Endotoxinemi hos häst ses framförallt i samband med sjukdomar i mag- och tarmkanalen. De metabola rubbningarna och den utbredda inflammationen som endotoxinemin medför, gör att det är ett allvarligt tillstånd som kräver veterinärvård. Förändringar i aminosyrametabolismen har setts hos människor med experimentellt inducerad endotoxinemi och patienter med sepsis. Den kraftigt ökade muskelnedbrytning som endotoxinemi medför anses ha stor betydelse för patientens återhämtningsförmåga och prognos för överlevnad. Förutom aminosyrornas egenskaper som byggstenar för produktion av protein har de en viktig funktion för cellsignalering, bidrar till bevarandet av ett fullgott immunförsvar och hämmar cellskador.

Glutathione (GSH) is a molecule that consists of three amino acids: glutamic acid, cysteine and glycine. GSH has several important functions: to protect cells from free radicals, reactive oxygen species and oxidative stress. GSH exists in a reduced form, GSH, and in an oxidized dimeric form, glutationdisulfid, GSSG. The enzymes glutathionereductase (GR) catalyses the reduction of GSSG back to GSH. Nicotinamide adenine dinucleotide phosphate (NADPH) is required as a coenzyme in the reaction.

Proteins are vital to all cells in the body. They consist of long chains of amino acids. To be able to study the amino acid composition of a protein it is necessary to hydrolyse it, followed by separation and quantification. When the protein is hydrolysed, in this case ß-lactoglobulin, the protein is divided into individual amino acids. The method that traditionally has been used to hydrolyse proteins takes 24-72 hours to complete.

En metod för detektering och kvantifiering av gamma-hydroxysmörsyra (GHB) i urin med gaskromatografi (GC) är framtagen på Sahlgrenska universitetssjukhuset. Metoden är relativt unik då den inte kräver upparbetning i form av derivatisering, indunstning eller extraktion. Urinen surgörs med koncentrerad saltsyra och internstandard, gamma-valerolakton, tillsätts. GHB övergår då till laktonformen, gamma-butyrolakton (GBL). Därefter injiceras provet direkt på en GC-FID med en kapillärkolonn för glykoler och alkoholer.

Proteins are vital to all cells in the body. They consist of long chains of amino acids. To be able to study the amino acid composition of a protein it is necessary to hydrolyse it, followed by separation and quantification. When the protein is hydrolysed, in this case ß-lactoglobulin, the protein is divided into individual amino acids. The method that traditionally has been used to hydrolyse proteins takes 24-72 hours to complete.

In southern Sweden, beech (Fagus silvatica) and spruce (Picea abies) are two species with similar demands on soil properties. With increased global warming and high acid deposition the future may bring larger areas were the two species are overlapping, competing for the same soil. The aim of this study was to compare spruce and beech concerning distribution, cultivation and soil properties. From available data comparisons were made between spruce and beech forests in Sweden. Soil analyses were made on samples from beech forest in southern Sweden, with and without natural regeneration of spruce.

Tripeptidyl peptidase II (TPPII) is an exopeptidase which cleaves tripeptides from theN-terminus of peptides. The exact functional role of TPPII is still a matter of investigation. Itis believed that the enzyme is primarily involved in intracellular protein degradation, where itcooperates with the proteasome and other peptidases to degrade proteins into free aminoacids. These amino acids can subsequently be used in the production of new proteins. The aimof this work was to express murine wild type TPPII using E.

Hundreds of thousands of piglets are castrated without anesthesia in Sweden every year so that we may be guaranteed meat without boar odor. The smell appears while cooking meat of boar and is described as urine- and sweat-like. High levels of androstenone and skatole are considered the main factors behind boar odor. Skatole is a metabolite of the amino acid tryptophan, which is formed in the colon of all pigs. Androstenone is a steroid hormone that is released from the Leydig cells in the testes of boars.

It is possible to affect the feed costs in different ways. By steeping or fermentation the digestibility of the feed is improved for the animals with a natural process in the wet feeding system Steeping means that the feed is soaked some hours before feeding. This process activatesenzymes and improves the digestibility of for example phosphorus in the feed and make it more accessible to the animal. Wet feeding can be fermented or not. Fermentationmeans that the feed has been soaked for a certain length of time so that a biological process is started.

This thesis raises the problem with acid mine drainage around the closed mine Finngruvan.The purpose of the investigation was to compare metal content in surface water in the areaand its catchments between year 2010 and 2003, also to make calculation of specific runoffand metal load, furthermore determine the recipients in the area. Surface water samplingwhere preformed at the same place as 2003 and also at a few other places of interest. Adigital elevation file were used in GIS to calculate the area of the watersheds in the region,observations in the field where also made. The collected data were used to make comparisons,calculations and conclusions. The result showed that the surface water 2010 had somewhatlower metal concentrations.

This report describes a new foundation method and compares it with a traditional founding of concrete. This new method is called the Koljern technique and uses cellular glass which is called Foamglas. The Foamglas is compounded to founding elements by lightweight steel beams. Foamglas is an insulation material with high compressive strength, and is rot, insect, vermin and acid proof. The cellular glass is also fire proof and watertight, which makes it a very good construction material.The report contains a study of a number of cases for which the Koljern technique is compared with a foundation of concrete.

In the year 1783 a fissure eruption from Laki in the southern part of Iceland produced a large amount of volcanic gases during a period of eight months. The volcanic gases and aerosols spread across the northern hem sphere due to ideal weather conditions and had a substantial impact on the environment, human health and also climate effects. This, so called dry fog, caused severe health problems, which led to death casualties, killing animals, crops and other vegetation. The consequence was a widespread famine which was aggravated by a hard and long winter in 1783/84. Analysis shows that the Icelandic volcanoes are the primary risk to produce dry fog that can affect Sweden.

The mining industry, in terms of ore excavation and metal production, is and has been important to the Swedish economy. In connection with the ore excavation process, large amounts of partly sulphidic waste are produced. When the metal sulphides are exposed to air and water they are eventually oxidised, which leads to the formation of acidic water with high concentrations of metal cations and sulphate ions, so called Acid Mine Drainage (AMD). This water may reach the nearest surroundings and cause negative environmental effects. By covering the waste with soil or water, the oxidation process can be minimised. At the Rävlidmyran mine in the county of Västerbotten open pit mining took place between 1951 and 1991.

It is important that the current urine separating systems are evaluated to allow improvements to be made and consequently increase their future propagation. In order to increase the knowledge about urine separating systems this study was made. It included, among other things, measurements of amounts of urine as well as of the composition of urine collected at Nytorgsgatan 4 in the city of Hallsberg. The study was increased after an inquiry that also included the district of Understenshöjden in the city of Stockholm. In the inquiry, the users were asked questions about odour, cleaning of toilets and occurrence of blockages in the urine water locks etc.

Since several lifestyle diseases are associated with obesity much research is carried out within the area. Researchers have found that oxidation of fatty acids within the body?s cells may give rise to peripheral signals which regulate food intake, but it is still unclear where such a mechanism takes place. Although much evidence suggests that it is the hepatocytes in the liver that send signals regulating food intake, some researchers have begun to question this theory. Instead they argue that the mechanism takes place within the enterocytes since they are well localised in the gut for such assignment.