Methane (CH4) is a greenhouse gas (GHG) that contributes to the global warming. One of the largest sources of methane is livestock, preferably ruminants which alone counted for 30% of the total agricultural anthropogenic methane emissions in the year of 2000. The reason to why ruminants are such large contributors of methane are that the gas is produced in the rumen by enteric formation and leaves the animals by belching, exhaling or by the excreta.Diets high in concentrates can result in a lower emission of methane. Also diets with a high content of starch, such as alfalfa-grass, have a methane-decreasing. It is profitable to reduce enteric methane formation since that form of methane is unavoidably lost.

This thesis has been performed at Clinical Chemistry at Sahlgrenska University Hospital in Gothenburg. The purpose of the project was to investigate new and alternative ways to determinate synthetic cannabinoids by gas chromatography-mass spectrometry. Currently, the possibilities to quantify synthetic cannabinoids are very limited. This can lead to an increased use of synthetic cannabinoids as the risk of detection is low, which may be known by drug users. The synthetic cannabinoids are sold mixed with different herbs and have varying names like Spice Gold, Spice Silver, K2, Smoke and Pot-pourri.The synthetic cannabinoids analyzed were JWH-018 and JWH-073, which are commonly found in seized Spice material.

With increased milkproduction in our dairy cattle, comes a risk for a reduced fertility. Studies performed on animals and humans have shown that it is possible to manipulate the oestrous cycle through exposure to pheromones. This means that pheromones could potentionally be used to make reproduction more efficient. To evaluate the bioactivity of the substances in question, there is need for a bioassay. In insects, pheromones can induce a changed cardiac activity.

Horses are made to eat mainly roughage and it is essential for optimal health to offer them an appropriate amount of roughage with good hygienic and nutritional qualities. To produce good hay at the right time and be able to keep the hygienic quality until next summer is a problem. This has the effect that horse owners today choose haylage and silage to a larger extent. Science in this area is scarce, i.e. if the change from hay to silage has any effect on the horse.

During the last 50 years the production of milk per cow has increased. This has, however, been followed by a decreased reproductive performance. Shorter and more indistinct signs of oestrus make it harder for the Swedish farmers to discover when the cows are in oestrus. There is a need for ways to control reproduction. The use of pheromones could possibly provide means to satisfy this need.

Bull terrier hereditary nephritis is caused by a mutation that leads to an inadequate synthesis of collagen type IV, which is an important component in the basement membranes. The inheritance of the mutation is autosomal dominant in bull terriers and progression to renal failure takes variable time, from several months to ten years. Proteinuria is the first clinical sign of the disease and the diagnosis is confirmed by transmission electron microscopy of renal tissue where typical ultrastructural changes in the glomerular basement membrane (GBM), thickening and multilaminar splitting are found. This study was performed in order to find out the occurrence of hereditary nephritis in bull terriers in Sweden through examination of urine samples and renal tissue and comparisons with how the disease is described in the literature. Urine samples from 76 Swedish bull terriers were collected and examined for proteinuria.

Acute bacterial prostatitis is a common disease in older male dogs. It gives symptoms such as fever, pain, tiredness, infertility, unwillingness to mate and sometimes symptoms from the urinary tract. It is common that you do an ultrasound exam on dogs when you suspect prostatic changes. In this study 15 dogs diagnosed with acute prostatitis at the small animal clinic at SLU in Uppsala were examined. The clinical signs and the ultrasound pictures were put together in retrospect.

The aim of this study was to evaluate the effects on a simulated wastewater plant regarding GHG emissions and use of energy and resources when implementing source separated wastewater systems. The effects were studied for different restrictions of effluent quality and for different temperatures on the influent. The simulation model BSM2G calibrated for Käppala wastewater treatment plant was used. The task was executed by simulating nine different scenarios with an increase in influent load from new connections equivalent to 3, 10 and 30 % of the present connections. These new connections were served by conventional, urine separated or black water separated systems.

The aim of this study was to investigate the social behaviour and time budget of breedingbulls kept at VikingGenetics, Falkenberg in Sweden when the staff was off duty. It was ofinterest to see if there was any difference between bulls housed in group pens and bullshoused in individual pens. It was also of interest to investigate if there was any differencein the behaviour between the dairy breeds Swedish Red (SR) and Swedish Holstein (SH).Sixteen bulls were used in this study. Eight bulls kept in individual pens and eight bullskept in group pens were used. The individually housed bulls had a social gate with widerbars where the bulls could but their head and neck through to have social contact with thebulls in its neighbouring pens.

In the dog breed Nova Scotia Duck Tolling Retriever (NSDTR) several dogs suffer from joint pain and possibly pain from the muscles. This diffuse disease is called Immune-mediated Non- Erosive Arthritis in Nova Scotia Duck Tolling Retriever. The disease is most likely of autoimmune nature where the etiology still is unknown. The musculoskeletal symptoms of stiffness and shifting lameness are intermittently seen. Some dogs also have dermatological changes and some dogs suffer from pain when opening their mouth. The study included 20 diseased and 20 healthy NSDTR. The purpose was to describe immune-mediated arthritis in NSDTR based on symptoms, results of analysis and effect of treatment. Among the diseased dogs 65% had serum positive for antinuclear antibodies by indirect immunofluorescense (IIF) ANA-test.

I detta arbete presenteras en metod för bestämning av fri kotinin och totala mängden kotinin i urin och serum. Metoden bygger på hydrolys av prov (för total bestämning av kotinin) och extraktion i metylenklorid. Bestämning av kotinin har gjorts med HILIC-MS/MS..

The most common way to treat wastewater in Sweden today is by a combination of an activated sludge process and a chemical treatment. These processes are not very energy efficient and may not be the most environmentally friendly. That is why it is interesting to evaluate new alternative methods to see if they can be less harmful to the environment and help to a lower resource consumption. New techniques are tested in a pilot installation at Hammarby Sjöstad, Sjöstadsverket. To be able to evaluate these techniques in a wide perspective system analyses are conducted where different impacts can be considered, advantages and disadvantages in the systems.Five system structures for water treatment have been constructed in Matlab/Simulink in this study.

AbstractThis study deals with preschool children's ideas about water in our body. Ten children with the age of four to five were interviewed individually, the interviews were qualitative. The children were also asked to draw their answers and answer questions about a picture that represented the excretory system. The results of my study show that children did not have any knowledge about the water being vital for us. Preschool children had no knowledge of why it is important to drink water.

There are around 855 000 on-site sewage systems in Sweden and some 1 800 of these are located in the municipality of Sigtuna. The Sigtuna local authority has set the goal that all sewage systems with insufficient function should be improved before the end of year 2010. A malfunctioning on-site sewage system may cause three main problems: spreading of diseases, discharge of eutrophicating compounds and wastage of resources. The municipality strives to reduce these problems already at the stage of granting permits for installation of on site sanitation systems. The municipality has recently adopted new guidelines, which demands certain minimum reductions for different compounds. The guidelines also state that it is preferred that neighboring house-owners cooperate in jointly built and operated sewage systems and that the system should be able to recycle plant nutrients.

Ambulatory surgery is common and develops alongside surgery and anesthetic methods. Time admitted is short and aftercare takes place in the home environment.Objective: The aim of the study was to investigate which symptoms patients experience after ambulatory surgery related to anesthesia. Furthermore how strongly symptoms affect the patient. Symptoms appear as a result of anesthesia and surgery and can therefore be interpreted as nursing induced suffering.Method: Participants were recruited at ambulatory clinics at a Swedish university hospital spring, 2015.  Prior to the study permission was granted from the clinic managers. The study is a quantitative descriptive longitudinal study.  Data collection forms were administered and data collection was performed by phone.