Aim: The aim of this paper was to investigate whether scientific evidence exists to improve, of students? perceived, insufficient infection control in isolation facilities in companion animal hospitals in Sweden. Material and methods: A question was emailed to the students in the last year of the animal nursing program at SLU in the spring 2012 to get their view of perceived insufficient infection control. A literature study was thereafter preformed using the search engine Web of Knowledge. Results: The students reported several perceived shortcomings which were divided into five categories: basal hygiene, isolation routines, cleaning and disinfection, patient flow and other. Studies supporting that the shortcomings perceived by the students also exist in human health care and to some extent in animal clinics and hospitals were found. Furthermore, scientific evidence that improved hand hygiene, glove procedures and cleaning and disinfection reduces the risk of nosocomial infections in humans was reported. Conclusion: There is not a lot of research performed in the area of infection control in animal nursing.

Escherichia (E) coli is one of the most common udder Pathogens associated with acute clinical mastitis in Swedish dairy cows. The aim of this study is to review some of the existing literature on E coli mastitis from a Swedish perspective. The bacteria, its most important characteristics, and diagnostics under field conditions is covered, as well as what happens during infection, how E coli affects the cow and which consequences this have. Clinical symptoms associated with E coli mastitis are described and information on when these cases of mastitis usually occurs is given. Risk factors in the cow?s enviroment are described as well as the importance of the cow?s immune defence.

The aim of the study was to investigate if some selected recipes could be hazardous for man; that is if pathogenic bacteria in raw ingredients could survive the cooking process. The study included three known Pathogens, Listeria monocytogenes, Salmonella Typhimurium and Campylobacter jejuni. A known number of the bacterium chosen was added to the raw product. The dish was prepared according to the recipe and then a bacteriological investigation concerning the added pathogenic bacterium was performed. The results showed that all recipes tested could be hazardous; that is the added pathogen was still living after the cooking process..

Eight healthy 3-day-old foals were given repeated injections of trimethoprim-sulfadiazine intravenously for 3 days, at a dosage of 15 mg/kg bodyweight (2,5 mg trimethoprim and 12,5 mg sulfadiazine). Blood samples were collected prior to each administration and for the following 24 hours after last administration. Serum concentrations of trimethoprim and sulfadiazine were measured and the pharmacokinetics for the substances were studied. The elimination half time (t½) and clearance (ClB) for trimethoprim and sulfadiazine for the foals did not diverge from adult horses. According to these data it is possible to assume that the same dose interval and dose can be used for foals as for adult horses.

Eight healthy 3-day-old foals were given repeated injections of trimethoprim-sulfadiazine intravenously for 3 days, at a dosage of 15 mg/kg bodyweight (2,5 mg trimethoprim and 12,5 mg sulfadiazine). Blood samples were collected prior to each administration and for the following 24 hours after last administration. Serum concentrations of trimethoprim and sulfadiazine were measured and the pharmacokinetics for the substances were studied. The elimination half time (t½) and clearance (ClB) for trimethoprim and sulfadiazine for the foals did not diverge from adult horses. According to these data it is possible to assume that the same dose interval and dose can be used for foals as for adult horses.

Eight healthy 3-day-old foals were given repeated injections of trimethoprim-sulfadiazine intravenously for 3 days, at a dosage of 15 mg/kg bodyweight (2,5 mg trimethoprim and 12,5 mg sulfadiazine). Blood samples were collected prior to each administration and for the following 24 hours after last administration. Serum concentrations of trimethoprim and sulfadiazine were measured and the pharmacokinetics for the substances were studied. The elimination half time (t½) and clearance (ClB) for trimethoprim and sulfadiazine for the foals did not diverge from adult horses. According to these data it is possible to assume that the same dose interval and dose can be used for foals as for adult horses.

The aim of this report is to create an easily accessible guide to basic hygiene, cleaning and disinfection within the small animal practice. It is based on a literature study, using mainly research from the public health sector, due to the lack of relevant scientific reports within the veterinary field. To prevent the spread of infectious diseases it is important that the staff understands the importance of basic hygiene. Hand hygiene and disinfection and cleaning with water and detergent combined are the most important routines to prevent spreading of disease. In conclusion the differences between the veterinary and the public health sector are small, but some basic differences exist due to the nature of the patient?s needs.

Every year about 210 000 tones dry weight of sewage sludge is produced in Sweden. Most of the sludge is applied to agricultural land, plantsoil and landfill cover. Sewage sludge contains essential nutrients such as nitrogen and phosphorus. Nitrogen is limiting growth on solid ground in Sweden and can therefore be used as fertilizer to increase productivity. In order that the sludge can be spread to forest land it requires first a conversion of sludge to pellets, to make it more manageable.

Fusarium graminearum is one of the most important Pathogens on cereals and causes major crop losses around the world. The most cultivated cereal in Sweden is winter wheat. F. graminearum produces both sexual and asexual spores for dispersal. Multiple factors affect the production of these spores.

Today, there are more than 12 000-14 000 farms worldwide using an automatic milking system (AMS). Studies have shown that transition to AMS affects the udder health of cows adversely, recognized primarily as elevated somatic cell counts. There are several reasons for this, for example the greater variation in milking frequency seen in AMS. On one hand, short milking intervals may lead to bad teat condition of the cows due to the stress the milking procedure exposes the teats to. Too long milking intervals, on the other hand, seem to predispose for milk leakage, which is, in turn, a risk factor for the development of mastitis.

The purpose of this study was to investigate the occurrence of Verotoxin-encoding bacteriophages in mussels, cultured downstream the sewage treatment plant in Lysekil.Mussels were collected in three growing areas from April 2008 to March 2009. Real-time PCR was performed for detection of vtx1 and vtx2 genes and enrichment of bacteriophages on non Verotoxin-producing Escherichia coli O157: H7 was carried out. All samples in real-time PCR analysis were negative; no presence of Verotoxin-encoding phages was shown. No plaque was formed on blood agar base plates, indicating that no bacteriophages had been taken up by E. coli bacteriaThe levels of Verotoxin-encoding phages and E.coli outside the sewage treatment plant in Lysekil were not high enough to be able to form VTEC in mussels, indicating that the faecal contamination was low.

Two species of bees in the genus Apis, real honey bees, has long been of interest for man. These two are the European honey bee, Apis mellifera, and the Asian honey bee, Apis cerana. In Vietnam, beekeeping is of great importance, both with A.cerana and A.mellifera. The aim of this project was to investigate if the introduction of the European honey bee in Asia has affected the Asian honey bee, and whether different Pathogens from A.mellifera have been transferred to A.cerana. Totally 40 samples, 20 from every species, were analysed for 8 different viruses.

Drinking water is produced from raw water and is either from groundwater or surface water. This thesis aims to find out if the cleaning process of raw water is sufficiently effective. This is important because consumers are otherwise at risk of waterborne infection caused by Pathogens. There are three groups of Pathogens; bacteria, virus and parasite. These have different characteristics which mean that they require different water treatment to be separated.

Drinking water is produced from raw water and is either from groundwater or surface water. This thesis aims to find out if the cleaning process of raw water is sufficiently effective. This is important because consumers are otherwise at risk of waterborne infection caused by Pathogens. There are three groups of Pathogens; bacteria, virus and parasite. These have different characteristics which mean that they require different water treatment to be separated.

Background: As a part of a national health control program, Statens VeterinärmedicinskaAnstalt performs diagnostics to screen flocks for certain Pathogens causing high mortality,morbidity and/or serious economical losses. There are several viruses in the programincluding IBDV (infectious bursal disease virus), IBV (infectious bronchitis virus) and NDV(Newcastle disease virus). Method: 96 serum samples were collected from different poultryflocks in Sweden and analyzed by ELISA, which are currently used in the health controlprogram as well as by a commercial prototype of a multiplex immunoassay manufactured byLuminex Corp., which is currently under evaluation at the United States Department ofAgriculture USDA. This 4-plex assay detects antibodies for the three above-mentionedviruses as well as antibodies of avian reovirus. In the context of this study the ELISAs run inroutine diagnostics as well as a REO ELISA were used as the standard for comparison.Result: The antibody concentration in serum from vaccinated chickens was high while theantibody concentration level in serum from not vaccinated chickens was low.