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5 Uppsatser om Ciprofloxacin - Sida 1 av 1
Studie av läkemedelssubstansers miljöfarliga egenskaper och effekt på miljö med fokus på ciprofloxacin, diklofenak och etinylestradiol
Läkemedel är en grupp kemikalier med effekt på olika fysiologiska processer hos människa och djur. Den största källa för läkemedelsrester är avloppsvatten från reningsverk. Eliminering av läkemedelssubtanser i reningsverk är ofta inte fullständig och läkemedelsrester i naturen har påvisats. Läkemedelsrester i vattenmiljö kan bioakumuleras och utgör toxisk risk för vattenlevande organismer. Med den globala utvecklingen av samhället förväntas exponeringen för läkemedelsrester öka.
Selektion av resistenta Escherichia coli vid låga antibiotikakoncentrationer i en gräsandsmodell
Enligt en erkänd hypotes, mutant selection window hypothesis, uppstår och upprätthålls antibiotikaresistens hos bakterier endast då antibiotikakoncentrationerna ligger över minimum inhibitory concentration (MIC)-värdet för en känslig, omuterad population. Det här skulle innebära att de låga antibiotikakoncentrationer man finner i miljön inte borde bidra till att upprätthålla resistens. Redan subinhibitoriska antibiotikakoncentrationer har dock visat sig ha effekt på bakterier genom att t.ex. öka mutationsfrekvensen hos bakterier, selektera för resistenta mutanter och ge upphov till nya sådana. Syftet med den här studien var att undersöka om en selektion vid låga antibiotikakoncentrationer sker också in vivo.
Prevalence and antibiotic resistance of Campylobacter spp. in poultry and raw meat in the Can Tho Province, Vietnam
The aims of the study were to investigate the prevalence and antibiotic resistance of Campylobacter jejuni and Campylobacter coli in chicken and chicken products, and to investigate farmer awareness of antibiotic resistance development. The study was conducted in the Can Tho province in Vietnam during six weeks in October to November 2008. Ninety-six samples from raw chickens were collected from twelve market places and analysed for the presence of Campylobacter. A total of 96 cloacal swabs from 20 farms were obtained and analysed for the presence of Campylobacter. Farmers were asked to answer eight questions concerning the housing of the chickens, modes of handling sick animals as well as use and knowledge of antibiotics and resistance development to antibiotics.
Cissus Quadrangularis Linn
The aims of the study were to investigate the prevalence and antibiotic resistance of Campylobacter jejuni and Campylobacter coli in chicken and chicken products, and to investigate farmer awareness of antibiotic resistance development. The study was conducted in the Can Tho province in Vietnam during six weeks in October to November 2008. Ninety-six samples from raw chickens were collected from twelve market places and analysed for the presence of Campylobacter. A total of 96 cloacal swabs from 20 farms were obtained and analysed for the presence of Campylobacter. Farmers were asked to answer eight questions concerning the housing of the chickens, modes of handling sick animals as well as use and knowledge of antibiotics and resistance development to antibiotics.
Antibiotic resistance associated with bacteria in irrigation water : a case study of irrigation ponds in Southern Sweden
The
focus
of
this
project
was
to
characterize
the
occurrence
of
ESBL-?producing
bacteria
in
two
irrigation
water
ponds
in
Southern
Sweden.
Samples
were
taken
from
two
ponds
nearby
each
other,
from
which
analyses
were
made
based
on
levels
of
community
and
individual
isolates.
Community
samples
were
used
for
attempts
for
characterization
of
resistance
patterns
in
irrigation
water
using
the
Omnilog
PM-?plate
system.
The
growth
of
community
microbiota
in
the
presence
of
the
following
antibiotic
substances
was
assessed;
amikacin,
gentamicin,
kanamycin,
penicillin
G,
ampicillin,
piperacillin,
trimethoprim,
potassium
tellurite
and
Ciprofloxacin.
Isolates
were
pure
cultured
using
semi-?selective
media:
LB,
Rainbow
Agar,
mEnterococcus,
0.1
TSA
and
VRBD.
A
total
of
177
isolates
were
isolated
randomly.
Each
isolate
was
identified
using
the
Omnilog
GENIII-?system
of
biochemical
characterization.
Further,
the
isolates
were
tested
on
Brilliance
ESBL-?agar
for
their
ability
to
grow.
Out
of
the
total
number
of
isolates,
30
were
selected,
preferably
Enterobacteriaceae
or
Pseudomonas
spp.,
based
on
their
high
similarity
to
library
strain
during
the
identification
in
the
Omnilog-?system.
These
were
further
characterized
using
Etest-?sticks.
Three
selected
strains
were
picked
for
further
analyses
using
exogenous
isolation
for
attempts
to
simulate
horizontal
gene
transfer,
and
Omnilog
PM-?panels
containing
various
antibiotics
of
interest.
Selections
of
samples
were
chosen
for
molecular
tests
targeting
plasmid-?borne
resistance
genes
CTX-?M1,
CTX-?M2
and
SHV.
The
method
for
assessing
community-?resistance
patterns
showed
ambiguous
data
that
was
difficult
to
interpret;
further
optimization
is
recommended.
For
all
tested
PM-?plates,
generally
greatest
differences
between
substances
were
found
among
wells
with
the
strongest
concentration
of
antibiotic
substance.
A
total
of
175
isolates
displayed
ability
to
grow
on
ESBL-?agar.
Etests
confirmed
ability
of
isolates
to
resist
many
?-?lactam
antibiotics
and
many
were
clinically
resistant
to
certain
substances.
No
plasmids
were
transferred
during
exogenous
isolation.
During
molecular
tests,
a
few
matches
were
found
for
the
CTX-?M1
gene.
Isolates
analyzed
with
Omnilog
PM-?plates
showed
resistance
to
most
compounds
tested.
The
isolates
showed
greatest
resistance
toward
penicillin
G,
ampicillin,
amikacin,
kanamycin
and
trimethoprim.
In
the
study,
it
was
established
that
resistance
among
bacteria
was
frequently
occurring
in
the
irrigation
water,
and
?-?lactamase
producing
bacteria
was
very
common.
Extended
studies
are
needed
to
assess
the
frequency-?
and
occurrence
of
horizontal
gene
transfer
(HGF)
in
this
environment.
Although
some
potential
corresponding
data
is
found,
it
is
at
this
point
not
possible
to
determine
whether
the
resistances
are
due
to
antibiotics
used
in
veterinary
medicine,
human
medicine
or
one
additional
reason
is
travel
and
trade.
in
irrigation
water
using
the
Omnilog
PM-?plate
system.
The
growth
of
community
microbiota
in
the
presence
of
the
following
antibiotic
substances
was
assessed;
amikacin,
gentamicin,
kanamycin,
penicillin
G,
ampicillin,
piperacillin,
trimethoprim,
potassium
tellurite
and
Ciprofloxacin.
Isolates
were
pure
cultured
using
semi-?selective
media:
LB,
Rainbow
Agar,
mEnterococcus,
0.1
TSA
and
VRBD.
A
total
of
177
isolates
were
isolated
randomly.
Each
isolate
was
identified
using
the
Omnilog
GENIII-?system
of
biochemical
characterization.
Further,
the
isolates
were
tested
on
Brilliance
ESBL-?agar
for
their
ability
to
grow.
Out
of
the
total
number
of
isolates,
30
were
selected,
preferably
Enterobacteriaceae
or
Pseudomonas
spp.,
based
on
their
high
similarity
to
library
strain
during
the
identification
in
the
Omnilog-?system.
These
were
further
characterized
using
Etest-?sticks.
Three
selected
strains
were
picked
for
further
analyses
using
exogenous
isolation
for
attempts
to
simulate
horizontal
gene
transfer,
and
Omnilog
PM-?panels
containing
various
antibiotics
of
interest.
Selections
of
samples
were
chosen
for
molecular
tests
targeting
plasmid-?borne
resistance
genes
CTX-?M1,
CTX-?M2
and
SHV.
The
method
for
assessing
community-?resistance
patterns
showed
ambiguous
data
that
was
difficult
to
interpret;
further
optimization
is
recommended.
For
all
tested
PM-?plates,
generally
greatest
differences
between
substances
were
found
among
wells
with
the
strongest
concentration
of
antibiotic
substance.
A
total
of
175
isolates
displayed
ability
to
grow
on
ESBL-?agar.
Etests
confirmed
ability
of
isolates
to
resist
many
?-?lactam
antibiotics
and
many
were
clinically
resistant
to
certain
substances.
No
plasmids
were
transferred
during
exogenous
isolation.
During
molecular
tests,
a
few
matches
were
found
for
the
CTX-?M1
gene.
Isolates
analyzed
with
Omnilog
PM-?plates
showed
resistance
to
most
compounds
tested.
The
isolates
showed
greatest
resistance
toward
penicillin
G,
ampicillin,
amikacin,
kanamycin
and
trimethoprim.
In
the
study,
it
was
established
that
resistance
among
bacteria
was
frequently
occurring
in
the
irrigation
water,
and
?-?lactamase
producing
bacteria
was
very
common.
Extended
studies
are
needed
to
assess
the
frequency-?
and
occurrence
of
horizontal
gene
transfer
(HGF)
in
this
environment.
Although
some
potential
corresponding
data
is
found,
it
is
at
this
point
not
possible
to
determine
whether
the
resistances
are
due
to
antibiotics
used
in
veterinary
medicine,
human
medicine
or
one
additional
reason
is
travel
and
trade..