An attempt was made to GFP-tag the effector proteins of AvrLm6 and AvrLm4-7 using the GFP-tagging gene knockout method (GGKO) developed by Saitoh et al. (2008) in order to determine whether or not they are secreted. Successful pETHG-(target)KO vectors were not generated. The protocol was examined for potential errors. Fatal errors were pinpointed to the ligation reaction and the transformation required to generate and propagate the desired vector pETGH-(target)KO.

We are always surrounded by space, not just only in a room with four walls, but also when we are outdoors.Constantly we move in a flow of space that changes whenever we move. Unaware we are affectedby this threedimensional surrounding of ours, and it reacts with our perception. Many times, we chooseto translate it into something two-dimensional, for example when we take a photo, recording a movie orframing a view with a window. It helps us to catch a moment, a piece of reality and restore it in our ownway. In this project I?ve chosen to explore this process, to interpret and create my own theories about thistranslation from space to a two-dimensional surface.

The objective of this master thesis was to study the relation between public green open space and health among adolescents in Uppsala municipality, Sweden. The aim of the study was to investigate if there is an association between the access to green open space in the living environment and adolescent´s health, in terms of fysical activity, self- reported health and overweight. The public health questionnaire ?Liv och Hälsa Ung 2009? provided data together with a map over public green space, where the measure taken into account is the proportion of public green space in the living environment. The study includes 3423 adolescents, 12-19 years old.

Market capitalism sets the conditions for how the world is conceived, experienced and organized at various spatial scales (global to local). Currently, competitiveness, entrepreneurship and economic growth are key values that shape how the world is conceived, experienced and organized. Discourses of place marketing, place branding, and the construction of places as (economic) products, are part and parcel of the heightened commodification of space. Tourism has been a major factor in the commodification of place and space. More often than not, tourism has been seen as a possibility for peripheral places, in their struggle finding new ways towards development.

The paper evaluates two housing proposals with Space syntax as method. The proposals are very different in a topological sense but are in a configural sense almost the same. This effect is because both proposals connect themselves to the existing configural structure of the city in the same way. Neither of them takes advantages of the most integrated streets in the existing structure and use them to concentrate pedestrian and car flows to the streets or points which are intended to be the most urban areas. The knowledge of Space syntax and this evaluation puts focus on the pour understanding among architects and planners of what effect urban design proposals will have..

This essay investigates the function of fictional space and thematics in Line Knutzon?s dramatic works.My main theoretical concept is thematic unity, factors that unite several plays by one author.I have found three major themes: the encounter, identity and childhood, which I study in five of Knutzon?s plays: The Splint In The Heart, The Latest Thing, First You?re Born, The Air Others Breathe and The Time Will Soon Come.I study three aspects of space: fictive space, where the play is set, the characters? inner space and exterior space, that which is outside fictive space: society. Fictive space is shown mainly via dialogue and context.It is evident that the themes, most clearly visible in the five chosen plays, are very important in Line Knutzon?s dramatic works. Most typical is the way she portrays childhood memories, in monologues showing that her characters exist in a ?hole?, in the void between childhood and adulthood.My investigation leads to the conclusion that Knutzon deals with existential matters: there is a kind of constant chaos, within her characters, expressed in actions and thoughts.

ABSTRACTToday's modern technological society is constantly evolving and house planning is one area that is constantly creating new technical solutions. By looking back at selected architectural eras during the 1900?s, the idea is to study and analyse three building structures in Gothenburg with a focus on quality and content in the urban space.In order to provide a background for the three selected areas, their history has been studied. The main question for the research is: how do different building structures affect the content and qualities in the urban space?The areas have been selected to represent three typical epochs in Swedish building history.

This report studies how the divergence between men and women is manifested in the physical space and how the representation of room recreates this divergence. Spaces are coded and women have traditionally been associated with private space and men with public space, leading to that the home is encoded as female space and public squares as male space. Thereby women lack a natural place in public space. The National Board of Housing, Building and Planning (Boverket) wants to change this situation by enforcing safety-creating measures based on gender equality theories.I have studied what potential risks the project initiated by Boverket as well as discuss the potential problems with representation. Can representation be used without freezing the space? I also search for methods for working with a gender perspective without reproducing gender stereotypes and roles and avoiding encoding the private as female and public as male? Also I use a public seminar with urban planners, artists and architects to retrieve how we work with these issues today.I conclude that city planning needs to incorporate new strategies of thought that does not divide space, e.g.

This project in an in depth exploration about the basics of creating space in the form of an experimental investigation using my own perceptions as reference. Within a method, especially outlined for the project using descriptive words of opposites, my interpretation of the words has been formed into models of rooms. Four workshops with restrictions and rules acted as a platform for the striving towards the aim of a wider understanding of how space can be experienced. Through analysis I have formulated the different parameters influencing space itself and my perceptions of it. During the project a language for visualizing the interpretations of a room has been further evolved.

Purpose: The purpose of the study is to gain a deeper understanding of influencing behaviours concerning individualized viewing in relation to VoD-services (Video on Demand), and how this reflects the perceived viewing behaviour in terms of habits, content and social actions.Theory: The theory that primarily underpins this study is Jacob Bjur?s thesis Transforming Audiences. Bjur?s thesis provides an analytical model of individualized viewing behaviour that has been used in this study case. The model is founded on three central tenet areas of individualization, Habitualness that ensures habits, Socialness that refers to the social space and Referential space that ensures content/selection and viewership as a referential act.Method/Material: The study is based on a qualitative research method underpinned by focus groups interviews.Results: The research demonstrates strong tendencies towards an individualised viewing behaviour in the use of VoD-services.

The aim of this study was to develop a simple model for calculating stock costs for bio fuel at Billerud Karlsborg and subsequently recommending a suitable stock level of bio fuel. The stock components considered in the model are GROT (branches and tops), bark, dry wood chips, raw wood chips from barking and sawdust. The calculations are based on a probability distribution of historical deviations between ordered and delivered volumes. The model presents two different ways to manage the supply in case of irregular deliveries. The first way is to stock enough volume to cover the deviations and the second way is to compensate with a quick purchase from the spot market (at extra high purchase prices).

Internet and media have led to companies' operations becoming more visible and transparent to the public during the 21st century. Several notable corporate scandals have led to society placing greater demands on businesses to assume more accountability for their actions. This study aims to examine and develop existing research regarding accountability, by adding dimensions of time and space. More specifically, the study examines the extent to which stakeholders hold corporations accountable after the revelation of an ethical corporate scandal, through a qualitative case study on a pending lawsuit against the Swedish corporation Boliden. Our theory is based on John Roberts' (1991) research presented in the article "The Possibilities of Accountability" and Martin Messner's (2009) research presented in the article "The Limits of Accountability".

This thesis is a cultural theoretical case study of the art project (O)önskad samhällsförbättring.Via an analysis of the project, that is based on theories of power, resistance and contemporaryurban development, it examines the construction of urban space, its impact on public art including the ability of art to initiate evolvement of the urban public space.The last decades art has plaid an important role in what is called the creative city; to become attractive nodal points of the global economy, larger cities increasingly invest in art,culture and creative industry. Simultaneously the urban public space is limited to activitiesand expressions of art that are presumed to create economic growth. This introduces animpression of what is allowed and what is not allowed, possible and not possible, wished andnot wished which, as a consequence, evolves the public space. The events during the artproject and the reactions that followed enlightened these limitations. This tells a story, not only about the structuring process of the local urban space, but also important changes that are created as a simultaneous effect at different levels in society, as well locally as globally.

The paper evaluates two housing proposals with Space syntax as method. The proposals are very different in a topological sense but are in a configural sense almost the same. This effect is because both proposals connect themselves to the existing configural structure of the city in the same way. Neither of them takes advantages of the most integrated streets in the existing structure and use them to concentrate pedestrian and car flows to the streets or points which are intended to be the most urban areas. The knowledge of Space syntax and this evaluation puts focus on the pour understanding among architects and planners of what effect urban design proposals will have..

There exist several known methods for calculation of radio coverage for ground-based systems. As far as we know there are no equivalent methods for the case of flying platforms when the altitudes and speeds are significantly different to those of ground-based systems. This thesis describes the theoretical concepts behind calculations of radio coverage for flying platforms. An investigation is made to sort out what is important and possible to employ in a model for simulations. A method is described and implemented in a program for evaluation of flying radio systems.