The focus of this project was to characterize the occurrence of ESBL-­?producing bacteria in two irrigation water ponds in Southern Sweden. Samples were taken from two ponds nearby each other, from which analyses were made based on levels of community and individual isolates. Community samples were used for attempts for characterization of resistance patterns in irrigation water using the Omnilog PM-­?plate system. The growth of community microbiota in the presence of the following antibiotic substances was assessed; amikacin, gentamicin, kanamycin, penicillin G, ampicillin, piperacillin, trimethoprim, potassium tellurite and ciprofloxacin. Isolates were pure cultured using semi-­?selective media: LB, Rainbow Agar, mEnterococcus, 0.1 TSA and VRBD. A total of 177 isolates were isolated randomly. Each isolate was identified using the Omnilog GENIII-­?system of biochemical characterization. Further, the isolates were tested on Brilliance ESBL-­?agar for their ability to grow. Out of the total number of isolates, 30 were selected, preferably Enterobacteriaceae or Pseudomonas spp., based on their high similarity to library strain during the identification in the Omnilog-­?system. These were further characterized using Etest-­?sticks. Three selected strains were picked for further analyses using exogenous isolation for attempts to simulate horizontal gene transfer, and Omnilog PM-­?panels containing various antibiotics of interest. Selections of samples were chosen for molecular tests targeting plasmid-­?borne resistance genes CTX-­?M1, CTX-­?M2 and SHV. The method for assessing community-­?resistance patterns showed ambiguous data that was difficult to interpret; further optimization is recommended. For all tested PM-­?plates, generally greatest differences between substances were found among wells with the strongest concentration of antibiotic substance. A total of 175 isolates displayed ability to grow on ESBL-­?agar. Etests confirmed ability of isolates to resist many ?-­?lactam antibiotics and many were clinically resistant to certain substances. No plasmids were transferred during exogenous isolation. During molecular tests, a few matches were found for the CTX-­?M1 gene. Isolates analyzed with Omnilog PM-­?plates showed resistance to most compounds tested. The isolates showed greatest resistance toward penicillin G, ampicillin, amikacin, kanamycin and trimethoprim. In the study, it was established that resistance among bacteria was frequently occurring in the irrigation water, and ?-­?lactamase producing bacteria was very common. Extended studies are needed to assess the frequency-­? and occurrence of horizontal gene transfer (HGF) in this environment. Although some potential corresponding data is found, it is at this point not possible to determine whether the resistances are due to antibiotics used in Veterinary medicine, human medicine or one additional reason is travel and trade. in irrigation water using the Omnilog PM-­?plate system. The growth of community microbiota in the presence of the following antibiotic substances was assessed; amikacin, gentamicin, kanamycin, penicillin G, ampicillin, piperacillin, trimethoprim, potassium tellurite and ciprofloxacin. Isolates were pure cultured using semi-­?selective media: LB, Rainbow Agar, mEnterococcus, 0.1 TSA and VRBD. A total of 177 isolates were isolated randomly. Each isolate was identified using the Omnilog GENIII-­?system of biochemical characterization. Further, the isolates were tested on Brilliance ESBL-­?agar for their ability to grow. Out of the total number of isolates, 30 were selected, preferably Enterobacteriaceae or Pseudomonas spp., based on their high similarity to library strain during the identification in the Omnilog-­?system. These were further characterized using Etest-­?sticks. Three selected strains were picked for further analyses using exogenous isolation for attempts to simulate horizontal gene transfer, and Omnilog PM-­?panels containing various antibiotics of interest. Selections of samples were chosen for molecular tests targeting plasmid-­?borne resistance genes CTX-­?M1, CTX-­?M2 and SHV. The method for assessing community-­?resistance patterns showed ambiguous data that was difficult to interpret; further optimization is recommended. For all tested PM-­?plates, generally greatest differences between substances were found among wells with the strongest concentration of antibiotic substance. A total of 175 isolates displayed ability to grow on ESBL-­?agar. Etests confirmed ability of isolates to resist many ?-­?lactam antibiotics and many were clinically resistant to certain substances. No plasmids were transferred during exogenous isolation. During molecular tests, a few matches were found for the CTX-­?M1 gene. Isolates analyzed with Omnilog PM-­?plates showed resistance to most compounds tested. The isolates showed greatest resistance toward penicillin G, ampicillin, amikacin, kanamycin and trimethoprim. In the study, it was established that resistance among bacteria was frequently occurring in the irrigation water, and ?-­?lactamase producing bacteria was very common. Extended studies are needed to assess the frequency-­? and occurrence of horizontal gene transfer (HGF) in this environment. Although some potential corresponding data is found, it is at this point not possible to determine whether the resistances are due to antibiotics used in Veterinary medicine, human medicine or one additional reason is travel and trade..

Wounds on horses are common and sometimes difficult to treat. The wounds are often big and could be discovered too late for primary sutures, which have to be applied within 8 hours from time of accident, before bacteria has been able to infiltrate the surrounding tissue. The healing is often complicated by delayed healing and wound infection with consequences like phlegmone, fistulation and proud flesh formation.To avoid these consequences, the traditional practice has been to administer systemic antibiotics alone, systemic antibiotics in combination with topical antibiotics or topical antibiotics alone. Because few antibiotics can be used in horses, is it important to household with the few substances that are available. In Veterinary medicine today, the increasing resistance amongst pathogenic microbes such as MRSA presents a growing problem.

Animal welfare in Sweden has gone through major changes during the later years. In 2004, Swedish Animal Welfare Agency took over the central responsibility for the animal welfare from the Swedish Board of Agriculture, and in 2007 the board again became the central authority for animal welfare in Sweden.Even the practical supervision has changed, from the 1 of January 2009 the supervision is to be performed by the county administrative boards, instead of by the individual municipalities. Since 2006 every horse in the European Union has to have an identification card, in Sweden called a ?horse passport? which describes the color and markings of the horse as well as any Veterinary treatments.The ELOF-project, a collaborative project between the Swedish Board of Agriculture and the county administrative boards, intends to make the animal welfare supervision effective, standardised, fair and risk-based. Because of the takeover of the supervision and of the ELOF-project the Örebro County Administrative Board has began to make an inventory of the number of horses and horsefarms in the county. Another Ethology and Animal Welfare-student and I did, in collaboration with the county administrative board created a checklist that in connection with the inventory will lead to a risk-based animal welfare supervision.

Extensive cattle management was reintroduced in Sweden during the 1950´s and 60´s. Usually this term refers to suckler cows for meat production that is kept outdoors all year around. Suckler cow farms tend to become fewer but bigger over time. Still however most of the farms with beef cows are small. Extensive animal husbandry can lead to animals becoming shy which leads to difficulties when they are handled due to Veterinary treatments, marking, transport etc.

Hydrotherapy is a relatively new area when it comes to treatment and prevention of different problems in dogs, but an area that is growing in popularity. In Sweden, the person who performs hydrotherapy on dogs doesn?t need Veterinary education, which is the case in some other countries. What makes hydrotherapy advantageous is that water has an ability to provide an environment where injured body parts can be supported, which means that rehabilitation can start early without the risk of affecting the injury and in the same time relieve the pain for the patient. There are only a few studies of hydrotherapy on dogs and these studies focus on the physical health of the dog.

Two haematological instruments intended for small animal clinic use were evaluated at the Department of Clinical Chemistry, Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden. The QBC-V and Vet abc were compared to a reference instrument, the Cell-Dyn 3500. Fresh blood samples were obtained from canine and feline patients from the University Veterinary Hospital, SLU. The QBC-V was used for 111 canine samples and 78 canine blood samples were analysed with Vet abc. From cats, 23 samples were analysed with both instruments.

Staphylococcus pseudintermedius (S. pseudintermedius) är en opportunist som ingår i hundars normalflora och den bakterie som oftast orsakar pyodermi hos hund. En alltför frikostig användning av antibiotika inom veterinärmedicinen har selekterat fram nya resistenta bakterier som meticillinresistenta S. pseudintermedius (MRSP). Det är en bakterie som i vissa fall visat sig vara resistent mot alla större antibiotikagrupper som används inom veterinärmedicinen.

SAMMANFATTNING Detta examensarbete syftar till att kvalitetssäkra omvårdnaden av hästar med medicinsk kolik på djursjukhusens stationärvårdsavdelning. Genom att bidra med ett förslag till standardvårdplan för hästar med medicinsk kolik vill vi optimera hästens vård och omvårdnad under sjukhusvistelsen. Syftet med att arbeta med standardvårdplaner (SVP) är att skapa högkvalitativ omvårdnad, underlätta dokumentationsarbetet och i förlängningen kunna få mer tid till att se och vårda den enskilde patienten. Kolik är en åkomma med många olika samverkande faktorer, både miljöbetingade och eventuellt ärftliga Kolik i matsmältningskanalen delas ofta in i medicinsk- och kirurgisk kolik och i detta arbete läggs vikten på medicinsk kolik. Huvuddelen (~90 %) av all kolik kräver ingen kirurgi och tillhör därmed gruppen medicinsk kolik. Kolikavsnittet i arbetet är tänkt som en kunskapsöversikt över sjukdomstillståndet och ska användas som ett styrdokument för att trygga en kvalitativ basnivå på omvårdnaden. I standardvårdplanen har ett antal riskområden identifierats och därefter har riskdiagnoser, mål och åtgärder för att uppnå huvudmålet - en kolikfri patient, utarbetats. Därpå utvecklades kryssprotokoll för parametrar vid observation av kolikhäst och för smärtbedömning vid medicinsk kolik. Nyckelord: dokumentation, medicinsk kolik, omvårdnad, standardvårdplan SUMMARY This diploma work aims to assure the quality care of horses with medical colic on the Veterinary hospital's stationary ward.

Hemoplus® (Sarstedt, Nümbrecht, Tyskland) is a guaiac-based fecal occult blood test developed for human medicine. Guaiac-based tests make use of the pseudoperoxidase activity of hemoglobin so the test is not specific for human hemoglobin and can therefore be used in Veterinary medicine. Peroxidase-containing foods such as meat (hemoglobin and myoglobin) can cause false-positive results of the test. To ensure that a positive result is not caused by diet, it has been suggested that canine patients should be fed a meatless diet for a few days prior to fecal occult blood testing. The aim of this study was to evaluate the effect of commercial dog food on guaiac-based tests. In this study fecal samples from healthy dogs were analyzed with Hemoplus®.

Reasons for performing this study: Colic has for a long time been seen as a serious health problem in the equine population. Even so, the knowledge of colic seems to differ a great deal among the people interacting with horses. A large number of studies have for several years looked at risk factors that cause colic, in order to provide knowledge about them. The knowledge can then be used as tools for developing prophylactic methods in order to reduce the number of horses that develop colic. Objectives The aim with this study was to investigate if people have the correct knowledge about colic in horses, which risk factors that cause colic today and describe prophylactic methods that can result in avoidance of colic in horses. Method Eleven Veterinary practices were contacted in order to send out two questionnaires for a study of six weeks.

Sambandet mellan våld mot djur och våld i nära relationer är väl dokumenterat. Forskning har visat att en person som misshandlar sin partner kan använda djuren i hemmet som ett medel för att skrämma, kontrollera, hämnas och utsätta sin partner för tvång. Det kan ske genom hot att skada eller döda djuret och faktiska handlingar där personen ifråga gör det. Våld mot djur är också förknippat med eskalerande våld. Studier tyder på att djurens utsatthet gör det svårare för den våldsutsatta partnern att lämna förhållandet, då hen är orolig för vad som ska hända med djuret.

The virus-mediated disease Rift Valley fever (RVF) was discovered during an outbreak in Kenya in the 1930s. Since then it has spread to most parts of Sub-Saharan Africa, and in the last decades several outbreaks have caused economic and health issues in Africa, Yemen and Saudi-Arabia. The disease mainly affects domestic livestock, causing abortions, but is also a zoonosis. To be able to control the spread of the disease it is important with surveillance for better knowledge about the distribution and virus circulation even in inter-epidemic/epizootic periods. The human population is growing, and people and their livestock constantly move closer to areas with wild animals which act as reservoirs for different viruses. Also, humans and their animals often get within reach for arthropod vectors, hosting or carrying viruses.

Aggression between laboratory mice is a common problem and can result in welfare issues such as mice hurting or killing each other and also stress. Swedish laws and regulations clearly states that mice must be housed with a companion due to their natural social structure. Many studies have investigated the effect off enrichment, group size, cage size and other potential changes in in their environment on the occurrence of aggression. Even with these changes aggression can exist and therefore a new solution was tested in this paper. The proposed solution was to house an intact mouse with a castrated one and thus fulfill laws and regulations while still maintaining the physiology of one of the mice for the purpose of medical experiments.

Equine metabolic syndrome (EMS) it?s a clinical condition that includes obesity, regional adiposity, insulin resistance (IR) and laminitis. A similar syndrome exists in human medi-cine, the metabolic syndrome (MS), were systemic hypertension also is a prominent fea-ture. The systemic hypertension seen in humans with MS is partly due to a vascular endo-thelial dysfunction with an enhanced endothelin-1 (ET-1) mediated vasoconstriction. There are indications that systemic hypertension might be a feature in EMS as well but if ET-1 is involved in the development of the systemic hypertension in horses is not known.

Mastitis is the most common disease of dairy cows. Mastitis can be painful for the dairy cow and causes economical losses for the farmer. These losses are due to decreased milk production, more working hours for the farmer, treatment costs and possible costs due to culling of the cow. The udder inflammation, mastitis, is often caused by bacteria infection. One of the most common bacteria found to cause mastitis in Sweden is Staphylococcus aureus (S.