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… and human medicine a radioactive based test (Thymidine kinase radio-enzymatic assay: TK-REA) is commercially available to assess TK1 serum activity. For example, it is used to determine TK1 serum activity in dogs. However, TK-REA requires handling of radioactive material and …
… in foods. The optimization involves modifications of an already existing assay. Three synthetic DNA templates, representing the different genogroups of human sapovirus, were amplified by real-time one-step RT-PCR. The modification of the RT-PCR assay involved testing TINA …
… the IncuCyteTM FLR image system and fluorometric microculture cytotoxicity assay. Using the IncuCyte FLR method allows for detecting changes in the molecular processes of living cells. To make proteasome inhibition visible the model cell line MelJuSoUbG76V-YFP is used which …
… studied which will contribute to different technical issues when performing an assay. The aim with the project is to optimize and develop the high throughput protein detection technique Proximity Extension Assay, PEA, into a 96-plex panel, in hopes of discovering an expression …
… controlprogram as well as by a commercial prototype of a multiplex immunoassay manufactured byLuminex Corp., which is currently under evaluation at the United States Department ofAgriculture USDA. This 4-plex assay detects antibodies for the three above-mentionedviruses as well …
… are updated regularly. In 2014, Cepheid released their Xpert Flu/RSV XC assay, which can detect Influenza A and B and RSV by multiplex RT-PCR in approximately one hour. The aim of this study was to evaluate this assay at Laboratoriemedicin Västernorrland by using the …
bioassays with the insect herbivores Spodoptera littoralis and Agriotes spp.
… of wild and domesticated cotton in three different experiments. A feeding assay was conducted, the mortality, development time and pupal weight of larvae of the Egyptian cotton leaf worm Spodoptera littoralis was studied. In a second experiment the preference of the root-feeding …
… has low capacity to grow in normally used cell cultures. Many specific strand assay detection studies have been done in order to understand more about HEV replication. Unfortunately, these assays have the disadvantage of giving false positive results. Aim: The aim of this …
The Annexin-V/propidium iodide (AV/PI) assay detects early membrane changes in spermatozoa and viability using flow cytometry. It is an objective method that evaluates a much larger number of single spermatozoa compared with the usual estimation of motility, the most commonly …
… antibiotikabehandling. I studiens början sattes en enzyme-linked immunosorbent assay (ELISA) för detektion av antikroppar mot Moraxella catarrhalis upp. I studien undersöktes om antikroppstitrar i serum mot bakterien Moraxella catarrhalis var lägre hos patientgrupperna än hos …
Optimisation and evaluation of 3 assays
… methods for quantifying TPPII. Western blot, enzyme-linked immunosorbent assay (ELISA) and fluorophore-linked immunosorbent assay (FLISA) protocols were optimised regarding incubation times and antibody dilutions. Sensitivity and linearity were the most important parameters when …
… controlprogram as well as by a commercial prototype of a multiplex immunoassay manufactured byLuminex Corp., which is currently under evaluation at the United States Department ofAgriculture USDA. This 4-plex assay detects antibodies for the three above-mentionedviruses as well …
Faktorer som påverkar
… controlprogram as well as by a commercial prototype of a multiplex immunoassay manufactured byLuminex Corp., which is currently under evaluation at the United States Department ofAgriculture USDA. This 4-plex assay detects antibodies for the three above-mentionedviruses as well …
… cardiac disease, and due to a high inter-species sequence homology; human cTnI assays can often be used in veterinary medicine. The main aim of this study was to perform an initial evaluation of a human cTnI test, the Meritas Troponin I test, for measurement of equine plasma …
med avseende på membranintegriteten och membranstabiliteten
… 0h, 24h and 48h. Four groups of spermatozoa were obtained by the Annexin-V/PI-assay: intact, living spermatozoa (AN-/ PI-), spermatozoa with unstable membrane (AN+/ PI-), spermatozoa with damaged membrane but not entirely necrotic (AN+/ PI+) and dead spermatozoa (AN-/ PI+) …
