Cyanobacteria belong to the oldest organisms of our planet. They use photosynthesis to produce ATP and gain biomass from carbon dioxide. The Cyanobacteria Nostoc punctiforme is a filamentous bacterium that consists of two different types of cells, vegetative cells and heterocysts. The type of cell it differentiates into depends on the media they grow in. In an ammonium-rich medium, the N.punctiforme consists of vegetative cells that differentiate into heterocysts when in the medium is changed to a low-concentration ammonium medium.

The Swedish boreal forests are limited by nitrogen (N) availability. While biological N2 fixation by Cyanobacteria hosted by pleurocarpous feather mosses are important sources of N input to natural boreal forest ecosystems, little is known about the patterns of N2 fixation in silvicultural systems. This study investigates the biological N2 fixation rates of two boreal feather moss species (Pleurozium schreberi and Hylocomium splendens) along a chronosequence from clear-cut to mature forest, as well as the vegetation characteristics along the chronosequence. Measurements and samples were collected from 32 forest sites that were classified into four age classes (clear-cut, pre-commercially thinned, thinned and mature forest). The sites were located near the city of Arvidsjaur in northern Sweden.