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180 Uppsatser om Rolling circle amplification - Sida 5 av 12

Investigation of tissue factor mRNA levels in human platelets using real-time PCR

Tissue factor (TF), a 47 kDa glycoprotein, is the initiator of the extrinsic pathway of blood coagulation and consequently of the upmost importance when damage to blood vessel occurs. The source of TF in circulation has been investigated. However, the source of TF is still not clear. One theory is that platelets express and increases the expression of TF after stimulation and the aim of our report was to investigate whether platelets really are a source for TF in circulation.Using specific primers for TF mRNA, platelets in plasma from healthy volunteers and from patients suffering from cardiac infarction were analyzed by using real-time polymerase chain reaction (PCR). Gel electrophoresis was performed after amplification of TF mRNA to verify the results.The samples were negative for TF when using real-time PCR and the few positive all had cycle threshold (Ct) values above 35.

Artefaktualitet, godtycklighet och värdeneutralitet - en diskussion av Dickies institutionella konstteori

Jag ska i denna uppsats redogöra för och diskutera kritik emot George Dickies institutionella konstteori, såsom den framställs i the Art Circle (1984) och framåt, senast i Art and Value (2001). Det vill säga vad som brukar kallas för Dickies senare institutionella konstteori. Jag kommer att begränsa mig till denna senare variant av teorin då det är den enda teori som Dickie fortfarande vill försvara och då den av de allra flesta anses vara den bästa och mest utvecklade versionen av den institutionella teorin. Att jag väljer att diskutera en teori som kan tyckas gammal (om än kanske inte i jämförelse med många andra teorier som fortfarande diskuteras), föråldrad och hårt kritiserad beror för det första på att Dickie (2001) fortfarande håller fast vid den och försöker försvara den. För det andra på att många senare teorier om vad konst är på ett eller annat sätt är inspirerade av eller påverkade av Dickies teori, och det tycks därför som om den fortfarande borde ha vissa styrkor (om än också svagheter).

Folksonomi på webben: indexeringskonsistens och konstruktion av taggar bland Delicious användare

The aim for this master?s thesis is to investigate the indexing consistency in a folksonomy Delicious. This is done by investigating how users construct tags. By applying a semiotic perspective and use analysis of content the occurrences of inflected forms, hyponymy, meronymy and users own tag creations were established. Lancaster?s seven factors is the starting point for the discussion about indexing and the factors that affects it in either a negative or positive way.

Analys av linjetaxering i samband med gallringsinventering med avseende på kvalitet, tillförlitlighet och framtida utformning

Modern forest owners are very familiar with forest management and forest economics. Therefore they have high demands for quality in forest operations. Guarantee of quality in the shape of self-checking activities is an important tool to guarantee a well managed forestry operation. To meet the forest owners? demands on guarantee of quality and the entrepreneurs wish of an easy method for self-checking, the forest owners association, Södra skogsägarna, has developed a new method for thinning inventory. The purpose of this study is to evaluate the method that is used for self-checking and to find ways to develop and improve of the method. The data has been collected by inventory in a number of stands with circle-area survey and with strip survey, which is method that is used for self-checking. The results show that the strip survey does not diverge more than ±10 % in measuring values of basal area, number of stems, roads share, thinning quotient, outtake of basal area and total volume compared to circle-area survey.

Automatiskt Nödstopp

In today?s industry it is starting to get more and more interesting with wireless systems and solutions for applications. To be able to reduce the amount of cables drawn to various machines which takes both space and an extra fee for every cable drawn. Some systems might require the user to use a handheld device that is directly connected by a cable which in some machines might be inelegant for the user.The goal with this bachelor thesis is to develop wireless monitoring of the machine operators position that is working with heavy industrial machinery. The thesis started out with researching different techniques for positioning a transmitter indoors, this was done in order to get a better understanding on how to go about.

Mamma, pappa, barn : En studie om flickor och pojkars lek i docvkrån på fritidshemmet

In this essay, I interview six users from the website darkside.se, which is an internet community for BDSM practitioners. I?m using Judith Butlers theorys about performativity and heteronormativity, Gayle Rubins model the charmed circle; of which sexualities is regarded as approved and which is not, and Fanny Ambjörnssons explenation of queer theory. With the interviews as my material, I investigate how sex and gender is produced and used in BDSM and at Darkside. I discuss trans, heteronormativity, sexism and hierarchies of power related to sex and gender, in BDSM and at Darkside.

Idrottsledarskap i näringslivet   - Lättare sagt än gjort? : En kvalitativ studie av idrottsledarskapets betydelse inom IKEA

In this essay, I interview six users from the website darkside.se, which is an internet community for BDSM practitioners. I?m using Judith Butlers theorys about performativity and heteronormativity, Gayle Rubins model the charmed circle; of which sexualities is regarded as approved and which is not, and Fanny Ambjörnssons explenation of queer theory. With the interviews as my material, I investigate how sex and gender is produced and used in BDSM and at Darkside. I discuss trans, heteronormativity, sexism and hierarchies of power related to sex and gender, in BDSM and at Darkside.

Kartläggning och analys av scanias tillsynsprogram : Ett verktyg för förbättring

Scania intends to introduce a new maintenance program for their rolling fleet. Withthis new program Scania hopes to increase their competiveness and add furthercustomer value. This thesis report is a mapping of the current maintenance program,along with its problematic features. Furthermore the report intends to describe thenew program, Condition Based Maintenance (CBM) and the problems that Scaniamust deal with before they can implement it.The report compiles basic information on how a mapping should be performed.Further on it will describe some of the theories of LEAN and leadership. Which aretwo of the most important features in this thesis work.The work has been performed through visits in different workshops, whereinterviews have taken place.

Erfarenheter av några utfodringssystem till får :

The evaluation of the function of the feeding of sheep herds is suggested to involve the work effort, the fulfilment of the nutrient needs of the sheep and the feed waste. Mechanisation of the feeding can be very simple or include high degrees of mechanisation. Three different farms, representing different levels of mechanisation are presented. Descriptions and analysis of the feeding systems including mechanisation, work effort and estimations of the feed waste are made on each farm. The herd with the most advanced feed mechanisation included a rolling feed band and mix wagon. The other farms were partly or little mechanised including ad lib feeding from big bales and manual distribution of hay and concentrates. The most mechanised farm was able to feed a large herd of sheep at a low work effort and little feed waste. However, the investment in machinery has to be weighed against the improved efficiency.

Varför ger inte fler svenska företag ut konvertibler?

The purpose of this thesis is to increase the understanding of why convertible bonds are a relatively uncommon financing method in Sweden. The aim is to find the explanatory factors for this situation. The analysis is based on interviews conducted from three perspectives; the investor?s, the adviser?s and the issuer?s. The main finding is that the Swedish financial market has characteristics that inhibit the development of a convertible bond market in Sweden, leading to the fact that most convertible bonds are issued under special circumstances.

Önskvärda positioner och skamstrategier : En kvalitativ intervjuanalys om hur kön görs och används i BDSM

In this essay, I interview six users from the website darkside.se, which is an internet community for BDSM practitioners. I?m using Judith Butlers theorys about performativity and heteronormativity, Gayle Rubins model the charmed circle; of which sexualities is regarded as approved and which is not, and Fanny Ambjörnssons explenation of queer theory. With the interviews as my material, I investigate how sex and gender is produced and used in BDSM and at Darkside. I discuss trans, heteronormativity, sexism and hierarchies of power related to sex and gender, in BDSM and at Darkside.

Bokprat för barn: ur ett förmedlarperspektiv

The aim of this masters thesis is to explore a mediators practical procedure during booktalks. The main question is: Why do librarians have booktalks and what does it mean to have booktalks for children? In order to answer our main question, we have used these three questions: - Why do our interviewed librarians have booktalks? - How do our informants prepare their booktalks and what do their presentations look like? - Do our informants have any follow-ups of their booktalks and if so, how do they do that? To limit the thesis, we have concentrated our study to Swedish public libraries and to children in the elementary school. We have conducted qualitative interviews with three librarians who do booktalks for children as a part of their job. The thesis theoretical basis is Aidan Chambers theory about the Reading Circle and especially the parts enabling adult and selection.

Comparison of real-time PCR assays for screening of meticillin-resistant Staphylococcus aureus

Staphylococcus aureus belongs to the normal flora. Many healthy people are colonized by the bacterium mainly in the nose but also on the skin and on other mucous membranes without showing symptoms. After damage to the skin, the bacterium can enter the wound and cause infections. Methicillin-resistant S. aureus (MRSA) is resistant to b-lactam antibiotics such as penicillin and methicillin.

Koloniseringen av Thailand : En postkolonial studie om svenska backpackers i Thailand

Det är en betydande andel svenska resenärer som idag reser till och runt i Thailand som ?backpackers?. Mitt syfte är att ta reda på om den svenska backpackerdominansen i Thailand skulle kunna betecknas som en kolonisering. Material och metod består av intervjuer och deltagande observation med sju informanter, samt fältundersökningar som bedrivits i Thailand. Slutresultatet tolkas med hjälp av den postkoloniala teoribildningen.

Med passare och snöre : att planlägga ett hus under järnåldern

This essay concerns the interpretation of house plans in three-aisled longhouses and hall-buildings, in Scandinavia during Iron Age. Full scale house reconstructions need a better basis of interpretations to understand the patterns of roof supporting post holes in excavated house plans. This has led to the thesis, presented here, that the layout of house plans during Iron Age is based on geometrical proportions. In order to prove this, geometric house plans were applied and compared with excavated house plans in 11different case studies, including the fortification of Fyrkat Denmark. It was found that all house plans in the case studies indicate, that the geometrical proportions 1:2, 1:3 and 2:3 of a given circle must have been used for the basic layout of the roof supporting post holes, using a compass and a straightedge.

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