The aim of this study was to determine the prevalence of antibodies against the intracellular protozoan Encephalitozoon cuniculi in healthy pet rabbits. Encephalitozoonosis is an important differential diagnosis to ascending infection with Pasteurella multocida in the ears since a common symptom is vestibular syndrome. Samples were collected from healthy rabbits that were brought to an animal clinic in Stockholm for some kind of prophylactic procedure. The samples were analysed by an India-ink Immunoreaction test / Carbon Immunoassay. Of 106 rabbits 7 (6.6%) were seropositve.