases spread by ticks; mainly babesiosis and tick-borne fever (TBF) but there are indications that borreliosis ought to be included. All of the tick-borne diseases radically decrease farm profit if the herd is affected. The production of the vaccine used against babesiosis since the 1920´s, will be discontinued in the near future and in addition to economical reasons it is therefore important to learn more about the diseases above. During the summer and fall of 2002 and 2003, a farm in the island of Gotland was burdened with unusually many cases of bovine babesiosis. In total 43 animals fell ill and one died.