This master's theses was carried out on assignment from Stockholm Vatten AB as a part of a project developing new waste water treatment techniques. The goal of the theisis has been to evaluate an anaerobic Membrane bioreactor for treatment of waste water from Hammarby Sjöstad. The bioreactor has not been heated and the main interest has been to study the gas production, power consumption and the reduction of organic matter and nutrients.The system has been completed with a reverse osmosis unit and a total of four batch runs have been made with good results. The use of reverse osmosis allows nutrient in the waste water to be reintroduced into circulation as the reverse osmosis concentrate can be used as crop nutrient.The Membrane unit is of VSEP ("Vibratory Shear Enhanced Processing") type and an extensive Membrane test has been conducted. This so called L-test helped determine the most suitable type of Membrane for the system to allow a higher ±ux and thus lower power consumption.

Today, equine breeding is based on performance and conformation, resulting in a vast variation in fertility among different horses, with noticeable economic losses as a consequence. The horse breeding industry is therefore in need of methods that diagnose the spermatozoa concerning their quality and provide the best sperm for AI. The commonly used selection methods for sperm today are: centrifugation, where extender is added, the sample is centrifuged and then resuspended; swim-up self-migration; adherence separation; and density gradient centrifugation. However, none of these methods are in used routinely in practice before inseminating mares with fresh or cooled semen. The aim of this study was to evaluate a new selection method, Single Layer Centrifugation (SLC), and its effect on sperm viability. The viability was analyzed with regard to Membrane integrity and Membrane stability of ejaculated spermatozoa.

In metabolism Mg plays an important role because it affects many cellular enzymes in the body. The energy metabolism and protein synthesis are dependent on Mg as an enzyme activator, Mg is also involved in the synthesis of RNA and DNA. Absorption of Mg primarily takes place over the ruminal epithelium in ruminants. The main storage of Mg is in the bones but that can only be reached if the cow is in great need of calcium or phosphorus, it's a hormonal regulated process that induces catabolism of bones. Free and available sources of Mg are found in soft tissues and in the extra cellular fluids that includes cerebrospinal fluids and blood.

This report describes a thesis done at the Jönköping University of Technology. The aim of this thesis was to create conditions to optimize an existing automatic electrostatic coating system by changing the technology for dispensing lacquer. To select the technology, a study of different dispensing techniques carried out which concluded that the piezoelectric Membrane and electrostatic atomization are the most interesting techniques.In order to perform tests with dispensing techniques a demonstrator has been optimized. The controller and PC software of the demonstrator has been programmed to be able to send out PWM signals that control the dispensers. An adapter card was designed to control the piezoelectric Membrane and a steering link to the electrostatic atomization has been developed for PWM signals up to 500V.The work has laid ground for a demonstrator where further testing of the dispensing technologies can be performed..

In the coming years, organic food waste will be collected in the region of Gästrikland, Sweden. The collection is planned to cover the entire region by the end of year 2007. To start with, smaller amounts are being collected and transported to a central composting plant in Sala. However, a central composting plant in the region of Gästrikland is projected.The objective of this thesis is to evaluate a number of possible methods for composting of organic food wastes regarding their environmental impacts and localization.There are four scenarios for future composting of organic waste that have been evaluated. There are two that consist of Membrane composting, either in Sala or in the region of Gästrikland.

Oxytocin is a hormone which plays a crucial role in many reproductive and behavioural functions. It affects many organs and the classical peripheral targets are the mammary glands during lactation and uterus during labour. Oxytocin receptors have recently been described in a variety of normal tissues and primary cell cultures, but also in neoplastic tissues and established neoplastic cell lines, as breast cancer cell lines. The signalling pathways and biological effects of the oxytocin/oxytocin receptor system seem to depend on species, type of tissue, physiological versus neoplastic state and receptor location within the cell Membrane.The aim of this project was to find out if the cell proliferation in the canine mammary carcinoma cell line CMT-U27 was affected upon stimulation of oxytocin, and to investigate the presence of oxytocin receptors in these cells. The cell proliferation was examined by using an ELISA-kit, where the absorbance measured is relative to the amount of living cells.

Hammarby Sjöstad is a new district in Stockholm with high environmental standard. Stockholm Water Company evaluates a local wastewater treatment plant with cutting edge technology. The first step is an experimental treatment plant (Sjöstadsverket) with four parallel lines of treatment, each with a capacity to treat wastewater from 150 persons. Within the Membrane technology subproject the possibility of using reverse osmosis (usually called RO) to regain nutrients is examined.Reverse osmosis separates the incoming water in a clean permeate and a concentrate that, as the name indicates, is a more concentrated version of the incoming water. Using this method in wastewater treatment, a solution high in nutrients can be obtained without the use of chemical precipitation agent and with no production of sludge.

In the pilot plant at Hammarby Sjöstad, Sjöstadsverket, several new methods are tested in order to achieve a good use of resources. When a new technique is considered it is often the performance of the technique itself, under given conditions, that is evaluated. However, in order to evaluate the overall function the whole picture is needed. With a system analysis it becomes possible to make a comparison where all the positive aspects are put up against the negative ones, for the technique itself as well as its requirements. In this way the influence that minor components have on an entire system can be considered.This report presents a system analysis of an anaerobic Membrane reactor (MBR) with a VSEP-Membrane (Vibratory Shear Enhanced Process).

Swedish biogas is currently produced mainly by anaerobic digestion of sewage sludgeat sewage treatment plants, landfills and anaerobic digestion of household or industrialwaste. Also cattle ma-nure from farms can be used to produce biogas. Upgradingbiogas represents an increase in methane concentration from about 65 % to about 97%. In addition, particles and contaminants must be sepa-rated from the upgradedbiogas. There are various methods for upgrading and this report compares sixdifferent methods with respect to environment and economy: water scrubber,Biosling, cryo technology, chemical absorption, Pressure Swing Adsorption (PSA) andMembrane separation.According to the used ORWARE model, chemical adsorption with amine has the leastenvironmen-tal impact (methane emissions) and the lowest costs.

Results have shown that flow batteries may be a solution in the future as an effective and environmental friendly method to an energy storage system (ESS). The technology is reliable and has a high efficiency that comes with low energy losses and a long lifetime. The range of possible fields is suitable for cutting energy peaks in the power grid, by always have a ready and available energy storage that balances the production. By comparing the advantages of flow batteries with conventional batteries it is mainly the fact that they can conserve energy for a long time without being self-discharged thanks to that the storage capacity is in principle endless and limited by the size of the electrolytes tanks that makes them a great energy storage system. The batteries won?t take any damage or decrease in performance when charging or discharging it or if you exhausts it to 100 % and leave it discharged for a long time.

The Annexin-V/propidium iodide (AV/PI) assay detects early Membrane changes in spermatozoa and viability using flow cytometry. It is an objective method that evaluates a much larger number of single spermatozoa compared with the usual estimation of motility, the most commonly used method for routine sperm evaluation. In the present study four ejaculates from four stallions were used in a split-sample design to investigate whether and how the number of intact spermatozoa is affected over time and after incubation at different temperatures, either those recorded during shipment of stallion semen for AI (9 or 5°C) or room temperature (20°C). The Annexin-V results did not reflect the motility results that were monitored in parallel. While motility decreased over time, the number of spermatozoa with intact Membranes (viable) remained unchanged for at least 21 hours following a short period of initial instability.

Membrane bioreactors (MBR) combined with biological phosphorous treatment (Bio-P) has been investigated if that could be an alternative to the future expansion with conventional active sludge treatment at Lundåkra wastewater treatment plant (WWTP) in the proximity of Landskrona, Sweden.The results showed that the surface demand in the biological treatment will be reduced with 67% with the MBR-alternative compared to a conventional activated sludge process. The nutrient removal in the MBR-solution was decreased or unchanged for nitrogen-, phosphorous- and organic parameters. However, the energy demand will increase with an estimated cost of approximately 1 million SEK annually. Moreover, the extra chemical consumption is estimated to cost 370 000 SEK annually.The disturbances caused by filamentous bacteria such as sludge dispersal could be resolved with MBR-technology because the Membranes are not affected by these organisms. The Bio-P process combined with MBR-technology works if the wastewater recirculates from the Membranes to an aerobic zone.In the proposal, there will be a new pre-treatment step, for instance hole screens.

I Sverige fanns det 2001 drygt 850.000 fastigheter, varav 500.000 permanentboenden, som saknade anslutning till kommunala VA-anläggningar. Enligt beräkningar gjorda för år 2000 stod de enskilda avloppen för 20% av utsläppen av fosfor till den svenska naturen och 4% av de totala utsläppen av kväve. Den 1:a augusti 2006 trädde naturvårdsverkets allmänna råd om små avloppsanläggningar i kraft, vilka ska vara rådgivande för hur miljöbalken skall tolkas. Enligt de allmänna råden skall avloppsanläggningar som ligger inom hög skyddsnivå klara en reduktion på 90% för fosfor och syreätande ämnen och 50% för kväve. Hans Huber AG i Tyskland utvecklade under 2000 en membranbioreaktor som kan monteras i en befintlig trekammarbrunn, Membrane Clear Box® (MCB).

Wastewater treatment is increasingly controlled by the Swedish environmental quality objectives, adopted by the Swedish parliament. There is a need for a sewage system that can fulfill stringent requirements concerning the environment, recycling and resource use and hygiene. Additional environmental aspects in recent years are the impact of climate change, new knowledge about the effects of pharmaceuticals in water, the need for improved wastewater treatment and increased recycling of nutrients to agriculture. This report has, from a future scenario of the year 2050, analyzed seven different systems for wastewater treatment and treatment of compostable biowaste from households in Eskilstuna. A future scenario was chosen based on that within a forty year period new challenges may arise which can result in higher demands on sewage treatment than those we see today. Another reason why the future scenario was established is that changes in wastewater treatment systems are slow to establish. The analysis is mainly focused on the economical aspects but environmental aspects such as emissions to receiving waters and potential recycling of plant nutrients has also been evaluated briefly.

Nanofiltration is a proper way of preparation of drinking water. The process has a lot in common with reverse osmosis where a semipermeable Membrane is used to separate a pure water flow from the remaining water. A pilot plant was used to ensure that nanofiltration is a suitable technology for a specific waterworks station in Glemmingebro, Ystad. The purpose was also to find out if a pH-adjustment is needed for feed water in a possible full scale plant.  In this case nanofiltration is used mainly to remove sulphate and calcium since the raw water contains concentrations of these substances which exceed the limits for potable water. It is important to receive a high recovery (RC), otherwise the deliviery of water won?t be high enough.