In situ sekvensering är en metod som kan användas för att lokalisera differentiellt uttryck av mRNA direkt i vävnadssnitt, vilket kan ge viktiga ledtrådar om många sjukdomstillstånd. Idag förloras många av sekvenserna från in situ sekvensering på grund av det kvalitetsmått man använder för att säkerställa att sekvenser är korrekta. Det finns troligtvis möjlighet att förbättra prestandan av den nuvarande base calling-metoden eftersom att metoden är i ett tidigt utvecklingsskede. Vi har genomfört explorativ dataanalys för att undersöka förekomst av systematiska fel och korrigerat för dessa med hjälp av statistiska metoder. Vi har framförallt undersökt tre metoder för att korrigera för systematiska fel:I) Korrektion av överblödning som sker på grund avöverlappande emissionsspektra mellan fluorescenta prober.II) En sannolikhetsbaserad tolkningav intensitetsdata som resulterar i ett nytt kvalitetsmått och en alternativ klassificerare baseradpå övervakad inlärning.III) En utredning om förekomst av cykelberoende effekter, exempelvisofullständig dehybridisering av fluorescenta prober.Vi föreslår att man gör följande saker:Implementerar och utvärderar det sannolikhetsbaserade kvalitetsmåttetUtvecklar och implementerar den föreslagna klassificerarenGenomför ytterligare experiment för att påvisa eller bestrida förekomst av ofullständigdehybridisering.

Ground construction has to a large extent been dominated by traditional meth-ods during the last 50 years. Although the construction has been changed meanwhile innovations are not accepted. In collaboration with NCC Ltd Region South, I have examined a modern con-struction methodology that has been developed by NCC for future large pro-jects. The method is divided into two central phases. Phase 1 is? Single Pro-duction? which is a preliminary period for ground constructions to come.

The main purpose of this essay is to identify opinions of masculine and feminine language in a written text. The aim is to examine whether the reader of a text can define the anonymous author?s sex, judging only by elements such as choice of words or sentence structure. Are there any differences between the sexes as they appear in written language, and is the common man able to make this distinction? The essay is based on several studies on people?s ideas on masculine and feminine language, as well as studies on actual differences.

The purpose of this study was to explore if and how the Swedish Supreme Court are using the concept ?the child?s best interest?. This concept is used a lot in Swedish legislation, but it needs interpretation every time it´s used and is often perceived as unclear. This makes it interesting to see how this affects the individual child´s legal rights. Furthermore I want to see if the Supreme Court listen to the child´s own opinion and if not, are there any justifications to why not.

Today digital guides are most frequently used by the museum and tourism industries. They are also used for education purposes. They make it possible to distribute information and have it easily accessible. In addition to written information they add sound, pictures and videos which lift the whole impression of the visited place to a higher level. A variety of systems currently in use today have been evaluated.

The aim of this study was to understand how evaluations are implemented and used in procured nursing homes for elderly. Our methodological approach consisted of a single-case study research. The case in this study was Växjö municipality. Three procured nursing homes for elderly were chosen in the municipality as examples of implementation and use of evaluation. The method for retrieving empirical data and the analyzing of it was based on triangulation.

AbstractThis essay has as its purpose to discuss the current legal regulation of the concept of humanitarian intervention. The inconsistencies in the debate over the legal status of this concept, and the legal uncertainty it brings to the acts of states and the lives of their nationals is a motivating factor for the writing of this essay. However, it has been clear from the outset that the concept of humanitarian intervention is intricately connected to political and moral ideas and values. Thus, the attitude taken towards this doctrine will be highly dependent on the perspectives of the state, government or single author representing it.Following this starting point, the aim of this essay is not to present a single answer as to whether humanitarian intervention is, or is not, legal, but to research, compare and analyze the different arguments put forward in this subject in international law today. Hopefully, this will provide the reader of this essay with some insight into the sources of international law of today and how the principles of state sovereignty, non-intervention, the prohibition of force and the protection of human rights relate to the concept of humanitarian intervention.A frank overview of the UN Charter does not support use of force except in the case of self-defence or without a Security Council mandate.

Old people's life situation when receiving municipal help and care in theirlast period of life is sparsely investigated from their own perspective. Thepurpose of this study was focused on the thoughts of the aged people andtheir personal experiences on what quality is within the geriatric care. Inthis qualitative study, 10 elderly people aged 75-90 years wereinterviewed from 3 different nursing homes within Solna Municipality.Qualitative interviews, with the emphasis on their present life situationespecially what brought about a good life, were performed. The interviewswere analysed using qualitative content analysis. The implication of theterm ?meaningful existence? is individual and differs from person toperson.

With examples from a single classroom situation, several educational moments show what goes on between the teacher and the student. The teacher?s responsibility in the learning process is, with a gesture frozen in the air of the moment, to lead and follow the student in her tentative efforts at seeking knowledge. Initially, it is an attempt to shed light upon the "not yet seen" with a certainty that the student herself possesses the capacity and the aptitude to reach greater insight and clarity in her understanding. The Validation/Feil Method presented in this instructional situation illustrates how best to communicate with elderly people suffering from Alzheimer?s dementia.

The world is getting smaller. Global markets, political processes and communication technology bring people together. Even though many of these processes are dynamic and constructive, they have also resulted in a need for better and more effective ways of international conflict resolution. This thesis investigates central issues connected to the concept of humanitarian interventions in relation to current and future legislation of the European Union. The overall question is whether the rapid globalization process has resulted in a change of international law towards more liberal view where authorization of the security council is not always needed? And, if there has actually been such a liberalization, how does that affect the legal possibilities for the European Union to carry out armed operations without the consent of the global community? The conclusion of the thesis is that international law has been modified mainly in two aspects: First, the concept of sovereignty is no longer seen merely as a right of the nation, but also as a responsibility.

The aim of this two year master thesis is to study the knowledge production within the field of Textile Studies at Uppsala University, with special focus on the use of artifact documentation as a research method. With domain analysis as a theoretical framework, ontological and epistemological assumptions as well as historical and social aspects are studied in relation to the research process. Qualitative research methods are used, including interviews, observations and text analysis. In the analysis of the knowledge processes in the field, perspectives on tacit knowledge and the concept of documents, are used as a theoretical basis. In the field of Textile Studies, the artifacts are used as one of the main objects of study, along with archival and pictorial sources. The main epistemological assumptions are that artifact analysis is dependent on a knowledge base, where technical handicraft skills, contextual knowledge and practical experience of object observation are the most important qualifications.

In this thesis I have used post-colonial theory to analyze the Uyghurs, a Muslim minority group located in Xinjiang province in the Northwest of China, and their identity shaping process in relation to the dominating Han Chinese. I have analyzed the possibilities to use the power perspectives in post-colonial theory, especially Edward Said's Orientalism and the creation of the Other in a new context, where the main focus is on the Uyghurs through the eyes of Han Chinese. I have argued that Han Chinese, with colonial tendencies, have created two generalizing pictures of the Uyghurs; one romantic picture to use as an exotic tourist attraction and one threatening picture that portray Uyghurs as Islamic terrorists to win support in international politics. The thesis is a qualitative single case study with the aim of gaining a deeper understanding for the Uyghurs as well as examine possibilities of widening the analyze areas for post-colonialism. My conclusion is that the power perspectives in post-colonial theory are usable implements also for non-western contexts and in this case function as a way to understand the identity shaping process for the Uyghurs and their relation to Han Chinese..

In this thesis I investigate aesthetic preference for picture books among children aged 3?6 as well as criteria for preference among parents. Through my design project: illustration of a picture book, I aim to put my research on preference in the context of illustration and design. By using the design project as an applied method for research I have sought to answer how one as an illustrator can apply knowledge regarding preferences of the target groups when illustrating for picture books. I have primarily based my research within the field of cognitive developmental psychology, with primary focus on stage theory, and the field of literature.

A study has been conducted on how organizations in Sweden administer costs of facilities management services internally. There are three main theoretical methods for administering costs. In the first method, costs are maintained on a central level; in the second method, costs are allocated and in the third method, internal transfer pricing is used. The aim of the study is to identify which methods are applied in practice, to evaluate whether the methods fulfill their intended purposes and to study the relationship between the methods used and the cost level of facilites management services. In practice, a large diversity of methods are used for facilities management costs.

Introduction: Good hand hygiene practice is the single most important measure to reduce the spread of bacteria and viruses in healthcare. For nurses, this has become more important as the spread of multidrug-resistant bacteria in hospitals is increasingObjective: To identify factors that are important for adherence to hand hygiene among health care workers with patient contact.Method: Systematic literature review. Original articles were searched in PubMed and Cinahl.Results: Factors of importance for compliance was profession, lack of time, knowledge/education, physical work environment, attitudes toward hand hygiene, and dry hands.Conclusion: More education, less workload and more visible placement of hand disinfectant containers are examples of actions that could lead to the improvement of staff hand hygiene and reduce the incidence of healthcare associated infections. Due to the fact that health care workers around the world have different training and approaches, the result of this study may be difficult to use. To increase adherence to hand hygiene, a similar study limited to a specific country, such as Sweden, would therefore be interesting to carry through.Keywords: hand hygiene, Infection Control, Compliance..