Biogas is a renewable energy produced through anaerobic digestion, which means that organic matter is degraded by Microorganisms under anaerobic conditions. The produced biogas can then be used for cogeneration, electricity, heat or upgraded to vehicle gas. Eskilstuna Energy & Environment AB has four digesters at Ekeby water sewage treatment plant, Eskilstuna. One of the biogas reactor (RK4) is equipped with a jet-mixing system while the remaining digester uses top-mixing. The mixing system is an important parameter to achieve optimum operating conditions for the process regarding gas production and degree of digestion.

The aim of this paper was to explain protein degradation in silage and protein quality and biogenic amines in silage and their effects on the health of the dairy cows. After harvest-ing, the plant proteases degrade the proteins in the plant. The protein degrades to different fractions with different digestibilites in the rumen. Fraction A contains non- protein nitro-gen which is peptides, free amino acids and amines. True protein is in the fraction B and is further divided into three subfractions depending on their solubility.

In this thesis the methane gas potential of three different substrates, two algaes Saccharina latissima and Laminaria digitata and biosludge mixed with paper fiber was studied. This was done by batch experiments in a laboratory environment to examine the gas production and composition of the produced gas.Biogas production is a complex anaerobic digestion process in which various Microorganisms decompose the substrate in steps and at the end produce biogas and a residue. Many factors affect the production of gas, for example the substrate content, temperature and pH in the digester.The analysis of methane potential were divided into two experiments. In experiment 1 substrates were digested along with inoculum from Växjö waste water treatment plant in a temperature of about 37 ?C.

One of the main objectives of all food production is to produce foods that can safely beconsumed without fear of being sick. Despite this hundreds of people are affected in Sweden offood poisoning almost every day. Most cases are caused by a small group of so-called pathogenicMicroorganisms. The most frequently affected are the small children, the elderly, persons withreduced resistance and pregnant. The reasons behind the food poisoning are often ignorance andlack of food hygiene.

In the production of pulp, paper and cardboard, a large amount of water is used daily. The water has to be purified in the internal purifying plant before it reaches the receiving body of water. In the biological purifying stage at the Stora Enso Skoghall mill, an aerated basin is used where the Microorganisms, using oxygen, oxidize the organic material to carbon dioxide. The air is pumped from the bottom of the basin and the oxygen can then be transported from the air bubbles to the water through diffusion. The problem with aeration of waste water from the forest industry is that wood residues, such as fatty acids, are making the transport of oxygen in water more difficult.

Wastewater contains biodegradable materials, which are often measured as BOD7.Trickling filters are sometimes used as a purification step for BOD-reduction. Atrickling filter is a tower, which is filled with a carrier material. Microorganisms growon the carrier material in the form of a biofilm. This biofilm consists mostly of bacteriathat purify water from BOD.In Älgarås? wastewater treatment plant the water is purified by a trickling filter.However the treatment plant has difficulties reaching the emission requirements forBOD.

Identification of fungi is based on macroscopic observations of morphology and microscopic characteristics. These conventional methods are time-consuming and requires expert knowledge. For the past years Matrix-assisted laser desorption ionization-time of flight mass spectrometry has been used for routine bacterial identification in clinical laboratories but not yet in the same extension for fungi. In this study three preanalytic preparation methods for fungi were evaluated in order to shorten the processing time in routine laboratory performance.Clinically relevant strains (n=18) of molds and dermatophytes were cultivated on agar plates and prepared according to the different preparation methods for protein extraction. Each strain was analyzed in quadruplicate by the MALDI Biotyper and the database Filamentous Fungi Library 1.0.The results showed that the genus and species identification rates of the least time-consuming direct extraction method were 33% and 11% respectively.

ABSTRACTChlamydia and Chlamydophila are species of a family of bacteria (Chlamydiaceae) that cause a range of diseases, including conjunctivitis, in a variety of animals including pigs and humans. Some species cause zoonotic disease. This study examined the occurrence of Chlamydiaceae in samples taken from the lower eyelid conjunctiva in pigs with and without conjunctivitis. The study was initiated based on a herd investigation aimed to examine the experienced increase of conjunctivitis in some finisher herds. This study was conducted as a case-control study, by collecting samples from 62 case pigs (16-26 weeks old) and an equal number of controls, from three herds in Uppland, Sweden.

In the production of pulp, paper and cardboard, a large amount of water is used daily. The water has to be purified in the internal purifying plant before it reaches the receiving body of water. In the biological purifying stage at the Stora Enso Skoghall mill, an aerated basin is used where the Microorganisms, using oxygen, oxidize the organic material to carbon dioxide. The air is pumped from the bottom of the basin and the oxygen can then be transported from the air bubbles to the water through diffusion. The problem with aeration of waste water from the forest industry is that wood residues, such as fatty acids, are making the transport of oxygen in water more difficult.

Denna rapport är en sammanställning av de 100 undersökta enskilda brunnarna i Landskrona kommun vid årsskiftet 05/06. Det utfördes analyser på mikrobiologisk-, nitrat-, nitrit- och även kemiska bekämpningsmedelspåverkan. Syftet med denna rapport är att bearbeta och presentera analysresultaten av de 100 enskilda brunnarna i Landskrona kommun och försöka dra slutsatser kring dessa. Alla analysresultaten sammanställdes i tabeller och så småningom kundes vissa slutsatser dras kring varför resultatet såg ut som det gjorde. Resultatet visade att endast åtta av de 100 brunnarna var otjänliga gällande mikrobiologisk påverkan.

To establish good horse welfare there are several factors that need to be considered. One important factor is the environment in which the horses are kept. In Sweden horses are stabled during long periods of time in the cold season and consequently the stable environment is even more important. The most essential environmental factors in a horse stable are ambient temperature, relative humidity (RH) and concentrations of contaminants in the stable air. The air in a horse stable contains dust, noxious gases, moulds and Microorganisms which all can contribute to developing respiratory diseases in horses.

The codling moth, Cydia pomonella is a common pest in apple orchards and can be found worldwide. Feeding by codling moth larvae can cause a substantial decrease in apple yield. Control methods comprise for example the use of pesticides, viruses and pheromones. As a sustainable control method, pheromones have been successfully applied to disrupt moth mating behaviour. Other behaviours like foraging, search for mating sites or host finding could be potential targets for additional odour-mediated behavioural manipulation but need further investigations of the underlying odour stimuli and behavioural plasticity. Many insects are attracted to the smell of fermenting fruit and associated Microorganisms.

Iron deficiency is one of the most frequent micronutrient deficiencies around the world. Low iron bioavailability simultaneously with a high iron requirement is a high risk factor for developing iron deficiency. Probiotics are Microorganisms that confer a health benefit on the host. Prebiotics are oligosaccharides which provide a health benefit on the host due to a positive modulation of the microflora in the gut. The aim of this paper is to evaluate if there is a connection between pro-and/or prebiotics and iron bioavailability.

Abstract Cavities used to be a very common problem but over time humans have learned to take care of their teeth. However, there are still many who have problems. This problem is due to Microorganisms and in particular the various streptococci and lactobacilli producing acids which loosens up the enamel on teeth. These bacteria can be found at various places in the mouth and saliva. Besides being a discomfort it can be a major problems and the damaged tooth needs to be removed or make a dental filling. The aim of the study was to see how intake of probiotic tablets containing Lactobacillus reuteri PTA ATCC 5289 and Lactobacillus reuteri DSM 17938 affects the levels of oral Streptococcus mutans and lactobacilli over a three-week period.

The aim of the project was to develop a method to investigate differences in adhesion of Microorganisms to materials that contains lignocellulose. The method was tested on a gram-positive (Micrococcus lutea) and one gram-negative (E-coliJM109) bacteria.The study was begun by cultivation of the two Microorganisms. The cultivation was done to calculate the generation times of the bacteria and to obtain growth curves. Cells from these cultivations were also frozen (-70ºC) and later used for inoculation.At STFI-Packforsk AB the total charge on the mass was measured and later a conductivity titration on the mass was executed as well, all to find out more about the different properties of the mass. Properties that in a later part of this study could possibly be linked to the adhesion of cells to the pulp.