Vectors based on lentivirus backbones have revolutionized our ability to transfer genesinto many cell types. Lentiviral vectors integrate into the chromatin of target cells and do not transfer any viral genes causing vector replication. Both of these features arecommonly used in gene therapy and have been used clinically in individuals sufferingfrom cancer, infections and genetic diseases. It has been discovered that T-cells can be genetically modified to be used as effective weapons against cancer: therefore virus mustbe produced to deliver the gene of interest into the T-cells. In this project, lentiviralvectors have been produced to transfer the gene coding for a chimeric antigen receptor(CAR) which is directed to CD19 on B-cells.

Background: In today's Swedish health care, it is required that the various professionals must work together in teams to safer care. Several professionals with various skills expected working together in the traumateam at the care of the traumapatient. At the same time, research shows that substandard teamwork leads to avoidable errors in care. One way to improve teamwork is to practice. Purpose: The purpose of this study is to shed light on the experiences of the traumateam exercises that exercise participants feel has been favorable for the patient at the initial care of trauma patients.

The aim with this thesis is to investigate two different methods used to test usability on web-sites to determine the advantages and disadvantages of two methods and compare the results. The two selected methods are the think aloud method and asynchronous remote method. Re-mote usability testing has existed for more than ten years but the possibilities with this meth-od needs to be explored more.To achieve the purpose of this thesis a case study was conducted on an e-commerce site and for the online remote usability testing software was used.The results of this study show that think aloud usability testing encounter more issues with the website than the remote usability testing..

The Pitted ware dwelling site Ajvide on the west coast of Gotland in the Baltic Sea is, because of its long continuity from the late Mesolithic to the middle of the Scandinavian Bronze Age, an interesting research object concerning Stone Age in the Baltic area. In this essay, a comparison between two activity areas on Ajvide have been carried out in the purpose of finding out what these areas may have been used for during the middle Neolithic.  It has been showed, through zooarchaeological analyses of animal bones, that the areas most likely are depositing sites for slaughters refuse and food debris. If it is a result of everyday or more ceremonial activities is left undetermined..

AbstractThe research project Liberation, supported by the European Commission under the Environment and Sustainable Development Programme (Fifth RTD Framework Programme) aimed at developing a decision support system for ecological risk assessment of contaminated sites has proposed a tiered system including the triad method. This method integrates contaminant chemistry and bioavailability analyses with observed ecotoxicological effects in soil and groundwater. In this study the triad method was used to perform a risk characterization of a former surface treatment and metal industry, now used for the student union buildings of the Mälardalen University in Eskilstuna, Sweden. On the site, high concentrations of copper, zinc and nickel have been detected in the soil in a preliminary investigation of contaminant levels with XRF. For performing the risk assessment of the site according to the triad method, contaminant bioavailability, soil metal concentrations and soil toxicity were determined and an ecological assessment was carried out on four selected soil samples (one reference soil and three samples with low (B), medium (C) and high (D) contaminant levels). Contaminant bioavailability was measured through leaching (ISO TC 190/S7) and biouptake tests (DGT).

Elective joint surgery increases for every year and the operations are associated with postoperative pain. Postoperative pain is a well known phenomenon and pain physiology and analgesics are well known by caregivers, but the significance of the interaction between patient and caregiver is not illuminated. There are still patients experiencing unnecessary postoperative pain. Among patients in elective surgery, 25% runs a risk for underestimated pain. The aim of the study was to describe patient?s experiences of nursing interaction in the context of postoperative pain during hospitalisation after going through knee- or hiparthropasty.

In this paper I will attempt to recreate a prehistoric landscape surrounding the rock carving site of Släbro at Nyköping in Södermanlands län by using GIS. The main focus area is the river valley at Släbro and the six panels with rock carvings that have been dated to the Bronze Age. I?ll attempt to shed light on a long lost landscape. The shore line displacement have been extensive in this area a reconstruction will visualize the relationship between the panels, and their relationship to the surrounding landscape.

Population growth leads to an increased need for new buildings, both residential and commercial. The regulations of structural design also changes with time. From a sustainable community perspective it may be profitable if existing buildings, that no longer performs their original function, are reconditioned. Knowledge and understanding of the surroundings of the residence must be available. Regulations regarding accessibility to all has increased.

Elective joint surgery increases for every year and the operations are associated with postoperative pain. Postoperative pain is a well known phenomenon and pain physiology and analgesics are well known by caregivers, but the significance of the interaction between patient and caregiver is not illuminated. There are still patients experiencing unnecessary postoperative pain. Among patients in elective surgery, 25% runs a risk for underestimated pain. The aim of the study was to describe patient?s experiences of nursing interaction in the context of postoperative pain during hospitalisation after going through knee- or hiparthropasty.

Fresh Kills park and the The High line park in New York, Downsview park outside Toronto the birch landscape at Shiphol Airport have something more in common than being big new park landscapes.They all include thoughts and images about ecology. These images are neither typically nature picturesque nor typically garden stylish. In Sweden we have the biotopes of Ankarparken, the new design of Kristinebergsparken. At Chelsea Flower Show in England Swedish nature put in a garden format is winning awards. There are some new ecological influence in the air.

This report presents the preliminary study and work for the development of a site structure for the upcoming reengineering of inter-tool Infopunkt. The report describes how I have conducted a study regarding the current inter-tool information center and its development potential. Thereafter compiling the information to develop a comprehensive design approach to create a sitemap. The sitemap illustrates a new updated page structure for Infopunkt and was the primary goal of the thesis. The sitemap I have developed will form the basis for the agency that will later on develop the new updated version of Infopunkt..

In this thesis we propose a conceptual plan for thedevelopment of an Agroforestry Training Centre (ATC)in Musoma, Tanzania. The aim is to make a proposalto support peasant education in agroforestry. How canan ATC in Musoma be developed into a stimulatingand educational park suitable for teaching agroforestrymethods to peasant farmers and other potential users?To answer that a field study was conducted to investigatehow our client Vi Agroforestry Programme (Vi) and thetarget groups of the client can benefit from the site.The thesis begins with a short explanation of thecontext, in which the ATC plays a part followed by amethodology chapter. Next are three chapters presentedwhich introduce the reader to the research conductedbefore starting the proposal, these include: results ofliterature studies, study of precedents, and results of fieldstudy.

Rabbits carry a high risk of anesthesia related death. This study was part of a project to develop a form of total intravenous anesthesia (TIVA) with minimal effects on cardiac, respiratory and metabolic parameters. In the study, TIVA with sufentanil and midazolam (group TIVA, n=9) was compared with inhalation anesthesia with sevoflurane (group SEVO, n=9) in female rabbits undergoing surgery (ovariehysterectomi). All rabbits were pre-medicated with medetomidin and carprofen before anesthesia induction. Anesthesia was induced with TIVA (2.3 ?g/ ml sufentanil, 0.45 mg/ ml midazolam) at a rate of 4 ml/ kg/ h and the mean induction dose was 0.4 ?g/ kg of sufentanil and 0.1mg/ kg of midazolam.

In today?s technological society, there are increasingly less physical demands on the locomotor system. However, despite that notion strength and function in the hand?s complex biomechanical system are in fact both needed to cope with daily life. The grip function is a main function in the hand and is used in many examples of scientific research, for instance when assessing physical health and predicting the probabilities of premature mortality.

Syfte: Att beskriva dagkirurgiska patienters upplevelse av att bli utskrivna hem samma dag efter operation utförd i generell anestesi. Metod: Kvalitativ intervjustudie med induktiv ansats. Elva patienter som har opererats via en handkirurgisk mottagning i mellersta Sverige har intervjuats utifrån en semistrukturerad intervjuguide. Sju kvinnor och fyra män i åldrarna mellan 28-71 år har deltagit i studien. Innehållsanalys användes för analys av materialet.