Koncentrisk hämning och stimulans av bakterietillväxt i agarkulturer
AllelopathyConcentric growthInhibition zonesPeriodic precipitationAllelopathy bacterialAgar diffusionBacterial growthLiesegang ringsSarcina luteaBacillus subtilisEscherichia coli (E. coli)NatriumdodecylsulfatAmoxicillin
The aim of this study is to elucidate factors that effect growth of Sarcina lutea and Bacillus subtilis, exposed to the growth inhibitor SDS (Sodiumdodecylsulfat). Agar diffusion experiments revealed repeated, concentric zones of inhibition and stimulation upon exposure to Sodiumdodecylsulphate or to Amoxicillin. Temperature, nutrient concentration and inhibitor concentration were controlled. Formation of successively repeated zones of inhibition, stimulation, inhibition and stimulation is discussed: ? The extension of the primary inhibition zone is due to the concentration of applied Sodium dodecyl sulphate.? Immediately outside the primary inhibition zone the bacteria have access to diffusing nutrients that have not been consumed in the primary inhabitation zone.? In zones of dense bacterial growth the bacteria may produce inhibiting substances, affecting growth of bacteria in adjacent zones.? In zones of dense bacterial growth the nutrients will soon become depleted, thus affecting bacteria in adjacent zones.