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Antibiotic resistance associated with bacteria in irrigation water : a case study of irrigation ponds in Southern Sweden
The
focus
of
this
project
was
to
characterize
the
occurrence
of
ESBL-?producing
bacteria
in
two
irrigation
water
ponds
in
Southern
Sweden.
Samples
were
taken
from
two
ponds
nearby
each
other,
from
which
analyses
were
made
based
on
levels
of
community
and
individual
isolates.
Community
samples
were
used
for
attempts
for
characterization
of
resistance
patterns
in
irrigation
water
using
the
Omnilog
PM-?plate
system.
The
growth
of
community
microbiota
in
the
presence
of
the
following
antibiotic
substances
was
assessed;
amikacin,
gentamicin,
kanamycin,
penicillin
G,
ampicillin,
piperacillin,
trimethoprim,
potassium
tellurite
and
ciprofloxacin.
Isolates
were
pure
cultured
using
semi-?selective
media:
LB,
Rainbow
Agar,
mEnterococcus,
0.1
TSA
and
VRBD.
A
total
of
177
isolates
were
isolated
randomly.
Each
isolate
was
identified
using
the
Omnilog
GENIII-?system
of
biochemical
characterization.
Further,
the
isolates
were
tested
on
Brilliance
ESBL-?agar
for
their
ability
to
grow.
Out
of
the
total
number
of
isolates,
30
were
selected,
preferably
Enterobacteriaceae
or
Pseudomonas
spp.,
based
on
their
high
similarity
to
library
strain
during
the
identification
in
the
Omnilog-?system.
These
were
further
characterized
using
Etest-?sticks.
Three
selected
strains
were
picked
for
further
analyses
using
exogenous
isolation
for
attempts
to
simulate
horizontal
gene
transfer,
and
Omnilog
PM-?panels
containing
various
antibiotics
of
interest.
Selections
of
samples
were
chosen
for
molecular
tests
targeting
plasmid-?borne
resistance
genes
CTX-?M1,
CTX-?M2
and
SHV.
The
method
for
assessing
community-?resistance
patterns
showed
ambiguous
data
that
was
difficult
to
interpret;
further
optimization
is
recommended.
For
all
tested
PM-?plates,
generally
greatest
differences
between
substances
were
found
among
wells
with
the
strongest
concentration
of
antibiotic
substance.
A
total
of
175
isolates
displayed
ability
to
grow
on
ESBL-?agar.
Etests
confirmed
ability
of
isolates
to
resist
many
?-?lactam
antibiotics
and
many
were
clinically
resistant
to
certain
substances.
No
plasmids
were
transferred
during
exogenous
isolation.
During
molecular
tests,
a
few
matches
were
found
for
the
CTX-?M1
gene.
Isolates
analyzed
with
Omnilog
PM-?plates
showed
resistance
to
most
compounds
tested.
The
isolates
showed
greatest
resistance
toward
penicillin
G,
ampicillin,
amikacin,
kanamycin
and
trimethoprim.
In
the
study,
it
was
established
that
resistance
among
bacteria
was
frequently
occurring
in
the
irrigation
water,
and
?-?lactamase
producing
bacteria
was
very
common.
Extended
studies
are
needed
to
assess
the
frequency-?
and
occurrence
of
horizontal
gene
transfer
(HGF)
in
this
environment.
Although
some
potential
corresponding
data
is
found,
it
is
at
this
point
not
possible
to
determine
whether
the
resistances
are
due
to
antibiotics
used
in
veterinary
medicine,
human
medicine
or
one
additional
reason
is
travel
and
trade.
in
irrigation
water
using
the
Omnilog
PM-?plate
system.
The
growth
of
community
microbiota
in
the
presence
of
the
following
antibiotic
substances
was
assessed;
amikacin,
gentamicin,
kanamycin,
penicillin
G,
ampicillin,
piperacillin,
trimethoprim,
potassium
tellurite
and
ciprofloxacin.
Isolates
were
pure
cultured
using
semi-?selective
media:
LB,
Rainbow
Agar,
mEnterococcus,
0.1
TSA
and
VRBD.
A
total
of
177
isolates
were
isolated
randomly.
Each
isolate
was
identified
using
the
Omnilog
GENIII-?system
of
biochemical
characterization.
Further,
the
isolates
were
tested
on
Brilliance
ESBL-?agar
for
their
ability
to
grow.
Out
of
the
total
number
of
isolates,
30
were
selected,
preferably
Enterobacteriaceae
or
Pseudomonas
spp.,
based
on
their
high
similarity
to
library
strain
during
the
identification
in
the
Omnilog-?system.
These
were
further
characterized
using
Etest-?sticks.
Three
selected
strains
were
picked
for
further
analyses
using
exogenous
isolation
for
attempts
to
simulate
horizontal
gene
transfer,
and
Omnilog
PM-?panels
containing
various
antibiotics
of
interest.
Selections
of
samples
were
chosen
for
molecular
tests
targeting
plasmid-?borne
resistance
genes
CTX-?M1,
CTX-?M2
and
SHV.
The
method
for
assessing
community-?resistance
patterns
showed
ambiguous
data
that
was
difficult
to
interpret;
further
optimization
is
recommended.
For
all
tested
PM-?plates,
generally
greatest
differences
between
substances
were
found
among
wells
with
the
strongest
concentration
of
antibiotic
substance.
A
total
of
175
isolates
displayed
ability
to
grow
on
ESBL-?agar.
Etests
confirmed
ability
of
isolates
to
resist
many
?-?lactam
antibiotics
and
many
were
clinically
resistant
to
certain
substances.
No
plasmids
were
transferred
during
exogenous
isolation.
During
molecular
tests,
a
few
matches
were
found
for
the
CTX-?M1
gene.
Isolates
analyzed
with
Omnilog
PM-?plates
showed
resistance
to
most
compounds
tested.
The
isolates
showed
greatest
resistance
toward
penicillin
G,
ampicillin,
amikacin,
kanamycin
and
trimethoprim.
In
the
study,
it
was
established
that
resistance
among
bacteria
was
frequently
occurring
in
the
irrigation
water,
and
?-?lactamase
producing
bacteria
was
very
common.
Extended
studies
are
needed
to
assess
the
frequency-?
and
occurrence
of
horizontal
gene
transfer
(HGF)
in
this
environment.
Although
some
potential
corresponding
data
is
found,
it
is
at
this
point
not
possible
to
determine
whether
the
resistances
are
due
to
antibiotics
used
in
veterinary
medicine,
human
medicine
or
one
additional
reason
is
travel
and
trade..
En väg från utanförskap till riktiga jobb : Karriärstödets betydelse för deltagare i sociala arbetskooperativ
Arbetsmarknaden är en viktig arena för att människor ska känna sig delaktiga i samhället. För dem som länge befunnit sig i ett utanförskap är det svårt att få ett jobb. Sociala arbetskooperativ har som mål att integrera utsatta grupper i arbetslivet och har blivit ett viktigt inslag för en inkluderande arbetsmarknad. Undersökningen omfattar tre sociala arbetskooperativ som ingår i ett konsortium. Arbetssättet som används i den arbetslivsinriktade rehabiliteringen kallas karriärstöd.
The black-and-white colobus monkeys (Colobus angolensis palliatus) of Diani forest, Kenya : behavioural responses to habitat fragmentation
The loss of habitat is one of the greatest threats to global biodiversity today. Consequences to a species inhabiting fragmented habitats include: group size changes, altered diets and altered foraging behaviours.
In my study I observed Colobus anlgolensis palliatus troops living in the forests of Diani, a highly developed area on the Kenyan coast. I looked for differences in behaviours between three troops inhabiting pristine forest patches and three troops inhabiting highly degraded forest areas. I used focal animal sampling with instantaneous sample points.
"Hur fan kan man jobba som kriminalvårdare?": en studie i hur kriminalvårdare ser sin yrkesroll ur en psykosocial kontext
This analysis explores the psychosocial aspects in relationship to the occupational role as a correctional officer in Sweden. Historically prisons are hierarchically structured with a military organisation used as the basis for a role model. This military type structured organisation is designed to keep the prisoners under constant observation, thus providing direct authority and control by the prison guards over the prison inmates. Data obtained from investigations conducted by the Swedish Government indicate a direct link between the prisoners's living conditions and the prison employee's work duties and environment. The data strongly suggests that the work needs of prison guards have significant priority over the general work conditions of inmates, thus showing that the status for the occupational role was relatively low.
?Det verkar som man helst vill vara någon som är blond, smal och glad? : En komparativ studie av två olika chefredaktörers versioner av Veckorevyn
The purpose of this study is to compare the contents, editorials and front covers of Sweden?s oldest girl?s magazine Veckorevyn during two different years, in which the magazine had two very different chief editors.In the year 2000, Emma Hamberg got the job as editor-in-chief for Veckorevyn, with a vision of changing the way the magazine represented young females. She wanted to add a more feminist viewpoint to the magazine, since she felt that Veckorevyn didn?t live up to its claim of being ?a girl?s best friend?. Six years later, the famous Swedish fashion editor Ebba von Sydow became editor-in-chief for Veckorevyn, with the intention of making the magazine better, smarter and more fun for young girls.
Samvetsfrihet : En studie om vårdpersonals rättigheter till och upplevelser av samvetsfrihet vid abortverksamhet i Sverige och Norge
The right to freedom of conscience is protected by international conventions and declarations of human rights that have been ratified by Sweden. Nationally, the issue of health professionals? right to freedom of conscience in the abortion care is not resolved by public inquiry. This has led to that Sweden has been notified to the European Committee of Social Rights on the grounds that the country is considered to be violating the Council of Europe Resolution 1763 and that the proper question to be determined by regulations of the interests that are balanced, based on their legal value in relation to each other. Freedoms, rights and obligations conflict with each other.
Ahmed, Adam och de asatroende : En undersökning av två samtida skildringar av offer i den fornnordiska religionen
This essay takes its start in the problematic situation concerning source material in the study of the Norse religion before the Christianization of Scandinavia. There is a lack of written sources from the time when the religion was still practiced. There are plenty of archeological sources economic situation than their religious beliefs. The Icelandic stories written in the 13th and 14th centuries give us a broad pictureof the Norse mythology, but the writers were Christians which makes their reliability questionable.The focus of this essay is therefore on two texts written during the time when the Norse religion was still in practice. The first source is the travel notes written in 922 by the Muslim scholar Ahmad ibn Fadlan who met a group of the Rus? people.
Vägen till toppen : En studie om kvinnliga VD:ar och deras karriärer
Trots att allt fler kvinnor tar plats i näringslivet så domineras såväl näringslivet som de flesta chefspositioner fortfarande av män. Ständigt pågår diskussioner om vad det är som skapar denna sneda fördelning, samt hur kvinnor är i en avvikande position på grund av sin minoritet och huruvida det finns hinder som gör det svårt för kvinnor att nå upp till toppen. Oavsett hur detta stämmer eller ej är det endast individuella upplevelser av kvinnor som lyckats ta sig till toppen som närmast kan förklara hur verkligheten ser ut.Med detta som utgångspunkt ämnar studien undersöka kvinnliga VD:ar inom det svenska näringslivet för att försöka ta reda på, samt redogöra för, hur de upplever att olika möjligheter och eventuella hinder har spelat roll för deras respektive karriärer. Studien är av kvalitativ utformning, där data samlats in genom semistrukturerade intervjuer. Det sammanställda resultatet kommer därefter att analyseras ställt mot valda teorier och modeller, för att slutligen diskuteras med inslag av egna reflektioner och slutsatser.Studien visar på hur majoriteten av kvinnorna stött på fler möjligheter än hinder i sin karriär, trots att de suttit i avvikande positioner som kvinnor i mansdominerade branscher.
Kvinnliga ledare inom banksektorn
Title: Female leaders within the bank sector. Level: D-level in Business Author: Emma Roos & Fanny Wiklander Supervisor: Maria Fregidou-Malama Date: 2011 ? January Aim: The aim of this study is to examine which factors are behind the low number of female leaders compared to male leaders within the bank sector. Method: We have adapted a qualitative method. This means that the researcher works close to the object of research and tries to understand it. We believe that this is a suitable method for our study since it?s based on seven personal interviews.
Destinationsutveckling under politisk kris : En fallstudie om Tunisien 2011
The authors have during the spring semester 2011 studied the subject destination development during a political crisis, focusing on Tunisia. At the beginning of 2011 the country was in an uncertain political situation when the revolution of the Tunisian people occurred. This created massive demonstrations on the streets, and resulted in the current government's resignation. More effects of the revolution has been seen in a drastic decline of inbound tourists in the country, which in turn affected the country's population and economy. From this point on Tunisia as a destination needs to recover to once again become attractive to tourists.
Vem tänder stjärnorna? : Gotländska studie- och yrkesvägledares arbete med genus-, klass- och etnicitetsfrågor
Syftet med vår uppsats är att undersöka om de gotländska studie- och yrkesvägledarna arbetar med genus-, klass- och etnicitetsfrågor. Vi vill ta reda på vilka metoder som används och om något samarbete sker runt dessa frågor. Utöver detta är vi intresserade av att ta reda på om verksamhetscheferna tar ansvar för detta uppdrag och formulerar hur man har tänkt sig att arbetet skall gå till. Intresset har väckts under vår tid på studie- och vägledarutbildningen i Stockholm och främst som ett resultat av vår B-uppsats, då vi granskade vilken kunskap rektorerna hade om studie- och yrkesvägledarnas kompetens och i vilken mån studie- och yrkesvägledning hade tagits upp i rektorsutbildningen. Vi fann att rektorerna hade mycket liten kunskap om vägledning och att rektorsutbildningen inte hade berört detta område alls.
Don't mention the "L"-word. En analys av biblioteks- och informationsvetenskapsutbildades karriärmöjligheter på den alternativa arbetsmarknaden.
This master's thesis is about alternative career opportunities for library and information science graduates. Ever since the beginning of our education we have been interested in working outside of the traditional libraries. Now that we are about to receive our degrees in library and information science we are doubtful if the competence we have obtained is enough to meet the demands of the labour market. The employment field we have been studying in this master's thesis is information management, since this is a field that we find comparable to library and information science. Our study involves a qualitative survey amongst recruiting companies to investigate how the recruiters value different educations, competences and personal skills when they employ staff within the field of information management.
Om Lettlands utveckling som destination : en jämförelse av turismindustrin under landets självständighetsperioder
Latvia has been an independent state twice, the first time during the years 1918-1940 and the second time from the year 1991 and ahead. During these two periods Latvia has been a destination for tourists. The aim of this study is to describe how Latvia as a destination has developed during the years of independence. One further aim is to compare what Latvia?s tourism industry looked like during the same periods to descry similarities and differences.
Studie- och yrkesvägledares yrkesidentiteter : En intervjustudie med några verksamma studie- och yrkesvägledare på grund- och gymnasieskolan
AbstractFörfattare: Liselott HolstTitel: Studie- och yrkesvägledares yrkesidentiteter- En intervjustudie med några verksamma studie- och yrkesvägledare på grund- och gymnasieskolanMasteruppsats: 91 ? 120 hpHandledare: Niklas GustafsonPedagogiska Institutionen Bakgrunden till denna uppsats är att det riktats en hel del kritik mot skolans studie- och yrkesvägledning av bl.a. Skolverket. Ur dessa rapporter framkom bl.a. kritik mot att det idag finns stora skillnader i kvalitet mellan olika kommuner och olika skolor när det gäller skolornas studie- och yrkesvägledning.
A competitor?s reflections : reflections about competing in an international architectural competition: LE:NOTRE Student Competition - (re)discovering the emerald necklace of Colentina
In the profession of architects there is an element called architectural competitions,
where several architects compete in submitting the best solution for a certain task. In
this master?s thesis I am studying the competitor?s perspective of an international
architectural competition, while at the same time gaining experiences for my future
career as a landscape architect. The aim of this thesis is to through an introspection of
my own process examine a competitors view of the international architectural
competition and how to approach the difficulties concerning international competitions.
Through that I hope obtain experience about the international competition as part of
the profession of landscape architects and other involved professions. To examine a
competitor?s reflection of an international competition, I entered and competed in
LE:NOTRE Student Competition, a competition for students where the task was to
develop 25 kilometers of complex urban periphery along a chain of natural and artificial
lakes in Bucharest, Romania.