This report describes a thesis done at the Jönköping University of Technology. The aim of this thesis was to create conditions to optimize an existing automatic electrostatic coating system by changing the technology for dispensing lacquer. To select the technology, a study of different dispensing techniques carried out which concluded that the piezoelectric membrane and electrostatic atomization are the most interesting techniques.In order to perform tests with dispensing techniques a demonstrator has been optimized. The controller and PC software of the demonstrator has been programmed to be able to send out PWM signals that control the dispensers. An adapter card was designed to control the piezoelectric membrane and a steering link to the electrostatic atomization has been developed for PWM signals up to 500V.The work has laid ground for a demonstrator where further testing of the dispensing technologies can be performed..

To achieve the Government's milestones for 2018, which means that 50 % of food waste shall be separated from household waste and undergo biological treatment (composting or anaerobic digestion).   Hultsfred, Högsby and Vimmerby are three adjacent municipalities of Kalmar, county Småland. During 2012 and 2013 analyzes were performed on their household waste to determine its composition. The household waste is currently collected in one fraction. The work, examines how much food waste the household waste contains. From the amount of food waste, the biogas potential is examined - how much food waste biogas generates and how much energy it corresponds to.   Food waste is broken down by microorganisms under anaerobic conditions (anaerobic digestion) in a biogas plant.

In the coming years, organic food waste will be collected in the region of Gästrikland, Sweden. The collection is planned to cover the entire region by the end of year 2007. To start with, smaller amounts are being collected and transported to a central composting plant in Sala. However, a central composting plant in the region of Gästrikland is projected.The objective of this thesis is to evaluate a number of possible methods for composting of organic food wastes regarding their environmental impacts and localization.There are four scenarios for future composting of organic waste that have been evaluated. There are two that consist of membrane composting, either in Sala or in the region of Gästrikland.

Oxytocin is a hormone which plays a crucial role in many reproductive and behavioural functions. It affects many organs and the classical peripheral targets are the mammary glands during lactation and uterus during labour. Oxytocin receptors have recently been described in a variety of normal tissues and primary cell cultures, but also in neoplastic tissues and established neoplastic cell lines, as breast cancer cell lines. The signalling pathways and biological effects of the oxytocin/oxytocin receptor system seem to depend on species, type of tissue, physiological versus neoplastic state and receptor location within the cell membrane.The aim of this project was to find out if the cell proliferation in the canine mammary carcinoma cell line CMT-U27 was affected upon stimulation of oxytocin, and to investigate the presence of oxytocin receptors in these cells. The cell proliferation was examined by using an ELISA-kit, where the absorbance measured is relative to the amount of living cells.

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Today's society is facing major challenges. In order to reduce the climate impact fossil fuels should be replaced with fuels that do not contribute to the greenhouse effect. The growing population generates organic waste originating from industry and households so called organic fraction of municipal solid waste (OFMSW). Through anaerobic digestion, waste can be utilized to produce energy-rich methane gas. In this way, waste can be a resource instead of a burden on society.

Wastewater handling of private sewage systems has become an all increasing matter. In Stockholm region the 90 000 private sewage systems contribute with larger phosphorus flows to the Baltic Sea than the two biggest sewage plants in the area, Henriksdal and Käppala, contribute together, even though these two plants purify wastewater from more than one million inhabitants. When summer house areas turn into areas for permanent living ? transition areas ? the load on the existing systems increases, systems that already often are unsatisfying. In those areas it is not clear what the best system choice is; On-site systems for single households; Local collective systems or Connection to central systems.

Biogas is a renewable energy produced through anaerobic digestion, which means that organic matter is degraded by microorganisms under anaerobic conditions. The produced biogas can then be used for cogeneration, electricity, heat or upgraded to vehicle gas. Eskilstuna Energy & Environment AB has four digesters at Ekeby water sewage treatment plant, Eskilstuna. One of the biogas reactor (RK4) is equipped with a jet-mixing system while the remaining digester uses top-mixing. The mixing system is an important parameter to achieve optimum operating conditions for the process regarding gas production and degree of digestion.

This master thesis is a study of anaerobic thread lockers and is done in cooperation withÖhlins Racing AB, which is a Swedish manufacturer of vehicle suspensions.The suspension in vehicles is constantly exposed to high vibration levels and a suspensionunit has several screw- and cylindrical joints. As an extra safety precaution so that thecomponents in the suspension doesn?t fall apart when using the vehicle, an anaerobicthreadlocker is added in these joints. The handling of these threadlockers is not totallyharmless and could lead to allergic reactions, and therefore there are Swedish laws to limit theuse of these. However, new types of anaerobic threadlockers have recently been launched onthe marked that are not considered under this law.

Results have shown that flow batteries may be a solution in the future as an effective and environmental friendly method to an energy storage system (ESS). The technology is reliable and has a high efficiency that comes with low energy losses and a long lifetime. The range of possible fields is suitable for cutting energy peaks in the power grid, by always have a ready and available energy storage that balances the production. By comparing the advantages of flow batteries with conventional batteries it is mainly the fact that they can conserve energy for a long time without being self-discharged thanks to that the storage capacity is in principle endless and limited by the size of the electrolytes tanks that makes them a great energy storage system. The batteries won?t take any damage or decrease in performance when charging or discharging it or if you exhausts it to 100 % and leave it discharged for a long time.

The goal of this master thesis project was to develop computer models of some plausiblepretreatment techniques and to assess the potential benefits/costs of using pretreatment andanaerobic digestion for waste management of organic waste from a system perspective. Forthis purpose a computer program called ORWARE has been used. ORWARE (organic wasteresearch) is a program for making life cycle assessments of waste management. As the mainpart of the project work the ORWARE system has been provided with three new models ofpretreatment techniques. The additions consist of: One model of pretreatment with ultrasound,one model of thermal hydrolysis and one model of a screw press.

The aim of this study was to establish knowledge about the most suitable substrate for cultivating different kinds of anaerobic beer spoilage bacteria in the brewing industry. During the study, two other aspects were investigated; if the sample volume affects the results and which anaerobic beer spoilage bacteria are present in the micro flora at the brewery. The method used was standard cultivation with four different substrates; Universal Beer Agar, Nachweismedium Bierschädliche Bakterien, Raka Ray and VLB S7-S. During the identification of bacteria in the micro flora, a method using PCR was used. None of the substrates showed a significant advantage over the others.

Käppala wastewater treatment plant situated on the island of Lidingö northeast of Stockholm is running a project during 2004 and 2005 with the purpose to map out the capacity of anaerobic digestion in the digesters that treat primary and excess sludge. The purpose of this thesis work, which is part of that project, was to characterize the present anaerobic digestion process and to investigate its capacity to treat other organic wastes such as restaurant waste and waste from water works. To decide the potential of both methane and biogas production from different substrates batch laboratory tests were carried out. To imitate the anaerobic digestion process at Käppala continuous tests with small scale reactors were carried out. These reactors were later fed with restaurant waste.The batch laboratory tests showed that primary sludge had a potential biogas and methane production of 0,62 and 0,35 Ndm3/g VS respectively after 40 days of digestion.

Biogas is a renewable energy source that is produced by anaerobic digestion of organic mate-rial. In Sweden, biogas predominately comes from sewage water sludge and landfills or from organic waste of households and industries. Small scale digestion plants at farms are espe-cially expected to contribute to increased biogas production in the future. Biogas can be ob-tained directly in it?s raw form and used as fuel in a combustion chamber.

The Annexin-V/propidium iodide (AV/PI) assay detects early membrane changes in spermatozoa and viability using flow cytometry. It is an objective method that evaluates a much larger number of single spermatozoa compared with the usual estimation of motility, the most commonly used method for routine sperm evaluation. In the present study four ejaculates from four stallions were used in a split-sample design to investigate whether and how the number of intact spermatozoa is affected over time and after incubation at different temperatures, either those recorded during shipment of stallion semen for AI (9 or 5°C) or room temperature (20°C). The Annexin-V results did not reflect the motility results that were monitored in parallel. While motility decreased over time, the number of spermatozoa with intact membranes (viable) remained unchanged for at least 21 hours following a short period of initial instability.