Nuts of all kinds are common ingredients in food. For nut allergy sufferers the frequent use of nuts cause problems and "hidden" nuts in food products may elicit allergic reaction when such foods are consumed. Methods for detecting and quantifying walnut (and other nuts) with high sensitivity and Specificity are therefore very important.The objective of this project was to verify the Specificity of a rabbit antiserum against walnut with immunodiffusion and to determine the size of the dominant walnut antigens with Western blotting. In addition, a commercial sandwich ELISA for walnut quantification was validated and compared with a qualitative real-time PCR.The rabbit antiserum proved to be less specific but after absorption with cross-reacting nuts and seeds it showed high Specificity. The ELISA kit reacted, except for walnut, with pecan and slightly with other nuts and seeds tested.

The purpose of this Master's thesis is to determine which of the three general classification schemes, DDC, SAB, and UDC is the most appropriate for classifying music documents. This is done by analysing and classifying 60 music documents such as recordings, scores and books about music. The codes resulting from the classification are analysed according to a number of evaluation criteria based on classification theory. The criteria focus on the systems Specificity, exhaustivity, exclusivity, helpful sequence, hospitality, notation and that related classes are kept together. The study showed that the systems Specificity in classification was of great importance in classifying music documents because of the diversity of music genres.

The aim of this thesis was to evaluate the music downloading service Musikwebb regarding its indexing and retrieval effectiveness. This was done by performing various kinds of search in the system. The outcome of these searches were then analysed according to the criteria Specificity, precision, recall, exclusivity and authority control. The study showed that Musikwebb had several flaws regarding its retrieval effectiveness. The most prominent cases were the criteria exclusivity and Specificity.

The aim of this master thesis is to study the integration in Sweden between forestry and bulky industries as fiber- and sawmilling companies. The analysis is based on theories about transaction cost economies and competitive strategy. The studied companies are active on the Swedish roundwood market. The buying actors on this market were divided into two groups; fiber- and sawmill industries. Three companies from each group were chosen subjectively by the author.

Reliable methods to analyze food for the presence of almond are important ? not only for those allergic to almond, but also for monitoring the compliance with labelling regulations (EG directive 2003/89). Until now the Swedish National Food Administration has used methods like rocket immunoelectrophoresis and real-time PCR to detect almond in food. These methods are, however, not sensitive enough for protecting the most sensitive individuals. Therefore, the performance of a commercial ELISA kit was tested with regard to Specificity/cross reactivity and limit of detection for almond both in solution and in different matrixes.The limit of quantitation was at least 3,1 ppm (mg/kg) in solution and similar concentrations were measured in bisquits and chocolate.

Plant tRNA isopentenyltransferases (tRNA-IPT, EC 2.5.1.8) have two roles, modification of tRNA and contribution to cytokinin synthesis. tRNA-IPT is among the best studied of the tRNA-modifying enzymes, but little biochemical information is available and none for the plant enzymes. Therefore two sets of experiments were conducted. The first one aimed to determine the kinetics and substrate Specificity of the tRNA-IPT AtIPT2 from the higher plant Arabidopsis thaliana. The second project aimed to study the role of IPTs from the moss Physcomitrella patens in the production of cytokinin hormones.

Clinical signs of hypocalcaemia in dairy cows are easily recognized, and a rapid response to treatment generally confirms the diagnosis in the field. However, clinical signs of other disorders in cows, such as toxemia, calving paralysis, or muscoskeletal injury, may mimic those associated with hypocalcaemia. Therefore, making a diagnosis of hypocalcaemia on the basis of clinical signs alone may be difficult. In ambulatory veterinary practices, cow-side tests are useful to make the diagnosis and prognosis easier. Kruuse?s Kalcium Test is a cow-side test based on the principle that calcium ions are necessary for coagulation of blood, and that chelation with EDTA prevent coagulation.

Influenza is a viral infection that affects global health and economy with its endemic and sometimes pandemic spread. Rapid detection of Influenza viruses enables antiviral use and can bring financial savings. It is also essential for the global surveillance of prevalent Influenza strains. RT-PCR is considered the most specific and sensitive method for detection of Influenza, but Influenza mutates at a high rate and it is therefore crucial that RT-PCR methods are updated regularly.In 2014, Cepheid released their Xpert Flu/RSV XC assay, which can detect Influenza A and B and RSV by multiplex RT-PCR in approximately one hour. The aim of this study was to evaluate this assay at Laboratoriemedicin Västernorrland by using the laboratory?s previous PCR assay for detection of Influenza viruses as reference method.Real-time RT-PCR was used to compare Xpert Flu/RSV XC to the reference method.

Background: Hepatitis E (HEV) is a small, non-enveloped virus that belongs to the viral genus Hepevirus. HEV is a positive sense single-stranded RNA virus and there is insufficient information regarding its replication. This is mainly because the virus has low capacity to grow in normally used cell cultures. Many specific strand assay detection studies have been done in order to understand more about HEV replication. Unfortunately, these assays have the disadvantage of giving false positive results.Aim: The aim of this project was to improve the positive strand assay to increase Specificity and eliminate false positivity which is due to high sensitivity of the polymerase chain reaction (PCR).

Authenticity and The Public Space, the paradoxical relationship between them. There focus is put on the need to create a new contextualization for my aesthetic practice, and to make be possible incorporating critical views, questioning both the format and the scenic heritage of the west. ! ! ! I do not believe in authenticity in terms of the more individualistic relations of power but in terms of vulnerability, interdependence and mutual care. Also a way to question how capitalism has shaped and created the way of how we understand ?the? art or the value or the way that it is enjoyed..

Aim Developmental Coordination Disorder Questionnaire 2007 (DCDQ'07) is an international survey tool based on parents' estimation of their child's motor coordination skills. The purpose of this study was to investigate the validity of a Swedish version of DCDQ'07 by finding out how the classification of Developmental Coordination Disorder (DCD) via DCDQ'07 is consistent with the classification of DCD via the motor impairment test, Movement ABC.MethodIn the context of a larger study, a Swedish translated version of the parent survey DCDQ'07 were sent to 4000 randomly selected families with children aged 8 - 10 years in Stockholm County. The classifications "DCD" or "NOT DCD" with cut-off values ??of 56 (9 years) and 57 (10 years) were applied. Forty families were selected from 410 registered responses.

The global biodiversity loss is mainly due to human activities such as an intensification of forestry. Boreal forest ecosystems in Fennoscandia are characterized by disturbances such as forest fires, storms and floods. However, industrial forestry practices suppress forest fires and change the landscape, leading to a loss of habitats and associated species. Particularly lichen species with slow adaptation abilities and a strong substrate Specificity face an extinction debt in boreal forests since their substrates are long-lived but no longer created. In this study the species-substrate relationship of two red-listed forest-fire dependent lichen species Carbon-icola anthracophila and C.

Aim Developmental Coordination Disorder Questionnaire 2007 (DCDQ'07) is an international survey tool based on parents' estimation of their child's motor coordination skills. The purpose of this study was to investigate the validity of a Swedish version of DCDQ'07 by finding out how the classification of Developmental Coordination Disorder (DCD) via DCDQ'07 is consistent with the classification of DCD via the motor impairment test, Movement ABC.MethodIn the context of a larger study, a Swedish translated version of the parent survey DCDQ'07 were sent to 4000 randomly selected families with children aged 8 - 10 years in Stockholm County. The classifications "DCD" or "NOT DCD" with cut-off values ??of 56 (9 years) and 57 (10 years) were applied. Forty families were selected from 410 registered responses.

Aim Developmental Coordination Disorder Questionnaire 2007 (DCDQ'07) is an international survey tool based on parents' estimation of their child's motor coordination skills. The purpose of this study was to investigate the validity of a Swedish version of DCDQ'07 by finding out how the classification of Developmental Coordination Disorder (DCD) via DCDQ'07 is consistent with the classification of DCD via the motor impairment test, Movement ABC.MethodIn the context of a larger study, a Swedish translated version of the parent survey DCDQ'07 were sent to 4000 randomly selected families with children aged 8 - 10 years in Stockholm County. The classifications "DCD" or "NOT DCD" with cut-off values ??of 56 (9 years) and 57 (10 years) were applied. Forty families were selected from 410 registered responses.

AimTo validate the STOPP-8-questionnaire (S8Q), containing items from the SIH-questionnaire, against the criterion method accelerometry to estimate its capacity to assess 8 year old Swedish children?s physical activity (PA). Can it assess children?s physical activity? Can it differentiate the active children from the sedentary? If the answer is yes, how well can it do so? MethodData was collected during fall and winter the school year of 2008 - 2009 via the project STOPP-8. Out of 676 children at 12 schools in Stockholm area, 155 (boys n=72, girls n=83) provided complete questionnaire data and wore an accelerometer (Actiwatch 4) during 7 consecutive days which provided data for mean and median activity, time spent sedentary, in moderate to vigorous PA and vigorous PA.