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… rapid and sensitive methods. In this study, Taq Man RT-qPCR was compared with SYBR Green RT-qPCR (LightCycler480, Roche) to explore which method had the best sensitivity with the least working hours. In addition, Bullet for automated DNA extraction and CAS 1200 ? for automated …
ABSTRACT A defect implantation process is the major reason for unexplained infertility. Estrogen andprogesterone are steroid hormones preparing the endometrium for implantation. They mediatetheir effect through their receptors: estrogen receptor alpha and beta and …
Two species of bees in the genus Apis, real honey bees, has long been of interest for man. These two are the European honey bee, Apis mellifera, and the Asian honey bee, Apis cerana. In Vietnam, beekeeping is of great importance, both with A.cerana and A.mellifera. The aim of …
… bland de bästa utifrån kraven. Dessa var ATP-analys, LIVE/DEAD® BacLight? samt SYBR Green I. Från artiklar hittades fyra nyligen publicerade system som var av intresse utifrån kraven. Dessa var BioMEMS (eng. Biomedical Micro-Electro-Mechanical Sensor), odling i slutna …
… DNA från MT-4 och MO-T cellinjer. Under optimering av realtids-PCR användes SYBR Green och smältpunktsanalys där flera komponenter bl.a. templat-, primer-, magnesium- koncentrationer samt annealing/elongeringstemperaturen modifierades. Efter avslutad optimering utfördes …
