Crypreservation of Oocytes is recently being considered to be a valid choice in infertility treatments.Low survival and fertilization rates due to inefficient slow freeze protocols have been the outcome ofmany previous studies done in the field. However, introduction of the vitrification technique and itsapplication in reproductive medicine and to some extent new improved slow freeze protocols haveshown that Oocytes can be cryopreserved with successful outcome.In this project the survival rate of Oocytes after vitrification with MediCult Vitrification andWarming Media has been studied. Also, a comparison of the carriers Cryoloop (an open system) andCryopette (a closed system) has been performed.A total of 43 Oocytes were vitrified and warmed according to MediCult's protocol, of which 21Oocytes with Cryoloop and 22 with Cryopette. The cells were post-thaw incubated in a physiologicalenvironment for 24h. During that time the morphology and viability were observed and noted after 2h,over night and after 24h.

Involuntary childlessness is a big problem around the world. One way to solve the problem is to receive Oocytes or semen from a donor. In Sweden, semen donation has been regulated by law since 1985 and oocyte donation since 2003. The aim of this study was to investigate what motivations women and men in Sweden have to donate gametes, if they feel any ambivalence to donate, and to compare if there is any difference in motivation and ambivalence between women and men. Motivation was measured with eight statements based on previous results and clinical experience.

Fertility is of central interest in the dairy production but has during the last decades declined. Increased milk yield has resulted in high pressure on the metabolism of the dairy cows that are supposed to manage the transition from dry cows to lactating cows within a few weeks around the parturition. Much indicate that metabolism and fertility are closely linked, with insulin playing a substantial part. There are many studies suggesting that the main part of gestation loss can be found during the early embryo development, a period which can be studied in vitro. The aim of this study was to test the effect of insulin during maturation in vitro and to evaluate two different fluorescent stainings on Oocytes and embryos; a nuclear stain and a staining of apoptotic cells through the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-method. Bovine cumulus-oocyte-complexes (n=991) were aspirated from abattoir-derived ovaries.

Ovarian stimulation in assisted reproduction often leads to the production of a high number of Oocytes. After fertilization of these Oocytes, the resulting embryos can be cryopreserved for later use. Vitrification is a recently introduced method for cryostoring embryos, showing high survival rates for both cleavage stage embryos and blastocysts. Characteristic of vitrification are high concentrations of cryoprotectants and ultra fast freezing which makes the material glassily. A major concern with vitrification has been the direct contact of the cryo-solutions with liquid nitrogen.

A number of chemical agents, and their degradation products, have been recognized as having estrogenic activity on animals. On fish they interfere with endogenous estrogen and in many cases mimic its activity, thus increase estrogenic reponse, with elevated levels of vitellogenin. Hence they are called Endocrine Disrupting Chemicals, EDCs. Vitellogenin levels is often used as a biomarker of exposure to estrogenic pollutants. Wastewater from sewage treatment works contains chemicals known as EDCs, and there are evidence of roach (Rutilus rutilus) living in recipients of such wastewater having traits of both gender, i.e. Oocytes in male gonads (intersex) on histological examination. This study was done on roach living in Lake Funbosjön Uppland, Sweden.