Maize for silage has been used as fodder in different parts of the world for a long time and has recently become more common also in Sweden. Our cold climate has been a problem as maize is very sensitive to low temperatures and frost. With new varieties and better techniques for cultivation it is now possible to grow maize in the southern and middle parts of Sweden. As for the north of Sweden the length of the cultivation season is a limiting factor as there are too few days with temperatures of over +10 ºC. Maize is well suited for silage making but oxygen-free conditions are required.

The use of forage maize has increased over the last years. The aim of this litterateur review was to summarize a part of the research that has been conducted on forage maize for growing cattle, including cultivation and conservation of the maize. The forage maize is planted in April or the beginning of May when the temperature in the soil is between 6 and 10°C. Maize needs high temperatures and much water. Maize is harvested in the autumn when the dry matter (DM) is more then 30% or the plants have been exposed to the first frost in the autumn.

Kolorektal cancer (CRC) är en av de vanligaste cancersjukdomarna i världen med cirka 1 miljon nya detekterade fall per år. Special AT-rich sequence-binding protein1 (SATB1) är ett celltyp-specifikt kärnmatrix-associerat DNA-bindande protein, vilket utgörs av AT-rika DNA sekvenser. Det har tidigare demonstrerats att en annan medlem i SATB-familjen, SATB2, uttrycks på ett vävnadsspecifikt sätt i normal mukosa i nedre mag-tarmkanalen och i CRC. ?-catenin är en intracellulär mediator i Wnt/?-catenin signaleringsvägen, som spelar en viktig roll i kolorektal carcinogenes.

Horse owner?s knowledge about horse feeding and nutrition is directly linked to the horse?s health and welfare. According to several studies, both Swedish and foreign, horse owners generally have inadequate knowledge about horse feeding. The housing systems and feeding routines of today differs in many ways from what horses have adapted to through evolution. Wild horses live in harem herds and roam on large open fields, spending about 60 % of the day grazing, mainly grass and herbs.

Den senast utvecklade tekniken för riktade förändringar av genom in-vivo är baserad på CRISPR/Cas-systemet för vilket förutsättningarna i Nicotiana benthamiana och potatis, Solanum tuberosum, här undersöktes. För att föra in genkonstruktioner i växterna användes Agrobacterium tumefaciens som via vakuum kommer i kontakt med de inre delarna av bladen varefter de gener som finns på en binär vektor uttrycks transient. Först undersöktes de optimala parametrarna för erhållandet av ett högt transient uttryck med hjälp av ett grönt fluorescerande protein, GFP. Dessa parametrar användes sedan för att infiltrera en CRISPR/Cas-konstruktion som designats för att introducera mutationer genom att klyva ett exon i genen för granulbundet stärkelsesyntas, GBSS. Det visade sig under första delen av arbetet att det ej gick att erhålla transient uttryck i potatis med denna metod.

Background: There have been doping usage as far back as as ancient Greece and around the early 1900s started the doping we have today. In 2008 it was 22,000 men aged 15-54 years old who had any kind of experience with doping in Sweden. The problem of doping use are all over the world and to overcome this problem, we need to work across borders.Objectives: The purpose of this study is to investigate whether there is any connection between the use of supplements and attitude to doping. The study is based on the questions: is there any connection between the use of supplements and positive attitude to doping? is there any connection between the use of supplements/doping substances and high training frequency? and is there different use of supplement/doping substances between girls and boys?Method: A survey in form of a cross-sectional study and a waiting room survey was conducted with 63 respondents of whom 57,1% were women.

Milk fat is an important feature in many different milk products and other foodstuffs and it is often crucial for the dairy plants that the milk fat is stable for different manufacturing processes. Lipolysis is the enzymatic degradation of fat and is the one of the causes for an elevated amount of free fatty acids (FFA) in milk. Further, the change in fatty acid (FA) composition in milk can affect the stability of the product and also the manufacturing process. Both internal and external factors, at farm level or at the dairy plants can affect both FA composition and content of FFA. Milking frequency (MF=number of milkings per cow and day) and the composition of feed are two examples of factors generally performed at farm level. The objective of the present study was to evaluate how FA composition of milk and amount of FFA are influenced by two different ingredients supplemented to concentrate.

The pig industry suffers economical loss due to stillbirths, piglet mortality and low birth weight. This is due to breeding programs that have resulted in larger litters. It has been suggested that these problems are due to the metabolic state of the sow during gestation and the fact that she might not be able to supply her foetuses with enough nutrients for survival and a high and even birth weight. A lot of research has been done to investigate whether body weight and back fat thickness of the sow has any impact on her reproductive traits and if there are any special feed components that improve reproduction. Fibers and unsaturated fatty acids have a positive impact on reproductive traits as long as the sow is fed a sufficient amount of energy and protein during gestation and lactation.

Syftet med denna litteraturstudie var att via litteraturen beskriva nutritionens betydelse för sårläkning. Studien genomfördes som en deskriptiv litteraturstudie där metoden bestod av att söka vetenskapliga artiklar i databaserna Medline, Cinahl och Academic Search Elite. Sjutton artiklar valdes ut. Resultatet visade att patienter med bensår eller trycksår i många fall inte når upp till de näringsrekommendationer som finns. Det visade sig vara vanligt att dessa patienter ligger vid gränsen för att vara undernärda.

In Sweden the climate makes it impossible to feed fresh herbage to cattle during many months of the year. During periods when preserved forage must be supplied it is important that this forage is stored in such a way that high quality is retained, both nutritionally and hygienically. Furthermore, it is essential that losses occurring when handling and storing the forage are kept as low as possible. This study compares storage in tower silos, bunker silos, big bags and bales,estimating dry matter losses as well as losses in metabolizable energy and crude protein that occur during storage. The results show that tower silos, bunker silos and big bags, the systems with the largest amount of forage and a long feed out period involve larger losses than bales, which are fed out immediately after being opened. As the feed out rate influences the losses, tower silos and bunker silos should be used during the period of the year when the feed out rate is as high as possible, i.e.

Det är viktigt att elever i skolan äter tillräckligt mycket mat under skoldagen så att de orkar hålla koncentrationen uppe och kan prestera bra i skolan. Syftet var att undersöka om innehållet av energi (kcal), protein, fett och kolhydrater är tillräckligt i maten som elever i årskurs 3, som är på fritidshem före och efter skolan, äter till frukost, lunch och mellanmål under en skoldag. En kartläggning gjordes över vad åtta elever åt under tre måltider under en skoldag och intaget energi- och näringsintag beräknades i relation till Livsmedelsverkets rekommendationer. Resultatet visade att elevernas energi- och näringsintag var bäst under frukosten, även om det var lågt hos de flesta. Under lunchen minskade energi- och näringsintaget och blev ännu sämre vid eftermiddagens mellanmål.

Pyodermi är en hudsjukdom hos hundar som orsakas av Staphylococcus pseudintermedius (S. pseudintermedius), tidigare benämdes den S. intermedius. Pyodermi kan ge många olika symptom såsom klåda, håravfall, rodnad, varigt och blodigt exsudat, papler, pustler, sårskorpor och nekrotiserad hud. Ofta finns det bakomliggande orsaker och sjukdomar hos hunden som tillåter stafylokockerna att kolonisera huden. Staphylococcus pseudintermedius är en Grampositiv kock, den är koagulas-, DNas-, katalas- och ureaspositiv och ger dubbelhemolys på blodagar. Patogenesen vid pyodermi är ännu inte helt kartlagd.

Organic rearing of broilers is not a large production in Sweden. In order to create a sustainable yet efficient production of organically produced poultry meat, at a price that the consumers are willing to pay, much more knowledge is needed. It is therefore important to do more research on organic broilers under Swedish conditions. This report is aimed to be a base for further studies. In the study 2000 broilers of the hybrid Ross 308 divided in to two equal groups. The broiler hybrids that are used in Sweden are selected for a high growth rate and are normally slaughtered at the age of 5 to 6 weeks, with a slaughter weight of 1.7-2 kg.

Leber´s hereditary optic neuropathy (LHON), a disease affecting vision, is caused by several point mutations in mitochondrial DNA. Mutations leading to a defect NADH ubiquinone oxidoreductase protein will affect the respiratory chain and cause a disturbed ATP production. It is still unknown why this defect leads to the degeneration of retinal ganglion cells and cells in the opticus nerve as well as demyelination of axons in these areas. Analysis of mitochondrial DNA is an important tool in the diagnosis of the disease. At the present time analysis is based on cleavage by restriction enzymes, which only detects two of the most frequent mutations: m.3460G>A and m.11778G>A.

In vitro-test för upptäckt och behandling av tumör eller mikrometastaser har de senaste 30 åren gjort stora framsteg tack vare immunokemi och nya framgångsrika cellodlings- tekniker som bättre reproducerar celltillväxt i tre dimensioner (3D) och det omgivande stromat (multicellulär tumörsfäroidodling). TS1-218 scFv (single chain variable fragment) är en monoklonal antikropp som har affinitet till ett protein tillhörande cytoskelettet (cytokeratin). Av TS1-218 har skapats olika varianter (en dimer TS1-2182 och en mutant HE1-Q) med syftet att öka affinitet och retentionstid på platsen för dess verkan. I det här projektet försökte vi att testa och jämföra egenskaper hos alla 3 joderade antikropparna genom att inkubera odlade Hela Hep 2 tumörcellssfäroider med dessa antikroppar. Alla tre antikroppsvarianter visade god förmåga att penetrera sfäroider och att binda deras epitop i cytokeratin 8.