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25 Uppsatser om Staining - Sida 2 av 2
Borrelia burgdorferi : metodutveckling och tillämpning avseende odling och resistensstudier mot komplement, särskilt interaktion med faktor H
The genus Borrelia is a widespread, pathogenic pest and the causative of among others borreliosis or Lyme disease. The vector for the bacteria is the hard tick, Ixodes ricinus and the infection is transferred through a bite. Untreated, Borrelia may cause arthritis, heart damage or neuroborreliosis. Infection is made possible through different strategies for avoiding the body?s complement system.
Comparison of Different Electrophoretic Methods for Haptoglobin Phenotyping and an Investigation in Patients with Abdominal Aortic Aneurysm
Haptoglobin is an acute phase protein with important biological role because of its capacity to bind to haemoglobin. Haptoglobin exists in three major genetic polymorphism types: Hp1-1, Hp2-1 and Hp2-2, the distribution of which has been associated with abdominal aortic aneurysm (AAA), an asymptomatic aortic disease common among men older than 65 years. Five different electrophoretic methods were tested according to their ability to separate the haptoglobin phenotypes. The detection was based on a produced hemolysate of blood in which haemoglobin binds to haptoglobin thereby forming a complex that can be detected by specific haemoglobin Staining using TMB-dihydrochloride and hydro peroxide as substrate resulting in an azure-green color of the bands. Samples from 15 patients who had suffered surgery for not broken AAA, that is more than5.0 cmaortic diameter, and 15 samples from matched controls were analyzed. Among the five tested electrophoretic methods best migration and separation was seen on the pre-cast agarosgel Hydragel HR on the instrument Hydrasys. The other four methods gave less successful results.
Immunohistochemical detection of thymidine kinase 1 in canine mammary tumors and lymphomas
The dog has often served as a model animal for humans in scientific studies. This is due to the fact that dogs are susceptible to a wide range of diseases which also affect humans. One example is cancer, a condition that affects dogs as well as owners. Neoplastic diseases account for 18-23 % of all deaths in dogs (Bonnet et al., 2005 and Jagielski et al., 2002).
Mammary tumor, or tumor of the breast, is the most common tumor form in intact dogs and women (Im et al., 2013). Mammary tumors originate in the udder, and are classified according to their histological characteristics.
Aortic Body Tumors in Dogs
The purpose of this essay was to review the literature and describe the patophysiology, diagnosis, microscopic and macroscopic appearance of aortic body tumors in dogs. The tumors originate from the chemoreceptor organs situated at the base of the heart. The chemoreceptor organs are neuroendocrine cells responsible for surveillance of changes in e.g. the blood pH. Aortic body tumors are rare and represent 7 % of the total cases of primary cardiac tumors in canines.
Genetic diversity of Roseroot (Rhodiola rosea L.) from Sweden, Greenland and Faroe Islands
Roseroot (R. rosea L.) has gained more attention the last years, mainly because of its phy-topharmacologic properties. Even though many reports in roseroot have been published, only a few articles are dealt with the genetic diversity. The Nordic Genetic Resource Center has collected Roseroot material from Sweden, Greenland and Faroe Islands under different expeditions. The samples were analysed to study the genetic diversity study.
Utvärdering av ett kortare dehydreringsprogram för stansbiopsier från human hud och portio : Har storleken betydelse?
På histopatologiska laboratoriet vid Länssjukhuset Ryhov i Jönköping är dehydreringsprocessen i dagsläget förlagd över natt. För upprätthållande av god kvalitet på diagnos med förbättrat provflöde och utnyttjande av instrumentens fulla kapacitet önskas att ett kortare dehydreringsprogram implementeras och förläggs dagtid. Syftet med denna studie var således att utvärdera ett kortare dehydreringsprogram för stansbiopsier från human hud och portio. Detta har gjorts utifrån cellmorfologiska bedömningsgrunder och bedömning av färgkvalité av rutinfärgning med Hematoxylin och Eosin samt specialfärgningarna polykrommetylenblått enligt Unna, Periodic acid-Schiff och Giemsa. Ett material om 87 biopsier från human hud samt portio dehydrerades fördelat över gällande rutinprogram samt utvärderat kortprogram.
Infärgning av matematik i byggprojekt
Med gymnasiereformen 1994 fick alla elever en gemensam första kurs i matematik, kurs A, och ett gemensamt nationellt prov på kursen. På de flesta yrkesförberedandeprogrammen är det över 40 procent av eleverna som inte får godkänt på detta kursprov och 30 procent av eleverna förväntas få ?Icke godkänt? i kursbetyg. En av de största anledningarna, till att eleverna lämnar gymnasieskolan utan fullständiga betyg, är bristen på motivation. Det finns därför ett stort behov av att se över vilka förändringar som kan utföras i de teoretiska ämnena för att öka elevernas motivation och självförtroende på de praktiska programmen.
Frystorkning Kontrollerad torkning av kulturhistorisk dräkt?
Cultural objects may become wet by deliberate water cleaning, water disaster or watersoaked for other reasons. Air drying textile objects and even other organic materials from awater wet state, involves many problems depending on the strong capillarity action of water.Damages and changes such as bleeding of dyes, stains or lines of impurities from watermovement, shrinking or stiffness can occur. Freeze-drying as an alternative drying methodhas often been used for wet archaeological and marine objects, but more seldom for historicalmaterials.This bachelor thesis addresses the issue of whether freeze-drying can be an alternative controlleddrying method for historic costumes. Costumes and related objects are often compositeobjects, consisting of multiple materials, as well as being three dimensional, all of whichwill complicate drying. The purpose of this study was to investigate whether it is possible tohandle costume objects after wet cleaning for drying by freeze-drying, and if damages due tosurface tension of water, can be avoided in this way.
Förekomst av leukocyter i råsperma hos semingaltar :
Detection of leukocytes in semen from boars is part of the routine control of semen quality. Leukocytes in semen can cause damage to spermatozoa that leads to impaired fertilization. In the artificial insemination (AI) industry there are standard routines for the quality control of the boars and of the neat semen before use. Since 2001, Quality Genetics has not done the leukocyte examination on semen samples from boars which are used for the production of pigs for slaughter.
The purpose of this study was to investigate the occurrence of leukocytes in semen from AI-boars. The questions addressed were whether there are boars that have leukocytes in their semen and how frequently it occurs and, moreover, if there are differences between breeds, or even between individuals whose semen was checked before use in AI-dose production compared to those which were not.
The study included a total of 135 AI-boars, of which 67 were Hampshire, 35 Yorkshire and 33 Landrace, all belonging to Quality Genetics AI-station in Hållsta.
En tvättrådsetikett - Dess kvalitetsegenskaper och miljöpåverkan. En litteraturstudie & kvalitetstestning av textila etiketter
Tvättrådsetiketter är en relativt liten detalj på plagg, som dock har en stor betydelse för plaggets livslängd och dess miljöpåverkan. De flesta har nog upplevt att en tvättrådsetikett har blivit oläslig i ett plagg. Det är viktigt att etiketten förblir läslig under hela plaggets livslängd, för att skötseln av plagget ska utföras på ett korrekt sättoch med en så liten inverkan på miljön som möjligt. Därför har kvalitén på ett antal etiketter av olika material och olika framställningsmetoder testats. Det för att sevilken av dessa som är det bästa alternativet kvalitets- och miljömässigt.