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Antibiotic resistance associated with bacteria in irrigation water : a case study of irrigation ponds in Southern Sweden
The
focus
of
this
project
was
to
characterize
the
occurrence
of
ESBL-?producing
bacteria
in
two
irrigation
water
ponds
in
Southern
Sweden.
Samples
were
taken
from
two
ponds
nearby
each
other,
from
which
analyses
were
made
based
on
levels
of
community
and
individual
isolates.
Community
samples
were
used
for
attempts
for
characterization
of
resistance
patterns
in
irrigation
water
using
the
Omnilog
PM-?plate
system.
The
growth
of
community
microbiota
in
the
presence
of
the
following
antibiotic
substances
was
assessed;
amikacin,
gentamicin,
kanamycin,
penicillin
G,
ampicillin,
piperacillin,
trimethoprim,
potassium
tellurite
and
ciprofloxacin.
Isolates
were
pure
cultured
using
semi-?selective
media:
LB,
Rainbow
Agar,
mEnterococcus,
0.1
TSA
and
VRBD.
A
total
of
177
isolates
were
isolated
randomly.
Each
isolate
was
identified
using
the
Omnilog
GENIII-?system
of
biochemical
characterization.
Further,
the
isolates
were
tested
on
Brilliance
ESBL-?agar
for
their
ability
to
grow.
Out
of
the
total
number
of
isolates,
30
were
selected,
preferably
Enterobacteriaceae
or
Pseudomonas
spp.,
based
on
their
high
similarity
to
library
strain
during
the
identification
in
the
Omnilog-?system.
These
were
further
characterized
using
Etest-?sticks.
Three
selected
strains
were
picked
for
further
analyses
using
exogenous
isolation
for
attempts
to
simulate
horizontal
gene
transfer,
and
Omnilog
PM-?panels
containing
various
antibiotics
of
interest.
Selections
of
samples
were
chosen
for
molecular
tests
targeting
plasmid-?borne
resistance
genes
CTX-?M1,
CTX-?M2
and
SHV.
The
method
for
assessing
community-?resistance
patterns
showed
ambiguous
data
that
was
difficult
to
interpret;
further
optimization
is
recommended.
For
all
tested
PM-?plates,
generally
greatest
differences
between
substances
were
found
among
wells
with
the
strongest
concentration
of
antibiotic
substance.
A
total
of
175
isolates
displayed
ability
to
grow
on
ESBL-?agar.
Etests
confirmed
ability
of
isolates
to
resist
many
?-?lactam
antibiotics
and
many
were
clinically
resistant
to
certain
substances.
No
plasmids
were
transferred
during
exogenous
isolation.
During
molecular
tests,
a
few
matches
were
found
for
the
CTX-?M1
gene.
Isolates
analyzed
with
Omnilog
PM-?plates
showed
resistance
to
most
compounds
tested.
The
isolates
showed
greatest
resistance
toward
penicillin
G,
ampicillin,
amikacin,
kanamycin
and
trimethoprim.
In
the
study,
it
was
established
that
resistance
among
bacteria
was
frequently
occurring
in
the
irrigation
water,
and
?-?lactamase
producing
bacteria
was
very
common.
Extended
studies
are
needed
to
assess
the
frequency-?
and
occurrence
of
horizontal
gene
transfer
(HGF)
in
this
environment.
Although
some
potential
corresponding
data
is
found,
it
is
at
this
point
not
possible
to
determine
whether
the
resistances
are
due
to
antibiotics
used
in
veterinary
medicine,
human
medicine
or
one
additional
reason
is
travel
and
trade.
in
irrigation
water
using
the
Omnilog
PM-?plate
system.
The
growth
of
community
microbiota
in
the
presence
of
the
following
antibiotic
substances
was
assessed;
amikacin,
gentamicin,
kanamycin,
penicillin
G,
ampicillin,
piperacillin,
trimethoprim,
potassium
tellurite
and
ciprofloxacin.
Isolates
were
pure
cultured
using
semi-?selective
media:
LB,
Rainbow
Agar,
mEnterococcus,
0.1
TSA
and
VRBD.
A
total
of
177
isolates
were
isolated
randomly.
Each
isolate
was
identified
using
the
Omnilog
GENIII-?system
of
biochemical
characterization.
Further,
the
isolates
were
tested
on
Brilliance
ESBL-?agar
for
their
ability
to
grow.
Out
of
the
total
number
of
isolates,
30
were
selected,
preferably
Enterobacteriaceae
or
Pseudomonas
spp.,
based
on
their
high
similarity
to
library
strain
during
the
identification
in
the
Omnilog-?system.
These
were
further
characterized
using
Etest-?sticks.
Three
selected
strains
were
picked
for
further
analyses
using
exogenous
isolation
for
attempts
to
simulate
horizontal
gene
transfer,
and
Omnilog
PM-?panels
containing
various
antibiotics
of
interest.
Selections
of
samples
were
chosen
for
molecular
tests
targeting
plasmid-?borne
resistance
genes
CTX-?M1,
CTX-?M2
and
SHV.
The
method
for
assessing
community-?resistance
patterns
showed
ambiguous
data
that
was
difficult
to
interpret;
further
optimization
is
recommended.
For
all
tested
PM-?plates,
generally
greatest
differences
between
substances
were
found
among
wells
with
the
strongest
concentration
of
antibiotic
substance.
A
total
of
175
isolates
displayed
ability
to
grow
on
ESBL-?agar.
Etests
confirmed
ability
of
isolates
to
resist
many
?-?lactam
antibiotics
and
many
were
clinically
resistant
to
certain
substances.
No
plasmids
were
transferred
during
exogenous
isolation.
During
molecular
tests,
a
few
matches
were
found
for
the
CTX-?M1
gene.
Isolates
analyzed
with
Omnilog
PM-?plates
showed
resistance
to
most
compounds
tested.
The
isolates
showed
greatest
resistance
toward
penicillin
G,
ampicillin,
amikacin,
kanamycin
and
trimethoprim.
In
the
study,
it
was
established
that
resistance
among
bacteria
was
frequently
occurring
in
the
irrigation
water,
and
?-?lactamase
producing
bacteria
was
very
common.
Extended
studies
are
needed
to
assess
the
frequency-?
and
occurrence
of
horizontal
gene
transfer
(HGF)
in
this
environment.
Although
some
potential
corresponding
data
is
found,
it
is
at
this
point
not
possible
to
determine
whether
the
resistances
are
due
to
antibiotics
used
in
veterinary
medicine,
human
medicine
or
one
additional
reason
is
travel
and
trade..
Att tala om ansvar : om Levinas ansvarsbegrepp i gränslandet mellan det etiska Sägandet och det politiskt Sagda
The present essay concerns the Levinasian understanding of responsibility in relation to the two spheres that Levinas introduces in his second major work Otherwise than Being or Beyond Essence, namely the Saying and the Said. The essay investigates some of the different meanings that the concept responsibility takes on in regard to these two spheres, and thus also in regard to the separation between the ethical and the political that is apparent in the book. Is it possible to find a politically uttered responsibility in Levinas Otherwise than Being or Beyond Essence?The essay wants to emphasize the continuous transition between the ethical or the Saying and the political or the Said, and vice versa, with the purpose of showing how the two spheres may be conceived simultaneously. By posing the relationship between the two as a reciprocal rather than a oppositional one, the essay aims to suggest that an understanding of responsibility at once must be ethical and political.
Idrottslärares syn på kamratbedömning inom ämnet idrott och hälsa i grundskolan
AimThe aim of the study is to gain insight into how physical education teachers reflect on peerassessment in Physical Education (PE). The study was limited to investigate teachers? conception of peer assessment. The study also examines whether teachers can describe anymethods of peer assessment that they use or have used in their teaching, and what strategic choices they talk about when they describe their methods.MethodQualitative interviews were used to collect data. The interviews can be referred to as in-depthinterviews with a particular focus on concepts.
Tidskriftsrummet och integration: en kvalitativ undersökning av tidningsläsandet hos några individer av första generationens invandrare
The purpose of this master?s thesis is to find out in which ways a group of first generation immigrants use Swedish papers and magazines in a public library and which information needs this group has about Swedish society. One important aspect in this essay is integration of immigrants in the Swedish society as a possible effect of newspaper reading. The method used in this study is qualitative. Data is generated through semi-structured interviews.
The Sad Story of Regulated Markets: A study of relationships and networks on the Swedish market of alcoholic beverages
The purpose of this thesis is to create a greater understanding of relationships and networks on regulated markets. This includes what the climate are like on such a market and how different aspects within relationships and networks are affected by regulations. By the use of a deductive approach, a qualitative study was performed. This was carried out by interviewing different actors on the Swedish market of alcoholicbeverages. The theoretical framework includes relationship quality, consisting of power and dependence, cooperation,adaptation and trust.
En barnomsorg som inte räcker till : En undersökning om hur nattis framställs i media
This is an examination that investigates how daycare during nights, evenings and weekends, so called ?nattis?, is produced in media with restriction to three big Swedish newspapers during 2004-2012. The main intention and the base to this work is to see how media chooses to produce daycare during nights. As a help to find a result to this main intention there are four questions. These questions are which similarities and differences that can be shown in the articles, which main arguments that can be shown, if it?s a positive or negative picture that the articles are producing and which patterns and themes that can be shown. The method to find an answer to these questions and the main intention were an investigation of 21 articles.
Ut ur hedersförtryck : En studie baserad på sju självbiografier skrivna av kvinnor som levt under hedersförtryck.
The aim of this study is to understand which strategies helped women escape honourbased violence, by reading the autobiographies of seven women. Honourbased violence is a relatively new problem in Sweden, but it has existed for a long time. According to FN (2012) 5000 women are murdered every year in the name of honour. Honourbased violence is about maintaining the honour of the family. Womens sexuality is connected to men´s honour, and because of that they are not allowed to express it.
Lönsamhetsbedömning av utökad MR-kapacitet på Karolinska Universitetssjukhuset
This thesis analyses how a radiology department at the largest hospital in Sweden, Karolinska University Hospital, most profitably could expand its capacity for investigating cancer patients using magnetic resonance imaging (MRI). The queues today for patients waiting to be scanned are long, which delays the start of cancer treatments. Meanwhile the hospital has a budget deficit of half a billion SEK, which make any expansion hard. In this thesis we evaluate the alternatives of investing in new machinery; utilize the current machinery better by using them more days annually or through a shift work system. Finally we evaluate a combination of these.
Storytelling ur ett användarperspektiv : Vilka faktorer styr upplevelsen
Denna uppsats baseras på en undersökning där en jämförelse gjorts mellan serie- och filmformatet. Syftet med denna studie var att undersöka om kombinationen av bild och ord i serieformat kan användas som informationsbärare för till exempel elektroniska medier. Samt att undersöka vilka faktorer som påverkar användarens upplevelse utifrån en presentation via serie- ochfilmformatet. Frågor som ställs i studien är hur serieformatet upplevs som bärare i kommunikationssyfte, och vilka faktorer som påverkar användarens upplevelse av serie- och filmformatet.För studien valdes en kvalitativ metod i form av semistrukturerade intervjuer. För den jämförelse undersökningen baserades på valdes en instruktionsfilm som visar hur en hjärtstartare fungerar.
Sex differences in feeding visit rates in Northern Wheatears Oenanthe oenanthe
AbstractThe Northern Wheatear (Oenanthe oenanthe) is a small insectivorous passerine bird living in open landscapes. Both parents feed the brood of approximately five chicks, but little is known about how each sex allocates their feeding effort. In this study I investigate feeding nest visit differences between male and female Northern Wheatears relative to the age of the chicks, the number of chicks and the quality of the territory for 17 pairs from 2008-2010. Data were collected using automated data loggers to record nest visitation rates during chick feeding, and the sex was determined from nest video cameras. I also examine if the parents? rate of feeding visits changes over the nestling period.
Ibn Khaldun om Banu Umayya : Historieskrivningen om det umayyadiska kalifatet och dess återgivelse i al-Muqaddima
The history of Banu Umayya has since the collapse of the caliphate occupied a major part in Islamic historiography. The present thesis analyses the presentation of the Umayyads in Ibn Khaldun?s al-Muqaddima and its relation to previous historical traditions. The thesis examines the social and intellectual context in which Ibn Khaldun stood and how it is represented in his historiography, while also providing an overview of the various socio-political, intellectual and historiographical developments in Islam. The theoretical perspectives are based on the concept of agency, examining the intellectual room for manoeuvre that the historian disposed of while composing the works.
Nätmobbning Om relationen mellan traditionell mobbning och nätmobbning i grundskolan
This study investigates the relation between cyberbullying and traditional bullying (in school). In previous research it has been argued that one should separate cyberbullying and traditional bullying both in definitional and measurements issues. However, in Swedish school, there are no marked differences between the two forms of bullying, teachers are responsible to prevent them both. The research is based on 3925 students in years 3-9 (1950 girls and 1975 boys) in 19 schools in the Gothenburg region and two from the Stockholm region. The students have taken a digital survey at school, regarding both traditional and cyber bullying.
IT system för vägtransporter och tillhörande terminalverksamhet
We have chosen to write our thesis within a transport company. The work was carried out at DHL activities in Uppsala. DHL is one of the leading transport companies worldwide. Our interest lies in more efficient and advanced transportation and terminals, as globalization and new trends require effective solutions.The aim of our work is to explain the need for information technology applications in road haulage and associated terminal operations. Furthermore, we analyses terminal and applied information technology.
Lönsamhet och råd i renoveringen av miljonprogrammet
The real estate industry is a sector where the companies generally have a capital structure which is high leveraged. The financing? with the related terms ? is therefore specifically of high importance for the companies in the sector. Traditionally, the way of obtain financing is by borrowing from the bank. Lately, due to new bank regulations, the banks have become more restrictive in their lending which have lead to a growth of other financing alternatives.
Manliga vampyrer och kvinnliga offer. En diskursanalys av det övernaturliga och könsroller i The Vampire Diaries
The purpose of this essay is to examine how the supernatural and the occult are portrayed inpopular culture. To be able to do this, I have chosen to perform a discourse analysis of the twofirst novels in the L. J Smith series The Vampire Diaries. The novels are called TheAwakening and The Struggle.I apply the following questions in my analysis to narrow down the perspective further.?In what situations are supernatural used in the books and what part does it play??How does the supernatural affect the main characters and how is this portrayed in the novel??How is the supernatural portrayed depending on the gender of the character?I will focus mostly on the vampires since they generally are the ones who introduce thesupernatural elements into the story.I am applying gender theory in my discourse analyses of the novels, to hopefully be able togive a wider picture of the supernatural in relation to gender in the novels.I begin with analyzing the main discourses in the story, revolving around the supernatural.Secondly I analyze what impact the supernatural has on the main character.