In this project a LC-MS-method for the analysis of gamma-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, amphetamine and methadone was developed.Initially, the efficiency of the ionisation of the analytes was evaluated with respect to the ionisation technique (ESI, APCI and APPI) and the composition of the mobile phase. In the next step a number of different columns was tested in order to find the one with the greatest potential for separation of the substances in question. Using the selected column, the separation was optimised by means of experimental design and the software The Unscrambler 7.8. The parameters studied were the flow rate, the column temperature and the mobile phase composition. The response variables were the resolution between the target compounds and the retention time of the last eluting compound.These experiments showed that, in order to obtain the best ionisation, the mobile phase should consist of 5 mM formic acid in water and acetonitrile.

Storage of biomass is often associated with problems such as heat development, dry matter losses and reduction of fuel quality. The rise in temperature can potentially cause a risk of self-ignition in the fuel storage. Moreover, emissions from storage piles can cause health problems in the surrounding. The dry matter losses and reduction of fuel quality can have economical effects. The aim of this thesis project is to develop guidelines on how to store large amount of biomass at Vattenfalls heat and power plants in an optimal way.

The purpose of this study is to describe melanotic tumours and the pathogenesis of these tumours. The focus of this study is on differences between benign and malignant tumours as well as the prognosis one may expect when diagnosing this disease. The mechanisms responsible for the occurrences of the tumours are not yet fully understood. It is believed that factors such as chronic trauma, infections, medications and hormones affect the cell mechanisms in some way. Determinants of the malignancy of the tumour are not completely known.

The purpose of this study is to describe melanotic tumours and the pathogenesis of these tumours. The focus of this study is on differences between benign and malignant tumours as well as the prognosis one may expect when diagnosing this disease. The mechanisms responsible for the occurrences of the tumours are not yet fully understood. It is believed that factors such as chronic trauma, infections, medications and hormones affect the cell mechanisms in some way. Determinants of the malignancy of the tumour are not completely known.

BACKGROUND: Pressure ulcers are unpleasant and painful and causes anxiety not only patient but also for the relatives. It also results in longer hospital stay and increased costs for health care sector and because of this it?s important to identify and survery the origin and what preventive measures there are to prevent pressure ulcer. AIM: To illustrate the nurses´ work to prevent the origin of pressure ulcers. METHOD: A general literary study where scientific articles were searched and found in electronic databases and through manual searches.

The aim of this study was to determine a method to habituate treadmill-naive Labrador Retrievers to walk and trot on a treadmill. 17 Labradors was equipped with reflective markers glued to the skin. Their motions were captured with six 240 Hz infra-red cameras as they walked and trotted on a treadmill three times a day for two days. Also the heart-rate was recorded. We then used QTM and custom built software to analyze the measurements.

In developing countries such as Vietnam the population consumes more animal products for example milk and meat than before which requires greater livestock production. Ruminants contributes to more methane emission which creates a dilemma between food production and its environmental impact. By feeding ruminants with nutritive crops which humans can not assimilate the animal performance will increase in terms of better growth and milk production without inpinging on food that can be consumed directly by humans. Increasing animal performance reduces methane emission in terms of amount of methane in kg-1 milk and meat which today is much greater in developing countries than developed countries. The aim of the study was to investigate tropical tannin-rich legumes for their potential as a feed supplement for ruminants made in vitro.

A titania-supported nickel catalyst was prepared and tested in methanation in order to evaluate its catalytic properties (activity, selectivity and specially, activity loss), and compare it with an alumina-supported nickel catalyst.The titania-supported catalyst did not only show higher stability than alumina, but also presented a different cause of deactivation, carbon formation. In addition, a kinetic model was obtained for the titania-supported catalyst, and a study of the effect of different operating conditions (temperature, composition and partial pressures of synthesis gas and water) on the deactivation rate and carbon formation of this catalyst was performed.

Food-poisoning is a major health problem and an estimated half a million Swedes are food-poisoned annually, with acute gastroenteritis as a consequence. One of the major causes of contaminated foods is related to food- and waterborne viruses. To be able to trace back the source of contaminant, the method of detecting viruses must be specific and sensitive. No standardized method for detecting foods for sapovirus exists today. The aim of the work described in this bachelor thesis is to implement and opti-mize a real-time RT-PCR method for the detection of all genogroups of human sapovirus in foods.

Arvika Fastighets AB is a municipal subsidiary tasked with managing all green areas in the municipality, with a strong focus on the City Park and plant beds in the city centre. These areas generate large amounts of green waste, which is currently transported to the municipality's composting area at Lycke. Currently, the material is piled up and turned once a year without using the resulting soil for flowerbeds, lawns, and shrub/tree plantings. The purpose of the study is to determine if Arvika can compost the green waste in a more efficient manner and produce weed-free soil ready to be mixed into various substrates for different purposes. Interviews with the Swedish Church and several municipalities in Sweden have been conducted to learn from their experiences and knowledge of how they make their composting work.

Bakgrund: År 1991 lanserade WHO och UNICEF "Baby Friendly Hospitals Initiative" (BFHI). Motivet var den nedåtgående amningsfrekvensen världen över och de konsekvenser som det medförde till exempel socialt och ekonomiskt. Till grund fanns innocentdeklarationen som bygger på att alla BB avdelningar skall följa "tio steg till lyckad amning. Målet med detta är att främja, stödja och skydda amningen. Forskning visar att amningsfrekvensen har sjunkit de senaste åren i Sverige.Syfte: Syftet var att undersöka nyblivna mödrars upplevelse av stöd i sin förberedelse och uppstart av amning i Kronobergs län och jämföra detta med mödrar vid tre andra kvinnokliniker (Blekinge län, södra Kalmar län och norra Kalmar län).Metod: Undersökningen har en kvantitativ ansats.

Rabbits, Oryctolagos cuniculus, rapidly increases as pets. During the right circumstances, rabbits are friendly, social and playful. A rabbit who shows an abnormal behavior should always be examined by a veterinary, though there is almost always a medical problem. This essay is a result of a literature studie with the aim to find the prophylactic measures that may give pet rabbits a more healthy life. Rabbits have got a lot of common health problems, including dental, skin and gastrointestinal problems.

Atopic dermatitis (AD) is a complex, often lifelong allergic disease affecting around 10 % of both dogs and humans. The hallmark symptom is severe pruritus, causing a lowered quality of life. Mast cells (MCs) are known to play an important part of the immunopathogenesis, promoting a faulty T helper cell type 2 (Th-2) response which follows by a production of specific immunoglobulin E (IgE) antibodies towards environmental allergens (Ag). To further investigate the role of MCs and its mediators in the progression of AD, a low-calcemic vitamin D3 analog (MC903) was used to induce AD-like symptoms locally on the ears of two different knock-out (KO) mouse strains. The first strain was Wsh-/- mice deficient in MCs.

During the 19-century a global warming has been observed, which includes increases in global air and ocean temperatures, widespread melting of ice and snow, and rising global sea level. There is a clear connection between emissions of greenhouse gases caused by the human and the increase in temperature. Climatic changes caused by global warming can be stopped trough decreased emission of fossil fuels, for example by an increased use of biogas. Biogas is a renewable energy source which is produced through anaerobic (oxygen free) digestion of organic material. The gas is a mixture of methane (CH4) and carbon dioxide (CO2) and can be among others used as fuel in vehicles.

Swedish farmers have the possibility to be subsidized by EU rural development programme for grazing animals on pastures to promote the biodiversity of flora and fauna. The environmental remedy sometimes does not allow supplementary feed and at the same time the Swedish Animal Welfare Act and the Swedish Agricultural Board?s regulations for animal welfare and protection states that animals, in this case horses, shall be fed with food of good quality and adapted for the species. The regulations also state that they should have an individual feeding regime that enables them to maintain a normal body condition. The aim with this master thesis has been to investigate if it is possible to keep Gotland ponies on extensive pastures, during late spring and summer and still maintain animal welfare. The horses were assessed every fourth week using a welfare assessment protocol developed in line with the Welfare Quality® project (submitted, Viksten et al) where physical, behavioural and resourcebased parameters were observed. Twelve one-year old stallions of the breed Gotland ponies purchased from different breeders were used in the study.