Syftet med denna litteraturstudie är att genom granskning av vetenskaplig litteratur undersöka hur de riskfaktorer i arbetet som orsakar utbrändhet bland sjuksköterskor kan förebyggas. Frekvensen av utbrändhet i Sverige har ökat sedan slutet av 90-talet och de riskfaktorer som har identifierats av olika forskare är av organisatorisk, individuell och social genes. För att uppnå syftet gjordes en litteraturstudie som grundar sig på åtta vetenskapliga artiklar. Som teoretisk referensram valdes Demerouti´s Job Demand-Resourcesmodell för att tydliggöra begreppet utbrändhet. Mätinstrumentet MBI användes också för att förklara hur utbrändhet kan mätas.

Anthelmintic resistance is a major problem in sheep husbandry all over the world. One way toslow down the development of anthelmintic resistance is to keep part of the parasitepopulation in refugia (unexposed to drugs) which will maintain the genes for susceptibilitywithin the population. Climate, type of parasite and drenching regimes effect the size of therefugia. Dilution of resistant with susceptible parasites, targeted treatment and targetedselective treatment are all management strategies that employ refugia. Dilution is possible butcomplicated.

Urinvägsinfektioner orsakar ett stort lidande då infektionen är smärtsam och ofta återkommande. Lågdosantibiotika som idag används i preventivt syfte vid återkommande urinvägsinfektion bidrar till resistensutveckling, förlängda vårdtider och ökade sjukvårdskostnader. Syftet med denna litteraturstudie är att undersöka tranbärets effekt som prevention vid urinvägsinfektion, för att eventuellt kunna rekommendera tranbär som alternativt profylax. Som metod valdes litteraturstudie. Resultaten visar att tranbär har en preventiv effekt, som verkar även på antibiotikaresistenta bakterier.

Totally 84 differentially expressed mRNA clones from infective L3 larvae of the parasite Haemonchus contortus, a blood sucking nematode, were analyzed with single strand hybridization assay (SSH). Altogether 79 clones were sequenced, edited, and compared with proteins found via BLAST in GeneBank. The aim was to investigate gene expression and potential protein expression following storage at 5 °C for 32 weeks. mRNA was extracted from fresh and stored L3. The SSH derived products were cloned into E.

The objectives of this study was to examine the prevalence of hypertrophic cardiomyopathy in a family of British Shorthaircats to determine the mode of inheritance and to investigate if the disease was associated with a mutation in myosin binding proteinC (MyBPC3). The family comprised 28 cats, seven male and 21 females. The cats underwent a physical examination, including cardiac auscultation, and an ultrasound examination. Blood was collected for DNA- analysis. The blodsamples were sent in a buffer solution to Kathryn Meurs, Washington State University, USA for analysis, where the genes coding for MyBPC, Troponin I and T were characterized using microsequencing technique. Out of the 28 cats, 8 were diagnosed with HCM, 2 were diagnosed with congenital heart disease but had no evidence of HCM.

Toxic metals contaminate soil worldwide and thus serve as sever environmental threat. Therefore the purposes of this study were to investigate in which soil fractions that different heavy metals (Fe, As, Cu, Zn and Pb) could be found in contaminated soils and if it is possible to use sieving as a method for decontamination. Soil samples were collected from three different locations, the Nasa silver mine, the Blaiken-mine and Svalget environmental station. The samples were oven dried and later on sieved into six different fractions 8mm, 4 mm, 0.5 mm, 0.250mm, 0.063 mm and <0.063 mm. The fractions 4 mm, 0.5 mm and <0.063 mm from each location were analyzed in an x-ray fluorescence detector.

Stormwater is surface runoff water that originates from precipitation or snowmelt and that on hardened surfaces prevents to infiltrate the ground. In city areas stormwater can consists of major amounts pollutants and when reaching a lake or watercourse it can cause damages on vegetation and animals. Therefore it is important to treat polluted stormwater before it reaches the recipient. The municipality of Örebro have since the 90ies an extensive work with treatment of stormwater in sedimentation ponds. A new stormwater treatment project is planned in an industrial estate in Skebäck, Örebro.

During the last few years the presence of high arsenic (As) concentrations in ground water has been of major concern both internationally and in Sweden. Much evidence has been reported about toxic effects caused by arsenic. The carcinogenic effects and the possibility to measure the toxic impacts at low concentrations made the World Health Organisation (WHO) to reduce the guideline value from 50 to 10 ?g l-1 in 1993. In Sweden the corresponding reduction was implemented in 2003. Several surveys in Sweden have shown that high arsenic concentrations may occur in ground water.

Genetic defects are caused by mutations in major genes where the gene?s protein product has a large impact on the physiology of the animal. The synthesis of the protein can be altered by a change in the nucleotide sequence, which can lead to malformation and in many cases death.One of the main reasons of increase in many genetic defects is the use of few bulls in breeding programmes, causing a reduction of the genetic variation. Genetic defects cause suffering for the animal and influences the production by, for example, increased costs due to misscarriages, lost milk production and expenditure for medical treatment. Bovine Leukocyte Adhesion Deficiency (BLAD) and Complex Vertebral Malformation (CVM) are two genetic defects that were widespread before the causative mutation was discovered.

The use of intramuscular injections of procaine penicillin G (bensylpenicillin procaine) in the horse is now and then associated with acute adverse reactions (penicillin-chock). The etiology is not yet clearly understood, but the theories are several. One possibility is that it can be caused by procaine toxicity. Procaine penicillin G is a salt which is quickly dissolved in plasma. Procaine is then metabolized by plasmaesterases to non-toxic metabolites, p-aminobenzoic acid (PABA) and diethylaminoethanol.

During the 1960´s and 1970´s the number of grey seals in the Baltic Sea was decreasing rapidly, mostly due to hunting and toxic substances like DDT and PCB. When hunting became less intense and toxic substances decreased in the environment the grey seal population started to increase. Today grey seals are found common in the Baltic Sea and have started to become a big treat and a problem to the fishing industry. The grey seal destroys and enters fishing traps and consumes large quantities of the fish that have been caught.The knowledge of the grey seal, like abundance and food preferences, is today limited. It is also important to define the position of the grey seal in the ecosystem in the Baltic Sea and to be able to predict changes that could occur if the population would rapidly decrease or increase.

During the 1960´s and 1970´s the number of grey seals in the Baltic Sea was decreasing rapidly, mostly due to hunting and toxic substances like DDT and PCB. When hunting became less intense and toxic substances decreased in the environment the grey seal population started to increase. Today grey seals are found common in the Baltic Sea and have started to become a big treat and a problem to the fishing industry. The grey seal destroys and enters fishing traps and consumes large quantities of the fish that have been caught.The knowledge of the grey seal, like abundance and food preferences, is today limited. It is also important to define the position of the grey seal in the ecosystem in the Baltic Sea and to be able to predict changes that could occur if the population would rapidly decrease or increase.

Rhodesian ridgeback is a dog breed that originates from southern Africa. The characteristic ridge (a dorsal ridge where the hair grows in the opposite direction to the general coat) is shared with an Asian breed, Thai ridgeback. The origin and inheritance of the ridge has been examined and defined. The ridge-mutation is a duplication that contains four complete genes, FGF3, FGF4, FGF19 and ORAOV1 and the 3´-end of CCND1. The ridge is inherited as an autosomal, dominant trait and predisposes for Dermoid sinus (DS), a disease that develops during embryogenesis.

Rheumatoid arthritis is an autoimmune disease, where joints are attacked by the own immune system, leading to chronic inflammation and destruction of bone and cartilage. Inflammation is a complex process, controlled by many different substances. One of them is adenosine, which has anti-inflammatory properties. In this project, three polymorphisms in different genes, involved in synthesis and signaling of adenosine, were genotyped for 188 patients with RA and 362 controls without RA. The results shows that for the polymorphism in A2a, a gene coding for an adenosine receptor, there was no significant difference in genotype distribution between the groups.

Ideonella dechloratans är en av flera isolerade bakteriestammar med förmågan att använda klorat i sin metabolism i anaerob miljö. Detta kan utnyttjas inom exempelvis pappersindustrin där klorat är en miljöfarlig restprodukt efter klordioxidblekning.   Klorat och perklorat har visat sig ha negativa effekter hos både människor, djur och växter vilket ger ett stort behov av mer forskning på förbättrade reningsmetoder. Kan genregleringen av enzymerna som sköter nedbrytningen av klorat, kloratreduktas och kloritdismutas, förbättras genom att exempelvis fungera även i aerob miljö så skulle reningen förbättras samt kostnader dras ner.   Genom att införa promotorregionerna för kloratreduktas och kloritdismutas i en broad-host-range reporterplasmid, pQlacZ-1, så kan dessa testas i flera olika förhållanden och i olika typer av gramnegativa bakterier.   Genom dubbelklyvning av pQlacZ-1 med BamHI och EcoRI så har PCR produkter av Clrp och Cldp från Ideonella dechloratans kunnat ligeras in för att sedan transformeras in i E. coli, XL-1 Blue.    Gelextraktion har visat sig vara den effektivaste reningsmetoden inför ligering men utförligare screening behöver göras på transformanterna för att säkerställa metodens effektivitet..