Bakgrund: Resistenta bakterier (ESBL och MRSA) o?kar runtom i Europa och anses vara ett hot mot ma?nniskors ha?lsa. Det finns en kunskapslucka bland sjuksko?terskor vad ga?ller smittva?gar fo?r dessa bakterier och hur omva?rdnad bo?r ges till smittade patienter. Syfte: Att underso?ka resistenta bakteriers smittva?gar och vilka a?tga?rder i omva?rdnaden av patienter som kan bryta dessa smittva?gar.

SammanfattningMykotoxiner är sekundära metaboliter som kan bildas av vissa mögelsvampar. Idisslare har generellt ett högre skydd mot mykotoxiner än vad enkelmagade djur har då våmmens mikroorganismer kan fungera som ett extra skydd mot mykotoxikos. Trichotecenerna deoxynivalenol och T-2 toxin bryts ner till de-epoxymetaboliter i våmmen genom att dess toxiska epoxyring spjälkas bort. Deoxynivalenol metaboliseras helt eller delvis av våmmens mikroorganismer, medan T-2 toxinets nedbrytningskapacitet inte är lika hög. Aflatoxin B1 har en låg nedbrytbarhet, och inga metaboliter har kunnat detekteras i våmmen.

Hjärt-kärlsjukdomar är den vanligaste dödsorsaken i Sverige. 41 % av kvinnorna och 39% av männen avled till följd av hjärt-kärlsjukdomar år 2011. Då ambulanspersonalen ofta har begränsad tid på sig att etablera kontakt och skapa sig en helhetsbild över patientens tillstånd, vilket är viktigt för att säkerställa en god behandling, kan kravet på effektivitet bli en stressfaktor. För att minska stressen kan det vara bra att utgå från en strukturerad bedömning av patientens tillstånd och där en god anamnes är ett viktigt redskap. Det finns utarbetade instrument för detta,däribland anamnestagande enligt Advanced Medical Life Support®-konceptet(AMLS).

Powdery mildew is a serious problem in both field and greenhouse cucumber production and can cause serious infection and radically reduced yields. Today, powdery mildew is the main disease in greenhouse produced cucumber. Disease control should preferably be both efficient and environmentally friendly, which is not easy to achieve at all times. Alternatives to chemical control of powdery mildews are biological control, physical control, non-fungicide control and cultivation of resistant or tolerant varieties. Biological control can be described as the suppression of damaging activities inflicted of a harmful organism by one or more other organisms, which are called antagonists or natural enemies. In Sweden, there are currently no registered biological control products available to control cucumber powdery mildew. The aim of this study was to investigate the effect of two biological control products in order to evaluate their ability to prevent or reduce powdery mildew infection to an acceptable level in greenhouse produced cucumber. In the experiments, different application regimes were also studied.

The price trend on rapeseed the latest years has led to an increasing interest to grow the crop among many farmers in Sweden. The price trend has resulted in more than a doubling of the oilseed area since the early 2000s. For many farmers it is an advantage to increase cultivation of rapeseed because of the high proportion of cereals in the crop rotation on many farms, but it is not just benefits. The risk of the fields being infected by clubroot increases within frequent rapeseed cultivation and it often leads to subtantial economic losses. Clubroot is caused by Plasmodiophora brassicae that is a soilborne pathogen in genus Protozoa and its resting spores can be found in the soil for up to 18 years.

The focus of this project was to characterize the occurrence of ESBL-­?producing bacteria in two irrigation water ponds in Southern Sweden. Samples were taken from two ponds nearby each other, from which analyses were made based on levels of community and individual isolates. Community samples were used for attempts for characterization of resistance patterns in irrigation water using the Omnilog PM-­?plate system. The growth of community microbiota in the presence of the following antibiotic substances was assessed; amikacin, gentamicin, kanamycin, penicillin G, ampicillin, piperacillin, trimethoprim, potassium tellurite and ciprofloxacin. Isolates were pure cultured using semi-­?selective media: LB, Rainbow Agar, mEnterococcus, 0.1 TSA and VRBD. A total of 177 isolates were isolated randomly. Each isolate was identified using the Omnilog GENIII-­?system of biochemical characterization. Further, the isolates were tested on Brilliance ESBL-­?agar for their ability to grow. Out of the total number of isolates, 30 were selected, preferably Enterobacteriaceae or Pseudomonas spp., based on their high similarity to library strain during the identification in the Omnilog-­?system. These were further characterized using Etest-­?sticks. Three selected strains were picked for further analyses using exogenous isolation for attempts to simulate horizontal gene transfer, and Omnilog PM-­?panels containing various antibiotics of interest. Selections of samples were chosen for molecular tests targeting plasmid-­?borne resistance genes CTX-­?M1, CTX-­?M2 and SHV. The method for assessing community-­?resistance patterns showed ambiguous data that was difficult to interpret; further optimization is recommended. For all tested PM-­?plates, generally greatest differences between substances were found among wells with the strongest concentration of antibiotic substance. A total of 175 isolates displayed ability to grow on ESBL-­?agar. Etests confirmed ability of isolates to resist many ?-­?lactam antibiotics and many were clinically resistant to certain substances. No plasmids were transferred during exogenous isolation. During molecular tests, a few matches were found for the CTX-­?M1 gene. Isolates analyzed with Omnilog PM-­?plates showed resistance to most compounds tested. The isolates showed greatest resistance toward penicillin G, ampicillin, amikacin, kanamycin and trimethoprim. In the study, it was established that resistance among bacteria was frequently occurring in the irrigation water, and ?-­?lactamase producing bacteria was very common. Extended studies are needed to assess the frequency-­? and occurrence of horizontal gene transfer (HGF) in this environment. Although some potential corresponding data is found, it is at this point not possible to determine whether the resistances are due to antibiotics used in veterinary medicine, human medicine or one additional reason is travel and trade. in irrigation water using the Omnilog PM-­?plate system. The growth of community microbiota in the presence of the following antibiotic substances was assessed; amikacin, gentamicin, kanamycin, penicillin G, ampicillin, piperacillin, trimethoprim, potassium tellurite and ciprofloxacin. Isolates were pure cultured using semi-­?selective media: LB, Rainbow Agar, mEnterococcus, 0.1 TSA and VRBD. A total of 177 isolates were isolated randomly. Each isolate was identified using the Omnilog GENIII-­?system of biochemical characterization. Further, the isolates were tested on Brilliance ESBL-­?agar for their ability to grow. Out of the total number of isolates, 30 were selected, preferably Enterobacteriaceae or Pseudomonas spp., based on their high similarity to library strain during the identification in the Omnilog-­?system. These were further characterized using Etest-­?sticks. Three selected strains were picked for further analyses using exogenous isolation for attempts to simulate horizontal gene transfer, and Omnilog PM-­?panels containing various antibiotics of interest. Selections of samples were chosen for molecular tests targeting plasmid-­?borne resistance genes CTX-­?M1, CTX-­?M2 and SHV. The method for assessing community-­?resistance patterns showed ambiguous data that was difficult to interpret; further optimization is recommended. For all tested PM-­?plates, generally greatest differences between substances were found among wells with the strongest concentration of antibiotic substance. A total of 175 isolates displayed ability to grow on ESBL-­?agar. Etests confirmed ability of isolates to resist many ?-­?lactam antibiotics and many were clinically resistant to certain substances. No plasmids were transferred during exogenous isolation. During molecular tests, a few matches were found for the CTX-­?M1 gene. Isolates analyzed with Omnilog PM-­?plates showed resistance to most compounds tested. The isolates showed greatest resistance toward penicillin G, ampicillin, amikacin, kanamycin and trimethoprim. In the study, it was established that resistance among bacteria was frequently occurring in the irrigation water, and ?-­?lactamase producing bacteria was very common. Extended studies are needed to assess the frequency-­? and occurrence of horizontal gene transfer (HGF) in this environment. Although some potential corresponding data is found, it is at this point not possible to determine whether the resistances are due to antibiotics used in veterinary medicine, human medicine or one additional reason is travel and trade..

Different methods for shelterwood cutting have been used in differ-ent parts of Europe since the middle ages. The use of shelter wood cutting in Norway spruce (Picea abies (L.) Karst.) forests in Sweden met a revival in the early 1990s as an alternative for the dominating clear-cutting system when silvicultural costs increased dramatically for the latter. Shelterwood cutting systems involve some possible problems but also a lot of positive effects. Among the problems are increased risk of wind throw and damage of the remaining shelter trees caused by the cutting operation. Positive effects are for example a valuable growth in the remaining trees and increased biological di-versity. This study is based on a total of 79 trees from 4 different treatments in a spruce shelterwood, cut in 1994, in the middle of Sweden (62,4º N).

Wounds on horses are common and sometimes difficult to treat. The wounds are often big and could be discovered too late for primary sutures, which have to be applied within 8 hours from time of accident, before bacteria has been able to infiltrate the surrounding tissue. The healing is often complicated by delayed healing and wound infection with consequences like phlegmone, fistulation and proud flesh formation.To avoid these consequences, the traditional practice has been to administer systemic antibiotics alone, systemic antibiotics in combination with topical antibiotics or topical antibiotics alone. Because few antibiotics can be used in horses, is it important to household with the few substances that are available. In veterinary medicine today, the increasing resistance amongst pathogenic microbes such as MRSA presents a growing problem.

Promoters are genetic elements that facilitate the transcription of a gene and they have been found in front of non-coding RNA (ncRNA) genes in different organ-isms, e.g. the model plant Arabidopsis thaliana. A similar element, DUSE, has been found in front of ncRNAs in the social amoeba Dictyostelium discoideum and a part of this project has been to analyze the function of this putative promoter element through cloning and expression studies. A construct to analyze the func-tion of DUSE was successfully designed and introduced into D. discoideum but full expression studies were not finished because of shortage of time.

This thesis is a queer masculinity reading of Sir Arthur Conan Doyle?s short story collection The Memoirs of Sherlock Holmes (1894). The analysis focuses on the dissonances, tensions and queerness that reside within the text itself. This has been done from my problem statement: How is Sherlock Holmes and John Watson?s sexuality and masculinity portrayed within the boundary of the text? What is being said, what is hidden, and what is dealt with silently? To reveal these queer parts this analysis has been focused around five themes: the late Victorian male, the Woman, countertypes and decadence, the homosocial sphere and sexuality.

Promoters are genetic elements that facilitate the transcription of a gene and they have been found in front of non-coding RNA (ncRNA) genes in different organ-isms, e.g. the model plant Arabidopsis thaliana. A similar element, DUSE, has been found in front of ncRNAs in the social amoeba Dictyostelium discoideum and a part of this project has been to analyze the function of this putative promoter element through cloning and expression studies. A construct to analyze the func-tion of DUSE was successfully designed and introduced into D. discoideum but full expression studies were not finished because of shortage of time.

Promoters are genetic elements that facilitate the transcription of a gene and they have been found in front of non-coding RNA (ncRNA) genes in different organ-isms, e.g. the model plant Arabidopsis thaliana. A similar element, DUSE, has been found in front of ncRNAs in the social amoeba Dictyostelium discoideum and a part of this project has been to analyze the function of this putative promoter element through cloning and expression studies. A construct to analyze the func-tion of DUSE was successfully designed and introduced into D. discoideum but full expression studies were not finished because of shortage of time.

During the period of occupation of Norway between the years 1940-1945 the National Lutheran Church took the responsibility of supporting the Norweigans in their ideological battle against the Nazis. This was a period named Holdningskampen in Norwegian history. One person to remember was Eivind Berggrav, bishop in Oslo diocese. He was the one responsible for the theological document of importance Kirkens Grunn that became important as a document against the Nazis and the small percentage of Christians that related to the naziinspired-church. Berggrav was also one of the founders of Den Midlertidlige Kirkeledelsen, the organisation responsible for the non-nazified churches.

Kvarstående betäckningsinducerad endometrit är ett stort problem inom hästnäringen och så även andra typer av endometrit. Detta är den främsta orsaken till infertilitet hos ston. Vissa ston är nämligen oförmögna att klara av den inflammatoriska reaktion som normalt följer av betäckning (för att eliminera sperma och patogener) eller så drabbas de lättare av kroniskt infektiös endometrit, oavsett betäckning. Det finns otaliga förslag till förklaringar till varför de inte kan hålla uterus fri från inflammation såsom defekta myometriekontraktioner, stoets reproduktionshistoria eller anatomiska aspekter (t.ex. pneumovagina, otillräckliga mekaniska barriärer eller en horisontell uterus).

Herbicide resistance is the result of an evolutionary process where a population gradually changes from being susceptible to being resistant to an herbicide. The resistance mechanism is usually a change in the biochemistry of the weed, but in some cases changes in morphology or growth rhythm. The change is caused by random mutations. Two resistance mechanisms are found in black-grass; target site resistance and metabolic resistance.Black-grass is a tufty, winter annual grass weed, which is mainly found in milder climates. Black-grass is a big problem in Southern Europe.