An inflammatory reaction is induced after release of proinflammatory mediators such asinterleukin 1 and 6 and tumour necrosis factor ?. These mediators stimulate the liver tosuppress the syntheses of albumin and endure the syntheses of acute phase protein forinstance C-reactive protein. The aim of this paper was to perform a method validation on animmune turbidimetric assay to quantify C-reactive protein in Canine serum at the laboratory atSkara Animals Hospital, Skara, Sweden. The validation involved evaluation of the assaylinearity, precision, stability and recovery.The method was proved to be linear for both TruLab control and Medinor control.

Canine parvovirus is associated with two disorders: enteritis, which is common, and myocarditis, which is rare. Prominent clinical signs of parvovirus enteritis are vomiting, diarrhea and rapid dehydration. Canine parvovirus type 2 (CPV-2) is very contagious. When CPV-2 first emerged it had high rates of mortality, but later studies have shown that most infections are self-limiting and do not require veterinary care. Mortality rates vary greatly, and therefore it is important to determine risk factors for developing severe Canine parvovirus enteritis.

Malignant melanoma represents 4-7% of all Canine neoplasms and they are the most common malignant tumors of the oral cavity and digits. These tumors are often aggressive and metastasize quickly. To date there is no effective treatment and survival times are short. Most dogs are euthanized due to complications caused by metastases. Melanin pigment is a hallmark of melanomas and is usually present in large amounts in these tumors.

Two haematological instruments intended for small animal clinic use were evaluated at the Department of Clinical Chemistry, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden. The QBC-V and Vet abc were compared to a reference instrument, the Cell-Dyn 3500. Fresh blood samples were obtained from Canine and feline patients from the University Veterinary Hospital, SLU. The QBC-V was used for 111 Canine samples and 78 Canine blood samples were analysed with Vet abc. From cats, 23 samples were analysed with both instruments.

Canine herpes virus 1 (CHV-1) är en vitt utbredd smittsam patogen. Viruset tycks verka enzootiskt i hundpopulationer och ger upphov till en livslång latens hos infekterade djur. CHV-1 kan sedan reaktiveras vid exempelvis stress, som vid dräktighet. Hos vuxna hundar får infektionen ofta ett subkliniskt uttryck, men kan också ge upphov till respiratoriska symtom samt genitala blåsor och ögoninfektioner. Dräktiga tikar kan drabbas av fertilitetsproblem, med mumifikation, resorption, dödfödsel samt abort av foster.

The prime aim of this thesis was to investigate whether high or low iodine intake during certain periods of life was, or was not a strong risk factor for the development of Canine lymphocytic thyroiditis (CLT). And secondly, if a high or low selenium intake in a combination with high or low iodine intake also may have an influence on that risk factor. To investigate this, a dietary questionnaire was sent out to dog owners. The dogs were selected from an ongoing genetic study of CLT. The questions investigated brand and type of feed during different life stages (puppy, junior and adult) and also if there were any use of dietary supplements.

The aim of the study was to examine the retinal ON- and OFF-pathways of the Canine electroretinogram (ERG). The ON- and OFF-pathways are used to distinguish objects from their backgrounds. A dark object is discernible from a lighter background using the OFFpathway and the ON-pathway is used to descry a light object upon a darker background. This study shows that the dog ERG has both ON- and OFF-responses and that these responses can be separated by prolonging the flash duration over 50 ms. The dog OFF-response waveform was more similar to the cat OFF-response than the human OFF-response (Frishman, 2006; Zrenner and Gouras, 1979). In this study six female three-year-old Beagle dogs were included. Both background and flash stimuli were provided by LEDs (light emitting diodes) with a wavelength of 470 nm, a wavelength that is absorbed almost as effectively by the Canine M/L-cones as by the S-cones. These stimuli gave ERGs with both ON- and OFF-responses.

Oxytocin is a hormone which plays a crucial role in many reproductive and behavioural functions. It affects many organs and the classical peripheral targets are the mammary glands during lactation and uterus during labour. Oxytocin receptors have recently been described in a variety of normal tissues and primary cell cultures, but also in neoplastic tissues and established neoplastic cell lines, as breast cancer cell lines. The signalling pathways and biological effects of the oxytocin/oxytocin receptor system seem to depend on species, type of tissue, physiological versus neoplastic state and receptor location within the cell membrane.The aim of this project was to find out if the cell proliferation in the Canine mammary carcinoma cell line CMT-U27 was affected upon stimulation of oxytocin, and to investigate the presence of oxytocin receptors in these cells. The cell proliferation was examined by using an ELISA-kit, where the absorbance measured is relative to the amount of living cells.

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The development of quantitative, real-time PCR (qPCR) combined with the mapping of the Canine genome opens new possibilities in veterinary medicine. This method provides a quick and accurate quantification of the expression of a specific gene at a given point in time and thereby also information of how the gene expression for a certain protein is influenced by various conditions and diseases. One possible area of application is identifying bio markers for cancer. Recently the protein serglycin (the core protein of an intracellular proteoglycan) was found to act as a selective marker for the disease acute myeloid leukemia in humans (Niemann et al, 2007). Serglycin is produced by most hematopoietic cells, although mast cells account for the largest amount of serglycin.

Full-field flash electroretinography is an electrodiagnostic method used to examine the function of retinal cells. Light stimulation of the eye elicits electrical potentials in the retina. By aid of a corneal electrode and a reference electrode close to the back of the eye, the electrical potentials can be recorded and presented as an electroretinogram (ERG). ERGs driven by mainly one type of cones can be used to examine the function of a single cone class. In human beings, studies have shown the cone class sensitive to light of short wavelengths, the S-cones, to be more vulnerable to acquired damage than the other cone classes (Daley et al., 1987; Gouras et al., 1993; Greenstein et al., 1989). Dogs have two cone classes, S-cones, and M/L-cones (most sensitive to medium to long wavelengths).

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This article presents a retrospective case study combined with a review of the current literature concerning Canine gestational diabetes. A total of 14 cases have been collected from six different clinics in Sweden. There are few previously published reports on Canine gestational diabetes. Gestational diabetes is a disease that rarely occurs in pregnant bitches. The condition is due to the rise of progesterone that occurs during the estrus cycle.

Thymidine kinase 1 (TK1) is an intracellular enzyme involved in ?the salvage pathway? where the uncomplete parts of DNA are transformed into DNA precursors. The expression of TK1 is cell cykle specific, with the highest level during S-phase when DNA-synthesis is most active. One kind of TK1 is present in serum and an increase in TK1 activity in serum is due to either the level of DNA synthesis in the body or the number of cells dying in a state of replication. This can be used and is used as a marker for prognosis and evaluation of treatment in human patients with leukemia and lymphoma.

The aim of this study was to describe how body language of humans affects the domestic dog (Canis familiaris) and how it can be implanted in the stressed situation that occur at an animal hospital. The subject is brought to notice because the author has experienced that dogs can get more stressed when a stressed and nervous owner is attending. Humans affect each other through posture, facial expression, voice and movement, and are often driven by their emotions, which can differentiate the verbal communication from the non-verbal communication. This occurs both consciously and unconsciously. Every human uses body language but not everyone knows how it is used or how to understand it.