The ability to detect protein-based biomarkers, which are linked to different diseases like colorectal cancer, is very important as a diagnostic tool. Usually complex biological samples like blood are studied which will contribute to different technical issues when performing an assay. The aim with the project is to optimize and develop the high throughput protein detection technique Proximity Extension Assay, PEA, into a 96-plex panel, in hopes of discovering an expression profile for colorectal cancer. PEA was developed by Olink Bioscience and allows specific proteins in a sample to be quantitatively transformed into nucleic acid sequences that are subsequently detected and quantified with real-time PCR. Two proximity probes containing oligonucleotide sequences bind pairwise to target protein and when brought in proximity, a DNA polymerase will extend a hybridization arm from one probe over to the second forming a double-stranded DNA sequence that can serve as a template in real-time PCR.

Children and youth inactivity and sedentary at school is increasing. This means that some of the students have a significant unhealthy lifestyle with physical inactivity, which in turn implies risks of suffering from various diseases, including obesity, type 2 diabetes and cardiovascular disease, all related to a sedentary lifestyle. To prevent illness, it is important that children and young people early get a positive attitude towards physical activity.The purpose of this study was to study the causes of child and adolescent physical inactivity in school, based on teachers 'and parents' perspectives.A qualitative research has been selected. To achieve the study objectives, the author has conducted seven semi-structured interviews, with an appropriate choice of five teachers and two parents with children in school. Data was analyzed with a manifest content analysis.The results show that teachers and parents felt that physical activity is a protective factor for children's health.

The physical inactivity and other diseases related to ways of living in Sweden, affects a big part of Swedes, simultaneously as the society is more defined and marked by health promoting and by views that affects many people to actively make a difference in their lives. Physical activity on prescription is such an example of health promoting, which enlarges the conditions for a healthy life. Physical activity on prescription is also about that the individual should take the decision by herself to increase her physical activity or to get motivated to start a healthy life style alternative reduce the risk of getting sicker. The purpose of our study was to illustrate what the participants of ?fysioteket? thought about the work of fysioteket and if the physical activity on prescription has affected their motivation towards physical activity and their experienced health.

Sweden is getting an aging population and with this comes an increase in neurodegenerative diseases such as Alzheimer?s disease (AD). Bilingualism is also on the rise and this may result in an increase of bilingual people suffering from AD. Due to the linguistic deterioration associated with the illness people with AD, bilingual and monolingual, will be an increasing patient group with speech and language pathologists (SLPs). Word retrieval difficulty is an early symptom of the disease and several strategies to compensate for this have been observed (e.g.

The hybrid larch (Larix x eurolepis Henry) is a fast growing tree species and was introduced in Sweden for about 50 years ago. The high growth and expected resistance to diseases are probably the main reasons of introducing the tree species into the Swedish forests. The purpose of this thesis was to document the production of hybrid larch planted in the Snogeholm landscape laboratory and to compare the production in the pure hybrid larch stand with the mixture together with pedunculate oak (Quercus robur). The data collection in Snogeholm since the establishment has been carried out by the Swedish University of Agricultural Science (SLU). The fieldwork for the thesis was carried out in 3 stands, containing hybrid larch, pedunculate oak and a mixture of hybrid larch and pedunculate oak, in order to be able to compare the total stand production of the areas. The results show that the total production of hybrid larch in the mixed stand only is 35 % compared with the total production of hybrid larch in the pure stand. Therefore, the hypothesis that the total production of hybrid larch is equal to the planted mixture with pedunculate oak was rejected. The other hypothesis that the total production of a 2 hectare large stand containing a mixture of hybrid larch and pedunculate oak would be greater than the total production of two pure stands of 1 hectare containing hybrid larch and oak each was also rejected. In the studied case the total production was 45 % greater if hybrid larch and pedunculate oak was planted in pure stands. The results from the case-study only indicate that the total production of hybrid larch and oak under the investigated conditions can be higher when planting the two species in pure stands compared with a mixture of the two species.

AimThe aim of this study was to find out whether Motivational Interviewing can affect people to change their behaviour in form of increasing physical activity. What results do studies conducted at healthy adults using Motivational Interviewing with physical activity as an outcome present?MethodTo fulfil the aim of the study we chose a review process. During the research two data-bases were used, PUBMED and CSA. Studies including participants under the age of 18 or studies where the participants suffered from diseases were excluded.

Commonly held dietary guidelines discourage full-fat dairy consumption due its to high levels of saturated fatty acids, which are believed to aversely influence cardiovascular disease risk. Cardiovascular diseases cause more than one third of deaths in the developed world. With dairy often providing a large part of energy and nutrients in Western diets, research into its effects on human health is warranted. Several recent studies have suggested a protective role of dairy in the development of cardiometabolic disease. However, many of these have used imprecise methods of dietary assessment, based on questionnaires or interviews. Biomarkers are emerging as a means of evaluating diet in a more objective way. Currently used dairy fat biomarkers are minor fatty acid constituents of dairy that preferably are unique to dairy and not influenced by endogenous metabolism.

Background: The majority of the occupational accidents and work-related diseases at Swedish workplaces occur due to poor ergonomics during lifts and transportation of objects. The most common consequence of poor ergonomic conditions is low back pain (LBP) and the risk of developing LBP increases as the mechanical loading on the intervertebral disks grows. This study has investigated the biomechanical loading in the low back during two different types of patient lifts. The first lift (lift A) is developed by Modern Arbetsteknik (MA) and the second lift (lift B) is a common lifting technique among care workers. Objectives: The aim of the study was to identify any advantages or disadvantages with MA?s lift, regarding shear and compression forces in the L5/S1 disc.

COPD (Chronic Obstructive Pulmonary Disease) is one of the most widespread lung diseases today and is a growing cause for suffering and mortality worldwide. It is considered to be the third leading cause of death in the near future. Smoking is the most important risk factor for this disease and about 50% of smokers develop COPD. The purpose of this study was to mediate the experience of not being able to stop smoking described by persons diagnosed with mild or moderate COPD. The study involved five women and five men.

The progress of the health among the elderly has a major meaning for the needs of care, nursing and social services. The health of the elderly has improved but fall and fall accidents are a major problem. Acute diseases, activities and environment risks are often related to fall accidents. The aim of the study was to describe health care staffs? opinions about fall risk and fall prevention.

Trees in urban environment are exposed to extreme conditions, foremost the ones that grow in the hard packed soil along our streets. They are also exposed to damaging insects and fungal diseases both local and new ones spreading from South Europe. The global warming with warmer temperatures provides a longer growing season, making it easier for insects and fungus to establish a habitat. Based on these facts new tree species need to be available for future urban plantings.To determine what these trees would be an interview study began. Six people were selected who have experience in the field of urban environment planting.

Background: Cancer disease is one of the most common diseases in Sweden andradiotherapy is one of the regimens available for the treatment of cancer. Radiotherapycan be delivered as single treatment or be divided into several fractions that last forweeks. Radiotherapy can cause side effects that may occur during or at the end oftreatment. Information and communication is an integral part of all treatments and care.The oncology nurse should attempt to ease for the patients to communicate, participatein the care, receive information and enhance the ability to self-care.Aim: The aim of the study was to examine the patients? needs for communication andinformation with oncology nurse during their meeting through radiotherapy.Method: This pilot study was conducted on six patients with different cancer diagnosis.It was a qualitative study, were data was collected through individual interviews usingsemi-structured questions.

The Swedish government published their current proposition concerning cultural politics,Tid för kultur in 2009. Due to this proposition, culture is considered a basic human right we all deserve to be provided with. It contained goals and guidelines for how to enable culture to all citizens, to integrate and enrich the state, the county councils? and municipalities? work with diversity and multi culture in their culture-based institutes. However, there seems to be a lack of strategies in the issue of how to provide our nondependent elderly, suffering age related handicaps and diseases, with this proclaimed necessity.In recent years, there has been a lot of research proving the link between human wellbeing and a stimulating environment.

The purpose of this essay is to examine wage-earners' investment funds from the ideological point of view. Were they in any way an integrated part of social democratical democratic socialism and reformism? I emphasize Olof Palme´s ideological idea of democratic socialism and reformism, and how he handled the issue. How did the question of these funds correspondent with the basic ideological points of view, and what was the standpoint of Palme in this issue.My method is built upon a deep study and analyses of SAP board of party and the standing committees protocol in the light of Olof Palme´s and SAP's ideology. I even use information from literature, inquiries and dissertations.

For centuries inbreeding has been used to refine traits in dogs, making desired traits more hereditary and thereby, different breeds have been invented. Breed standards have been written for each of these breeds, describing in detail the different characteristics of each breed. When humans start to choose which animal to breed from, so called artificial selection, taste is put before function and the laws of nature, the natural selection, cease to apply. During this development, pedigree dogs have gone towards more and more extreme exterior characteristics and the dogs may have inherited various, more or less, severe problems for example compromised vision, impaired ability to move and difficulties to breathe. However they can still survive and reproduce because humans provide them with the conditions to do so.