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9046 Uppsatser om Real time PCR SYBR-green - Sida 3 av 604

Optimering av realtids-PCR för identifiering och kvantifiering av Humant T-lymfotropt virus

Human T-lymfotropt virus (HTLV) är ett C-typ onkovirus som tillhör familjen Retroviridae som huvudsakligen angriper T-lymfocyter och orsakar leukemi och andra autoimmuna sjukdomar. I den nuvarande kliniska diagnostiken används Enzyme-linked immunosorbent assay och ibland vid screeningsmetoden uppträder ospecifika reaktioner. På senare tid har flera metoder baserade på real-tids PCR utvecklats för att bestämma halten av virala genom i patienter. Syftet med denna studie var att sätta upp och optimera en realtids-PCR för detektion av humant T-lymfotropt virus typ-1 och 2. Inför optimering av realtids-PCR extraherades DNA från MT-4 och MO-T cellinjer.  Under optimering av realtids-PCR användes SYBR Green och smältpunktsanalys där flera komponenter bl.a.

Värdet av att bygga grönt för kommunala bostadsbolag

AbstractThe real estate sector is today responsible for 40 per cent of the total amount of energy consumption within the members of the EU. All over the world there are collaborations within research and projects to find ways of reducing the amount of energy consumed by the real estate sector. In Sweden, a directive from the European Union has posed demands on authorities as well as on the private sector to reduce the amount of energy consumed by new building constructions. Together with the directive from the EU there are also new requirements and demands from Boverket for the real estate sector.  These requirements mostly concern the energy consumption of kilowatt-hour per square metre and that the consumed energy also should be renewable.  The aim of this study is to explain what kind of value municipalities experience in using certifications and environmental investments for new and existing buildings. Within the chapter theory, essential areas are described such as; environmental certification systems, economical viability, proceeds connected to building green, difficulties with building green, the performance of green buildings when affected by the behaviour of tenants and customer value.  The following chapter describes the methodology with which we have conducted this study.

Grönstrukturen i Örnsköldsvik : utvecklingen av det gröna Örnsköldsvik, ett led i skapandet av en grönplan

During the autumn of 2008 I obtained office experience by working at the local government of Örnsköldsvik. I started working on the district green plan of the green structure and that led to the work of this exam paper. The purpose of this exam paper has been to define some standpoints for the green structure of the local government district of Örnsköldsvik. I have used both a larger and a smaller scale to obtain a wide a perspective as possible of the green structure. The larger scale gives an overall view of the whole municipality while the smaller scale is found in the study of the two local areas Centrum and Domsjö. For the local government planning to be able to obtain an agreeable level of verdure to the residents it is necessary that the green structure is brought up on the agenda.

Är Grönt Skönt?: En studie om hur effekten av grön marknadsföring påverkas av förekomsten av miljöargument inom produktkategorin

Al Gore may have helped put global warming on the agenda. However, it is not a new subject. Neither is so called green marketing a new phenomenon within branding and communication. As more and more companies establish environmental strategies there exist beliefs that the environmental focus is becoming a triviality, which is the reason for this study. Implementation of words such as ?environmentally friendly?, ?green? and ?global warming? is becoming more and more widespread and frequently used within marketing communication.

Stereoseende i realtid

In this thesis, two real-time stereo methods have been implemented and evaluated. The first one is based on blockmatching and the second one is based on local phase. The goal was to be able to run the algorithms at real-time and examine which one is best. The blockmatching method performed better than the phase based method, both in speed and accuracy. SIMD operations (Single Instruction Multiple Data) have been used in the processor giving a speed boost by a factor of two..

Frekvens och spanningsskalning i en inbyggd mikroprocessor; for att mojliggora implementationen av schemalaggning med stod for dynamisk frekvens- och spanningsreglering for energihantering.

Minimizing power consumption is a critical part of many embedded design projects. Thechallenge is to limit the power consumption of the system and at the same time providesatisfactory service to the user. With regards to microprocessors, the main way of limiting powerconsumption during program execution is to adjust the frequency and the voltage at which theprocessor operates.To enable the implementation of real-time scheduling with support for dynamic voltage andfrequency scaling, an exhaustive study of the Freescale i.MX31 embedded microprocessor isperformed. Specifically, the power consumption relationship to all relevant frequency andvoltage settings is investigated. In addition, to enable frequency and voltage scaling in a real-timeenvironment, a complete timing analysis of proposed voltage and frequency scaling methods isperformed and their suitability for use in real-time systems is evaluated.To integrate voltage and frequency scaling capability into OSE RTOS, a software module hasbeen developed for this operating system.

Aspect Analyzer: Ett verktyg för automatiserad exekveringstidsanalys av komponenter och aspekter

The increasing complexity in the development of a configurable real-time system has emerged new principles of software techniques, such as aspect-oriented software development and component-based software development. These techniques allow encapsulation of the system's crosscutting concerns and increase the modularity of the software. The properties of a component that influences the systems performance or semantics are specified separately in entities called aspects, while basic functionality of the property still remains in the component. When building a real-time system, different sets of configurations of aspects and components can be combined, resulting in different configurations of the system. The temporal behavior of the system changes and a way to ensure the predictability of the system is needed.

Problematik vid analys av fastighetsbolag efter införandet av IFRS

The study describes the problems with analysis of real estate companies after the introduction of IFRS. With the introduction of IFRS (2005) and specially IAS 40 the real estate companies get the ability to appreciate its real estates to real value. With the difference in the accounting, grows the question if it has become some differences for analysts with analysis and valuation of real estate companies. We also look into how well this new standard effect the real estate companies from the view of the accounting quality and its characteristics..

Deriving ECA-rules from timed-automata specifications.

Real-time systems are required to answer to external stimuli within a specified time-period. For this to be possible, the systems behaviour must be predictable. The use of active databases in real-time systems introduces unpredictability in the system, e.g. due to their use of active rules. The behaviour in active databases is usually specified in ECA-rules.

Grönare IT med hjälp av balanserat styrkort

The concept of green IT is a hot topic in the industry right now. The requirements forenvironmental friendliness are becoming increasingly common. There is a lack of knowledge andunderstanding of what green IT means and how it can be achieved. Our study shows that the useof ICT can never become entirely green, but a lot can be done to become greener. We haveproduced a balanced scorecard which in theory leads to better governance, increased knowledgeand a common picture of what the work of greening IT means for employees and theorganization.

Buntning av grot med lastbilsmonterad utrustning :

During the transportation of logging residues (LR) the material´s low density is a problem that results in low use of pay-load capacity. Comminution or compression of LR are possible methods to increase pay-loads. In the present thesis studied a bundling machine which was mounted on a truck chassis, and the subsequent transport and crushing. The truck bundler created bundles that were larger and longer than other bundling equipment on the market. The objectives were to determine the total time consumption and its allocation over work element and machinery, to measure dry matter (DM) content of green and brown LR-bundles.

Going Real-Time in no time? : ? en kvantitativ och kvalitativ studie i hur Sveriges största annonsörer använder sociala medier och Real-Time Marketing.

Background: Our daily lives and personal interactions are increasingly featuring social media and social networks in particular. Topics regarding social media, its impact on society at large ? both on corporate as well as individual behavior ? has been the focus of many research papers. Personal use of social networks are regularly being mapped out by researchers aiming at clarifying concepts such as engagement, trust and interactivity, and mapped by companies seeking to maximize return on investment concerning their marketing efforts. The aim of our study is (i) to map how companies and/or organizations operating on the Swedish market use their company?s pages on the social media as a way of communicating with their followers and audience, as well as (ii) identifying whether ?Real-Time Marketing?, in our own definition, is used or not.Methods: The research methods used included both quantitative and qualitative content analysis.

Mäklaryrket ur ett jämställdhetsperspektiv

The Profession of Real Estate Agents has by tradition been a male profession. However during later years the profession has changed. It is now many young women that are pursuing a career as a Real Estate Agent.In the light of this background we have researched how Real Estate Agents employed by Fastighetsbyrån and Svensk Fastighetsförmedling perceive their profession from an equality perspective.The investigation has been limited to include the counties of Värmland and Närke and have been conducted by interviewing Real Estate Agents of different age and sex and the managers of the firms.The study show that the Real Estate Agents perceive their profession in a positive way and that the work is divided equal. However it emerged that there were a division regarding different real estate types in certain agencies. The Real Estate Agents believe that the gender of the manager is of no importance.

Implementation av en renderare för Agency9

The visual complexity in real-time applications, like computer games, is increasing with customer demands on vissual apperance. This trend is supported by even better graphics cards from the hardvare industry. This must be taken into consideration when the architecture of a new graphics engine is to be designed. The engines renderer is responsible of generating a picture of scene data. The renderer must cope with increasingly more complex scenes, and must be able to render them depending on available hardvare capasity.

GRÖN FLAGG : Miljöarbete i förskolan

The aim with my work is to get a deeper knowledge about the preschool's views of environmental work. I also want to examine how the preschool teachers along with the children work with the project Green Flag in the preschool. I have used structured interview with two teachers in a preschool that works with Green Flag. Green Flag is an environment certification and is an evidence on that one works daily with a document aimed environmental work along with the children. I have found out that each year they work with different themes within Green Flag.

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