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2 Uppsatser om Phenotyping - Sida 1 av 1

Phenotyping of Bali cattle and interviewing farmers in Indonesia - a minor field study

Among the Indigenous cattle breeds in Indonesia, the Bali cattle is the most preferred by small farmers. The Bali cattle is a beef breed, and is considered to have several advantageous characteristics and to be well adapted to the country?s harsh environmental tropical conditions with drought and rain. The main aim of this project was to phenotype of Bali cattle from different locations in order to identify and document diversity within the Bali cattle breed based on their observable characteristics, to compare cattle from different areas and production systems to see the effect of management on phenotype. In this study, 107 cows and bulls of the breed Bali cattle, from Kalimantan, Sumatra, Lombok and Bali were phenotyped.

Comparison of Different Electrophoretic Methods for Haptoglobin Phenotyping and an Investigation in Patients with Abdominal Aortic Aneurysm

Haptoglobin is an acute phase protein with important biological role because of its capacity to bind to haemoglobin. Haptoglobin exists in three major genetic polymorphism types: Hp1-1, Hp2-1 and Hp2-2, the distribution of which has been associated with abdominal aortic aneurysm (AAA), an asymptomatic aortic disease common among men older than 65 years.    Five different electrophoretic methods were tested according to their ability to separate the haptoglobin phenotypes. The detection was based on a produced hemolysate of blood in which haemoglobin binds to haptoglobin thereby forming a complex that can be detected by specific haemoglobin staining using TMB-dihydrochloride and hydro peroxide as substrate resulting in an azure-green color of the bands. Samples from 15 patients who had suffered surgery for not broken AAA, that is more than5.0 cmaortic diameter, and 15 samples from matched controls were analyzed.    Among the five tested electrophoretic methods best migration and separation was seen on the pre-cast agarosgel Hydragel HR on the instrument Hydrasys. The other four methods gave less successful results.